Method and device for synchronously separating active protein in egg white

A technology for the simultaneous separation of active proteins, applied in the preparation methods of ovalbumin, peptides, transferrin, etc., can solve the problems of limited yield of active proteins, and achieve the effects of easy industrial scale-up, shortened process flow, and simple operation

Active Publication Date: 2021-06-18
NANKAI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The method adopts the method of multiple salting-out precipitation combined with resin adsorption to separate six kinds of proteins including ovomucin, ovotransferrin, ovalbumin, egg inhibitor, ovo...

Method used

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  • Method and device for synchronously separating active protein in egg white
  • Method and device for synchronously separating active protein in egg white
  • Method and device for synchronously separating active protein in egg white

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Embodiment 1

[0033] In this embodiment, fresh eggs were washed and removed, and egg white and egg yolk were separated using eggpers. The fresh egg white 200ml was added to 1800 ml of a 0.9% NaCl phosphate buffer to dilute the fresh egg white, slow magnetic for 15 min, centrifuged at 6000 r / min for 25 min, remove macromolecular viscous protein and other impurities precipitation, obtaining The liquid is egg white diluent. The pH to 6.8 of the egg dilute is adjusted with 1.0 M HCI and NaOH. The separation and concentration of three active proteins such as egg white dilute in the egg white dilution in the egg white dilute were then entered into the separation device coupled to the egg white dilute.

[0034] Among them, the active protein synchronous separation device in the egg white is a first-stage structure containing two basic working units for separating the ovarboe, ovantin and lysolar in the pre-treated egg white dilution of pre-treated egg dilutes. Enzymes and other active proteins. The ...

Embodiment 2

[0037] In this embodiment, the active protein synchronous separation device in the egg white uses a first-stage structure containing two basic working units for egg whitening, egg white, egg white, egg white, eggs obtained after the same premiere of Example 1. Separation of reactive proteins such as turning ferrometers and lysozymes. The liquid chamber separator, the ovarin recovery chamber separator and the lysozy enzyme recovery chamber separator are the same as in Example 1, and the ion exchange membrane used is a heterogeneous ion exchange membrane, produced by Zhejiang Qianqiu Environmental Protection Water Treatment Co., Ltd.. The porous filter used is provided by Ande Membrane Separation Technology Engineering Co., Ltd., wherein the porous filter film 1 is a polyether sulfone ultrafiltration membrane having a molecular weight of 50000, and the porous filter film 2 is a polyether sulfone ultrafiltration membrane that is intercepting a molecular weight of 20,000. .

[0038] T...

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Abstract

The invention provides a method and device for synchronously separating active protein in egg white, and belongs to an active protein separation and extraction technology. The separation device formed by coupling membrane filtration and electrodialysis is used for synchronously separating and concentrating lysozyme, ovalbumin and ovotransferrin in the egg white diluent. The synchronous separation device for active protein in egg white comprises a plurality of basic working units, wherein each basic working unit has a structure of three separated chambers, including an ovalbumin recovery chamber, a feed liquid chamber and a lysozyme recovery chamber. According to the active protein synchronous separation method and device, the technical flow for separating and extracting three main active proteins, namely lysozyme, ovalbumin and ovotransferrin, in egg white is shortened, and secondary pollution caused by large consumption of chemical reagents in a multi-step salting-out method is avoided, the problem of membrane pollution in the process of pressure-driven membrane is further effectively relieved, industrial amplification is facilitated, and the purity and yield of the active protein are remarkably improved.

Description

Technical field [0001] The present invention belongs to the field of reactive protein separation extraction, and in particular, the synchronous separation method and apparatus of three major active proteins and lysozymes in egg white. Background technique [0002] my country is the largest egg production country in the world. In 2019, China National Economic and Social Development Statistics Publication Data showed that my country's poultry egg production was 330.9 million tons, accounting for 43% of the world's total production. Although my country's poultry egg resources are rich, the egg production is still in its infancy. The additional value of poultry eggs is low, and there is a considerable gap and insufficient gap and shortcomings in the deep processing and production and utilization of eggs. It has a significant gap between many developed countries in foreign countries. The protein content in egg white accounts for about 11-13%, is an ideal and high quality protein resou...

Claims

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Application Information

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IPC IPC(8): C07K1/26C07K1/34C07K14/77C07K14/79C12N9/36
CPCC07K1/34C07K1/26C07K14/77C07K14/79C12N9/2462C12Y302/01017
Inventor 卢会霞孙鲁芹赵津礼王建友卜绍峰马飞张陇
Owner NANKAI UNIV
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