Tubeimoside with immunologic adjuvant effect as well as preparation method and application of tubeimoside

Abstract

The invention relates to tubeimoside with an immunologic adjuvant effect, a preparation method of the tubeimoside and application of the tubeimoside as a vaccine adjuvant. The saponin is tubeimoside B, tubeimoside C or tubeimoside total saponin containing the two saponin compounds prepared from traditional Chinese medicine tubeimoside B and tubeimoside C, has the effect of an immunologic adjuvant, and can induce an organism to simultaneously generate Th1 type and Th2 type immune responses to antigens; compared with the known alumina gel adjuvant in the prior art, the alumina gel adjuvant can generate stronger cellular immunity and humoral immunity response, and can be used as a vaccine adjuvant. The method for preparing tubeimoside is simple in process, simple and convenient to operate, high in product yield and purity, free of use of first and second organic solvents, free of harmful solvent residues, good in safety and low in production cost.

Description

technical field [0001] The present invention relates to tubeimu saponin with immune adjuvant function, its preparation method and application. Background technique [0002] Vaccination is the most economical and effective measure to control and prevent infectious diseases [O'Hagan DT, Lodaya RN, Lofano G. The continued advance of vaccine adjuvants–'we can work it out'. Semin. Immunol. ]. Due to the prevalence of complex pathogens such as SARS-CoV-2, vaccine development is facing increasing challenges. Adjuvant is an essential component of traditional vaccines and new vaccines. It not only affects the strength of the body's immune response to vaccines, but also induces the most effective type of immune response against specific pathogens [HarandiAM.Systems analysis of human vaccine adjuvants.Semin.Immunol .2018, 39, 30–34; Rappuoli R, Hanon E. Sustainable vaccine development: a vaccine manufacturer's perspective. Curr. Opin. Immunol. 2018, 53, 111–118]. There are many type...

AI Technical Summary

Problems solved by technology

Aluminum glue is still the most widely used adjuvant in vaccine production, and it has some problems: (1) It mainly produces antibodies by inducing Th2 type immune response to play a protective role, and it cannot induce Th1 type immune response and cell-mediated immune response. For vaccines with antibodies as the main protective immunity; (2) No adjuvant effect on various antigens such as human immunodeficiency virus, hepatitis C virus, herpes simplex virus, influenza virus, schistosomiasis, whooping cough and typhoid fever; (3) Promote the production of IgE antibodies , the body is prone to allergic reactions; ⑷ local reactions, the formation of granulomas, and even local aseptic abscesses; ⑸ aluminum gel degeneration after the vaccine containing aluminum gel is frozen; 6) affect the nervous system of the body

However, QS-21 has serious defects, such as ① strong hemolysis and high toxicity and side effects; ② there are acyl groups in the molecular structure, which are easy to be hydrolyzed, and its deacylated hydrolyzate loses the ability to induce Th1 type immune response and generate specific CTL. It can only trigger Th2-type immune response; ③ South American saponins are scarce in resources, low in content, large in molecular weight, complex in chemical structure, and difficult to separate and prepare [Wang PF.Natural and synthetic saponins as vaccineadjuvants.Vaccines 2021,9,222]

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