Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation and application of type 1 bovine viral diarrhea virus virus-like particles (BVDV-VLPs)

A bovine viral diarrhea, virus-like technology, applied in the direction of viruses, applications, viral peptides, etc., can solve the problems of weak cellular immune response, indistinguishability, and difficulty in monitoring BVDV.

Active Publication Date: 2021-06-15
CHINA AGRI UNIV
View PDF11 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although inactivated vaccines are highly safe, compared with attenuated vaccines, their ability to induce cellular immune responses is weaker
In addition, BVDV-vaccinated cattle are serologically indistinguishable from BVDV-infected cattle, which makes BVDV surveillance on farms difficult

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation and application of type 1 bovine viral diarrhea virus virus-like particles (BVDV-VLPs)
  • Preparation and application of type 1 bovine viral diarrhea virus virus-like particles (BVDV-VLPs)
  • Preparation and application of type 1 bovine viral diarrhea virus virus-like particles (BVDV-VLPs)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1, the preparation of bovine viral diarrhea virus type 1 virus-like particles

[0063] 1. Expansion of BVDV NADL strain

[0064] 1. Recovery of MDBK cells

[0065] Take out a frozen MDBK cell from liquid nitrogen and quickly place it in a 37°C water bath. After it melts, transfer the cell culture solution to a sterile 15ml centrifuge tube with a pipette gun, and add 10 times the volume of DMEM complete medium , centrifuge at 1 000×g for 10 min, carefully remove the supernatant, resuspend the pellet with complete DMEM medium, pipette 10 μl of the cell solution into a PCR tube, then add 10 μl of 4% trypan blue staining solution and mix well, then pipette 10 μl of the mixture Add it dropwise to the cell counting plate, count the cells with a cell counter, and adjust the cell concentration to 10 with DMEM complete medium after the counting is completed. 6 cells / ml, then transfer 5ml of the cell suspension to a T25 cell culture flask, place at 37°C, 5% CO 2 The...

Embodiment 2

[0169] Embodiment 2, the application of BVDV-VLPs

[0170] 1. Immunogenicity analysis of BVDV-VLPs

[0171] 1. Immunity

[0172] The animal experiment scheme is shown in Table 1 below.

[0173] Table 1 is the animal experiment scheme

[0174]

[0175]

[0176] 5 μg VLP+ISA201: The purified BVDV-VLPs solution prepared in Example 1 is adjusted to a concentration of 20 μg / ml with TNE buffer, preheated to 32°C together with ISA 201VG adjuvant, take two 5ml sterile syringes, and inhale 2ml of BVDV respectively -VLPs and ISA 201VG adjuvant, connect the two syringes with a sterile hose of about 3cm, first slowly push back and forth 20 times (4s / time), then quickly push back and forth 60 times (<1s / time), emulsify well The vaccine was left standing at 20°C for 1 hour; the antigen content of the BVDV-VLPs vaccine prepared in this way was 10 μg / ml;

[0177] 10 μg VLP+ISA201: It is basically the same as the above-mentioned 5 μg VLP+ISA201 method, the difference is that the puri...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses preparation and application of type 1 bovine viral diarrhea virus virus-like particles (BVDV-VLPs). The invention provides the type 1 BVDV-VLPs whcih are non-infectious virus particles composed of bovine viral diarrhea virus Erns protein and E2 protein. The BVDV-VLPs are prepared by using a baculovirus expression system (BEVS), the BVDV-VLPs are circular particles with the diameter of about 50 nm, and Erns and E2, which participate in assembly, exist in the form of homodimers. After a mouse is immunized by the BVDV-VLPs, the mouse can be induced to generate a humoral immune response level which is the same as that of an inactivated vaccine, and the humoral immune response level is higher than the cellular immune response level of the inactivated vaccine.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the preparation and application of bovine viral diarrhea virus type 1 virus-like particles. Background technique [0002] Bovine viral diarrhea virus (BVDV) is a member of the Flaviviridae family and the genus Pestivirus. Members of the same genus include swine fever virus (CFV) and sheep border virus (SBV) . BVDV has an envelope, the particle size is about 50nm, and the genome is a single-stranded positive-strand RNA with a total length of about 12.3kb. It consists of a 5'untranslated region (UTR), a 3'untranslated region (NCR) and a coding sequence of about 3,988 Comprising an open reading frame (ORF) of amino acids, the 5'UTR contains a highly conserved internal ribosome entry site (IRES). ORFs are processed by cellular and viral proteases to generate 11 functional proteins: NH2-Npro, C, E rns , E1, E2, p7, NS2-3 (NS2 and NS3), NS4A, NS4B, NS5A, NS5B. BVDV has three genotypes: B...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/18C12N15/866C12N15/62A61K39/12A61P31/14A61P1/12
CPCC07K14/005C12N15/86A61K39/12A61P31/14A61P1/12C12N2770/24323C12N2770/24351C12N2770/24334C12N2710/14143C12N2800/105C07K2319/02A61K2039/5258A61K2039/552
Inventor 吴文学王展慧彭辰
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com