Method and device for synchronously separating active protein in egg white
A technology for the simultaneous separation of active proteins, applied in the preparation methods of ovalbumin, peptides, transferrin, etc., can solve the problems of limited yield of active proteins, and achieve the effects of easy industrial scale-up, shortened process flow, and simple operation
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Embodiment 1
[0033] In this embodiment, fresh eggs were washed and removed, and egg white and egg yolk were separated using eggpers. The fresh egg white 200ml was added to 1800 ml of a 0.9% NaCl phosphate buffer to dilute the fresh egg white, slow magnetic for 15 min, centrifuged at 6000 r / min for 25 min, remove macromolecular viscous protein and other impurities precipitation, obtaining The liquid is egg white diluent. The pH to 6.8 of the egg dilute is adjusted with 1.0 M HCI and NaOH. The separation and concentration of three active proteins such as egg white dilute in the egg white dilution in the egg white dilute were then entered into the separation device coupled to the egg white dilute.
[0034] Among them, the active protein synchronous separation device in the egg white is a first-stage structure containing two basic working units for separating the ovarboe, ovantin and lysolar in the pre-treated egg white dilution of pre-treated egg dilutes. Enzymes and other active proteins. The ...
Embodiment 2
[0037] In this embodiment, the active protein synchronous separation device in the egg white uses a first-stage structure containing two basic working units for egg whitening, egg white, egg white, egg white, eggs obtained after the same premiere of Example 1. Separation of reactive proteins such as turning ferrometers and lysozymes. The liquid chamber separator, the ovarin recovery chamber separator and the lysozy enzyme recovery chamber separator are the same as in Example 1, and the ion exchange membrane used is a heterogeneous ion exchange membrane, produced by Zhejiang Qianqiu Environmental Protection Water Treatment Co., Ltd.. The porous filter used is provided by Ande Membrane Separation Technology Engineering Co., Ltd., wherein the porous filter film 1 is a polyether sulfone ultrafiltration membrane having a molecular weight of 50000, and the porous filter film 2 is a polyether sulfone ultrafiltration membrane that is intercepting a molecular weight of 20,000. .
[0038] T...
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