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Phylum: Firmicits strain with anti-cancer activity and anti-cancer uses thereof

A Firmicutes, bacteria technology, applied in bacteria, medical raw materials derived from bacteria, anti-tumor drugs, etc., can solve the problems of destruction, cancer cell drug resistance, physical and mental, powerlessness, etc., and achieve good compliance and remarkable performance. Anticancer activity, low side effects

Pending Publication Date: 2021-06-22
刘树林
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Traditional cancer treatment methods mainly include radiotherapy and chemotherapy. The obvious disadvantages of such methods are the drug resistance of cancer cells and the double damage to the body and mind.
Even with the rise of immunotherapy in recent years, it is still immature, there are many unsolved problems, and it is too difficult to implement, so it is urgent to find new anti-cancer methods

Method used

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  • Phylum: Firmicits strain with anti-cancer activity and anti-cancer uses thereof
  • Phylum: Firmicits strain with anti-cancer activity and anti-cancer uses thereof
  • Phylum: Firmicits strain with anti-cancer activity and anti-cancer uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1: the screening of CCPM7651 bacterial strain

[0056] 1 Materials and methods

[0057] 1.1 Screening medium and related solutions

[0058] 1.1.1 GRC001 solid medium formula (1L):

[0059] Starch (potato or maize or soybean or millet or other grain or other plant) 20g, KNO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, NaCl 0.5g, FeSO 4 ·7H 2 O 0.01g, agar 20g; dissolve or suspend starch first, then add other ingredients, remember to shake well after high pressure. Preferably, starch can also be replaced with other examples of suitable carbon sources, including, but not limited to: sugars such as glucose, fructose, sucrose, starch hydrolyzate, cellulose hydrolyzate and molasses; organic acids such as acetic acid, propionic acid , formic, malic, citric, and fumaric acids; and alcohols, such as glycerin. KNO 3 Illustrative examples of other suitable nitrogen sources may also be substituted, including, but not limited to: ammonia, including ammonia gas a...

Embodiment 2

[0071] Example 2: Preparation of bacterial preparations of CCPM7651 strain [i.e. bacterial culture supernatant containing metabolites]

[0072]Experimental bacterial preparation: use the liquid culture obtained from the expanded culture obtained in 2.3 of Example 1, spread plate culture on GRC001 solid medium (GRC001 liquid medium+agar), and then take a single colony from the plate to inoculate 5mL GRC001 In liquid medium, 120 rpm, 14-39°C, culture for 2-14 days, after amplification, centrifuge the bacterial solution at 8000rpm for 10min, and take the supernatant to obtain bacterial preparation (starch medium) for subsequent anti-cancer cell experiments.

[0073] Control bacterial preparation: replace the above-mentioned GRC001 solid medium with LB solid medium (add tryptone, 10 g; yeast extract, 5 g; NaCl, 10 g in 900 mL deionized water. Shake the container to dissolve the solute, add 5 mol / Adjust the pH to 7.0 with L NaOH, then add deionized water to 1 L. Add 15 grams of a...

Embodiment 3

[0074] Embodiment 3: Anticancer activity experiment

[0075] Human ovarian cancer cell line ES2: epithelial ovarian cancer cell line ES2 was purchased from Wuhan Procell Life Science&Technology Co Ltd.

[0076] CCK8: Cell Counting Kit-8 cell counting reagent, Beyotime Biotechnology (Beyotime Biotechnology).

[0077] 3.1ES2 cell culture: plate, 100μl per well, cell density 1x10 5 / mL, after the ES2 cells adhered to the wall, 5 μl of the bacterial preparation product obtained in Example 2 was added thereto, and the control group was an equal volume of GRC001 liquid medium, and then cultivated for 6-24 hours to observe. The morphology of ES2 cells was observed under a microscope.

[0078] The result is as image 3 As shown, the left picture is the ES2 ovarian cancer cells treated with CCPM7651; the right picture is the control treatment; ES2 cells treated with CCPM765 bacterial preparations aggregated, rounded in shape, fell off, and died.

[0079] 3.2 CCK8 test: ES2 cells we...

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Abstract

The invention provides a Phylum: Firmicits strain with anti-cancer activity and an anti-cancer application thereof, and is characterized in that the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.17114, and the strain is a newly discovered bacterium related to bacillus; the invention also provides an anti-cancer bacterial preparation taking the bacterial strain as an active anti-cancer bacterium, a metabolite produced by the bacterial strain, a drug containing the metabolite, a method for culturing the bacterial strain and application of the preparation, the metabolite and / or the drug in cancer resistance. Experimental data shows that the strain can generate anti-cancer active metabolites, and the strain is found in human intestinal tracts, so that the strain has low or no side effects on human bodies, the problems of large side effects, poor patient compliance and the like of current anti-cancer drugs are solved, and the aim of preventing / treating cancers is expected to be achieved by adjusting the balance of intestinal flora.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to a Phylum: Firmicutes bacteria strain with anticancer activity and its anticancer application. Background technique [0002] Cancer, as the number one factor that endangers human health, has always been a difficult problem for the world's medical community. Changes in the lifestyle of modern people in an unfavorable direction, and the emergence of new risk factors in an endless stream, all of these promote the occurrence and development of cancer. [0003] Traditional cancer treatment methods mainly include radiotherapy and chemotherapy. The obvious disadvantages of such methods are the drug resistance of cancer cells and the double damage to the body and mind. Even with the rise of immunotherapy in recent years, it is still immature, there are many unsolved problems, and it is too difficult to implement, so it is imminent to find new anti-cancer methods. Contents of...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74A61P35/00A61P35/02C12R1/01
CPCC12N1/20A61K35/74A61P35/00A61P35/02C12N1/205C12R2001/07
Inventor 刘树林
Owner 刘树林