Vitamin D3 detection electrode based on molecularly imprinted polymer and preparation method

A technology of molecular imprinting and detection electrodes, which is applied in the field of vitamin detection, can solve the problems of vitamin D3 detection and other problems, and achieve the effects of simple operation, long time-consuming and strong anti-interference ability

Pending Publication Date: 2021-06-22
重庆东渝中能实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no method for the detection of vitamin D3 on the market

Method used

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  • Vitamin D3 detection electrode based on molecularly imprinted polymer and preparation method
  • Vitamin D3 detection electrode based on molecularly imprinted polymer and preparation method
  • Vitamin D3 detection electrode based on molecularly imprinted polymer and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] This embodiment provides a method for selecting a functional monomer when preparing a vitamin D3 detection electrode, and the specific steps are as follows:

[0030] A1. Alternative 8 kinds of functional monomers: A, o-phenylenediamine, B, o-phenylenediamine + resorcinol, C, p-phenylenediamine, D, p-phenylenediamine + resorcinol, E, o-aminophenol, F, o-aminophenol + resorcinol, G, aniline, H, aniline + resorcinol.

[0031] A2, prepare 8 kinds of mixture solutions containing different functional monomers: mix the above 8 kinds of functional monomers with vitamin D3, HAc-NaAc (mixed solution of sodium acetate and acetic acid, pH=5.2) containing 70% methanol respectively , to obtain 8 kinds of polymer solutions containing different functional monomers;

[0032] A3, put 8 identical electrode materials in the above 8 kinds of polymer solutions respectively, use cyclic voltammetry to polymerize the 8 kinds of polymer solutions in A2, and measure the cyclic voltammetry of eac...

Embodiment 2

[0036] This example provides a method for preparing a vitamin D3 detection electrode based on molecularly imprinted polymers, as shown in the attached figure 1 Shown:

[0037] (1) Weigh the powder of the template molecule vitamin D3 and dissolve it in 70mL of methanol, shake it to dissolve completely, weigh sodium acetate, add 25mL of ultrapure water, adjust the pH to 5.2 with acetic acid, add functional monomers p-phenylenediamine and resorcinol , set the volume to 100mL; make the molar ratio of final template molecule vitamin D3, functional monomer p-phenylenediamine and functional monomer resorcinol be 0.3mM:4mM:4mM, the concentration of sodium acetate buffer is 0.1M, and adopt High-purity N2 was ventilated for 5 minutes to remove oxygen to obtain a polymer solution, which was kept at room temperature and protected from light for later use.

[0038] (2) Absorb the polymer solution and drop it on the three screen-printed electrodes, use cyclic voltammetry to polymerize it, ...

Embodiment 3

[0041] This example provides another method for preparing a vitamin D3 detection electrode based on molecularly imprinted polymers, as shown in the attached figure 1 Shown:

[0042] (1) Weigh the powder of the template molecule vitamin D3 and dissolve it in 70mL of methanol, shake it to dissolve completely, weigh sodium acetate, add 25mL of ultrapure water, adjust the pH to 5.2 with acetic acid, add functional monomers p-phenylenediamine and resorcinol , set the volume to 100mL; make the molar ratio of final template molecule vitamin D3, functional monomer p-phenylenediamine and functional monomer resorcinol be 0.9mM: 20mM: 20mM, the concentration of sodium acetate buffer is 0.1M, and adopt High-purity N2 was ventilated for 5 minutes to remove oxygen to obtain a polymer solution, which was kept at room temperature and protected from light for later use.

[0043] (2) Absorb the polymer solution and add it dropwise on the three screen-printed electrodes, and use cyclic voltamme...

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Abstract

The invention relates to the technical field of vitamin detection, in particular to a vitamin D3 detection electrode based on a molecularly imprinted polymer and a preparation method thereof. The preparation method of the detection electrode comprises: taking template molecules and functional monomers to be dissolved in a methanol solution, mixed, oscillated and dissolved, and obtaining a polymer solution; placing the electrode material in a polymer solution, and polymerizing the electrode material by cyclic voltammetry to form an imprinting template membrane on the surface of the electrode material; and washing the molecularly imprinted membrane on the surface of the electrode material by using an eluent to elute the template molecules, thereby obtaining the molecularly imprinted polymer electrode for detecting the vitamin D3 molecules. The preparation method adopts an in-situ polymerization method for synthesis and preparation, and overcomes the defect of long time consumption in the traditional technology; by directly obtaining the selective layer on the electrode, namely forming a layer of vitamin D3 molecularly imprinted polymer on the electrode, the preparation method has the advantages of simplicity, rapidness, high efficiency and easiness in operation.

Description

technical field [0001] The invention relates to the technical field of vitamin detection, in particular to a vitamin D3 detection electrode based on a molecularly imprinted polymer and a preparation method thereof. Background technique [0002] Vitamins are a type of trace organic substances that humans and animals must obtain from food in order to maintain normal physiological functions. Although they do not participate in the formation of human cells or provide energy for the human body, they play an important role in the growth, metabolism, and development of the human body. important role. Vitamins have extremely important physiological functions. Seriously insufficient food supply, reduced absorption and utilization, relatively high requirements, and irrational use of antibiotics will all lead to increased requirements for vitamins, so it is necessary to detect vitamins. [0003] For example, Chinese patent CN110779973A discloses a vitamin electrochemical detection met...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/48
CPCG01N27/301G01N27/308G01N27/48
Inventor 陈星云王璐邹玲玲谢静赵荣森何鑫廖彦剑
Owner 重庆东渝中能实业有限公司
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