Laser desorption ionization mass spectrum matrix, detection method, mass spectrum and application of mass spectrum in neurotransmitter detection
A technology of laser desorption ionization and detection method, applied in the field of mass spectrometry detection, can solve the problems of inability to meet the needs of neurotransmitter detection, long detection time, slow derivatization reaction between substrate and neurotransmitter, etc., to improve accuracy and detection speed , the detection speed is fast, the effect of shortening the detection time
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Embodiment 1
[0051] This embodiment is the detection of mouse neurotransmitters, including dopamine (DA), norepinephrine (NAd), 5-hydroxytryptamine (5-HT), γ-aminobutyric acid (GABA), histamine (H) .
[0052] Prepare 1mmol / L [Ph 3 PAu] 3 o + BF 4 - acetonitrile solution;
[0053] Prepare the acetonitrile solution of 3nmol / L dopamine, norepinephrine, serotonin, γ-aminobutyric acid and histamine respectively;
[0054] At 1mmol / L [Ph 3 PAu] 3 o + BF 4 - Add 0.2 μL of each neurotransmitter solution to 1 μL of the acetonitrile solution to form a mixed solution;
[0055] The mixed solution was stirred with a vortex shaker for 20 minutes. After 20 minutes, the mixed solution was transferred out of the vortex shaker and the reaction was continued for 3 hours. The formation of gold nanoparticles was confirmed when a wine-red colloidal suspension was observed;
[0056] The samples obtained were washed by centrifugation at 12000 rpm for 5 minutes. After centrifugation, the supernatant ...
Embodiment 2
[0068] This example is the same as Example 1, except that the neurotransmitters in the mouse brain sample extract were detected by MALDI-TOF-MS in this example.
[0069] One-year-old male Kunming mice (20-22 g) were housed in individually ventilated cages and maintained in an environment with free access to standard food and water at an ambient temperature of 22°C and a humidity of 50%. All experiments were performed in accordance with the guidelines of the Chinese Society for Laboratory Animal Science to ensure animal welfare.
[0070] The mouse brain extract was prepared as follows:
[0071] After the mouse was anesthetized with 100mg / kg chloral hydrate solution, the animal mouse was executed by decapitation, the head was taken out immediately, irradiated with microwaves, and heated to 80°C within 16 seconds;
[0072] The brain was quickly removed from the cranium and three regions (striatum, hippocampus, and prefrontal cortex) were dissected. Tissues from each region were...
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