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Bifidobacterium lactis preparation for reducing fat and losing weight and preparation method thereof

A lipid-lowering and weight-loss, Bifidobacterium lactis technology, applied in microorganism-based methods, biochemical equipment and methods, medical preparations containing active ingredients, etc. The problems of high stress mortality and high bacterial mortality, to achieve the effects of bacterial species vitality and quantity recovery and growth, low mortality, and high bacterial species vitality

Pending Publication Date: 2021-06-15
盐城维康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This production method removes the metabolites produced by probiotics in the liquid fermentation process that are helpful for lipid-lowering and weight loss, and the effect of metabolites of Bifidobacterium lactis cannot be reflected in the product. Therefore, the probiotics produced by this method Bacteria products only use the effect of probiotics themselves but fail to take advantage of the effects of their metabolites; in addition, the stress mortality rate of the bacteria is high when dry, and due to the addition of an appropriate amount of dry protection agent or carrier in the wet bacteria, the liquid Fermented products also have a reduced number of cells after drying
[0017] Therefore, the existing probiotic production process has defects such as low strain content, poor fermentation effect, high bacterial mortality during drying, and low bacterial strain viability in the finished product.

Method used

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  • Bifidobacterium lactis preparation for reducing fat and losing weight and preparation method thereof
  • Bifidobacterium lactis preparation for reducing fat and losing weight and preparation method thereof
  • Bifidobacterium lactis preparation for reducing fat and losing weight and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Isolation, strain characteristics and identification of embodiment 1 bacterial strain

[0044] 1.1 Sample collection

[0045] Select healthy infants from 3 months to 1 year after birth who have not used antibacterial drugs recently, and collect samples from their feces, that is, the collected strains come from the intestinal tract of healthy infants.

[0046] 1.2 Isolation of strains

[0047] Take 5-10g of infant feces samples, put them in a sterile 50ml centrifuge tube, add 20ml of sterile normal saline, shake and mix well, put them in an anaerobic incubator at 37°C, let them stand for overnight culture, draw 1ml of the sample solution, and Sterile normal saline was sequentially diluted 10 times to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 For each gradient, 100 μl of bacterial suspension was coated on the improved MRS plate, and placed in an anaerobic incubator at 37°C for upside-down culture. After 48 hours, colonies were selected from the culture plates coated...

Embodiment 6

[0079] In summary, Bifidobacterium lactis of the present invention has the following characteristics:

[0080] (1) The culture grows well under the environment of pH6.5-7.5, and the optimum growth pH is pH7.0;

[0081] (2) It grows well in a temperature environment of 34-40°C, and the optimum growth temperature is 37°C;

[0082] (3) It has the effect of lowering fat and losing weight.

Embodiment 2

[0083] Embodiment 2 prepares Bifidobacterium lactis preparation (liquid method control example)

[0084] 2.1 Medium

[0085] (1) MRS solid medium (for slant culture and plate culture):

[0086] Glucose 20g / L, peptone 10g / L, beef extract 10g / L, yeast extract 5g / L, agar 15g / L, K 2 HP0 4 2g / L, diammonium hydrogen citrate 2g / L, sodium acetate 5g / L, Tween-80 1g / L, MgS0 4 .7H 2 0 0.5g / L, MnS0 4 .4H 2 00.05g / L, L-cysteine ​​hydrochloride 0.5g / L, adjust the pH to 7.0 with 1mol / L NaOH, and sterilize at 121°C for 20min.

[0087] (2) MRS liquid medium (for Erlenmeyer flask culture and fermenter culture):

[0088] Glucose powder 20g / L, peptone powder 10g / L, beef extract 10g / L, yeast extract 5g / L, tomato paste 10g / L, K 2 HP0 4 2g / L, diammonium hydrogen citrate 2g / L, sodium acetate 5g / L, Tween-80 1g / L, MgS0 4 .7H 2 0 0.5g / L, MnS0 4 .4H 2 0 0.05g / L, L-cysteine ​​hydrochloride 0.5g / L, adjust the pH to 7.0 with 1mol / L NaOH, and sterilize at 121°C for 20min.

[0089] 2.2 Preparat...

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Abstract

The invention relates to a bifidobacterium lactis preparation for reducing fat and losing weight and a preparation method thereof. The bifidobacterium lactis is a bifidobacterium lactis Miuyo-11 strain, and the preservation number of the bifidobacterium lactis is CGMCC 20867. The bifidobacterium lactis preparation is large in viable count and high in activity, contains metabolites which are generated by bifidobacterium lactis and have the effects of reducing fat and losing weight, can be used as an auxiliary conditioning article for obese patients, and assists other fat-reducing and weight-losing medicines in exerting excellent effects of reducing fat and losing weight.

Description

technical field [0001] The invention belongs to the technical field of probiotic production, and in particular relates to a bifidobacterium lactis preparation for reducing fat and reducing weight and a preparation method thereof. Background technique [0002] Obesity refers to the obvious overweight and thick fat layer of the body, which is the state of excess accumulation of adipose tissue in the body. The main causes of obesity are: excessive intake of high-calorie food, too little exercise and so on. When the energy intake of the body exceeds the energy consumption, the excess energy of the body is caused, which promotes the accumulation of fat, and obesity is formed; in addition, factors such as disease, environment, psychology, and excessive toxins in the body cause nutritional imbalance and energy metabolism disorder in the body, etc. , can also form obesity. [0003] Obesity not only affects the beauty of the body, but also causes a variety of diseases, such as: hyp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/745A61P3/04A61P3/06C12R1/01
CPCC12N1/20A61K35/745A61P3/04A61P3/06
Inventor 陈大伟童颖佳童群义陈金林吉峰
Owner 盐城维康生物科技有限公司
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