Method for detecting volatile terpene substances in liqueur
A volatile and terpene-based technology, which is applied in the field of analysis and detection, can solve the problems that the extraction method and detection method are not suitable for dew and wine, and achieve the effect of high accuracy and precision
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Embodiment 1
[0051] Use a clean and dry 100mL volumetric flask to accurately measure 100mL of dew wine sample (liquid temperature 20°C) into a 500mL retort bottle, rinse the volumetric flask with 50mL water three times, put the washing liquid into the 500mL retort bottle, add a few zeolites ( or glass beads), connect the serpentine condenser, use the original volumetric flask used for sampling as the receiver (plus an ice bath), turn on the cooling water (the cooling water should be lower than 15°C), slowly heat and distill, and collect the distillate; When the distillation reaches 99mL, remove the volumetric flask, cover the stopper, and keep it warm in a water bath at 20°C for 30min, then add third-grade water to the mark to obtain the distillate; take 5mL of the distillate, and add 50μL 2-octanol internal standard solution (21.30mg / L), shake well, put on the gas chromatography-mass spectrometer and detect in the full scan mode, and combine the spectrum library for qualitative, determine ...
Embodiment 2
[0056] Embodiment 2: Linalool standard curve drawing and content determination
[0057] The linalool standard solution (15.97mg / L) was diluted with an ethanol solution with a volume fraction of 60%vol to a concentration of 0.01597mg / L, 0.03194mg / L, 0.1597mg / L, 0.3194mg / L, 0.6388mg / L, For standard series solutions of 1.597mg / L and 2.5552mg / L, pipette 5mL into 10mL glass colorimetric tubes respectively, and add 50μL 2-octanol internal standard solution (21.30mg / L), shake well, and conduct GC Measured with an instrument.
[0058] Take the linalool peak area and the internal standard 2-octanol peak area ratio as the ordinate, and take the ratio of the linalool content (mg / L) and the internal standard 2-octanol content (mg / L) in the series of standard solutions as the abscissa Coordinates, draw the standard curve, and get the linear regression equation and correlation coefficient R 2 .
[0059] Using the blank wine sample without linalool in the background, add linalool standard...
Embodiment 3
[0069] Embodiment 3: Nerol standard curve drawing and content determination
[0070]The nerol standard solution (20.0mg / L) was diluted with an ethanol solution with a volume fraction of 60%vol to a concentration of 0.2mg / L, 0.4mg / L, 0.6mg / L, 0.8mg / L, 1.0mg / L, 1.4mg / L standard series solutions, respectively pipette 5mL into 10mL glass colorimetric tubes, and add 50μL 2-octanol internal standard solution (21.30mg / L), shake well, and measure on the machine.
[0071] Take nerol peak area and internal standard 2-octanol peak area ratio as ordinate, take nerol content (mg / L) and internal standard 2-octanol content (mg / L) ratio as abscissa in series standard solution Coordinates, draw the standard curve, and get the linear regression equation and correlation coefficient R 2 .
[0072] Utilize the blank wine sample without nerolidol in the background, add nerolidol standard samples at the levels of D (0.4mg / L), Z (0.8mg / L) and G (1.4mg / L) respectively, each The level was tested 6 t...
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