Multifunctional trichoderma asperellum and application thereof

A technology of Trichoderma and Acanthospores, applied in the application, fungicides, fungi and other directions, can solve the problems of poor continuous cropping obstacles and other problems, and achieve the effect of strong antagonism

Active Publication Date: 2021-06-25
SHANDONG AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problem that single fusarium wilt control or degradation of harmful substances of phenolic acids is not effective in alleviating continuous cropping obstacles, the present invention provides a strain of Trichoderma asperellum capable of inhibiting multiple pathogenic bacteria such as fusarium wilt pathogens. ) T0206 strain, after research, it is found that it has a very good effect on inhibiting Fusarium wilt and other pathogenic bacteria, inhibiting cucumber wilt and other diseases, and degrading phenolic substances. Multifunctional biocontrol microorganisms with degradative ability are of great significance to alleviate the occurrence of crop continuous cropping obstacles caused by fusarium wilt and phenolic acid stress and improve crop yield, and are also the needs of sustainable agricultural development

Method used

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  • Multifunctional trichoderma asperellum and application thereof
  • Multifunctional trichoderma asperellum and application thereof
  • Multifunctional trichoderma asperellum and application thereof

Examples

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Effect test

preparation example Construction

[0045] In one or more embodiments of the present invention, the present invention also provides a method for preparing the above-mentioned microbial preparation, comprising the following steps: fermenting the above-mentioned T. asperellum T0206 strain in a bottled manner to obtain a fermentation product.

[0046] In the above preparation method, the raw material composition of the fermentation medium used for fermentation is: 70-80% of wheat grains, 10-20% of wheat bran, and 10-20% of humic acid, all in mass percentage.

[0047] In the above preparation method, the fermentation conditions are: 25-30°C, culture: 8-15 days; preferably 28°C, culture for 10 days.

[0048] Further, the above preparation method also includes: drying the fermentation culture in the fermentation product, collecting spores and other steps.

[0049] In the third aspect of the present invention, a disease inhibitor is provided, and the disease inhibitor contains Trichoderma aculeatus T0206 strain or / and ...

Embodiment 1

[0072] Example 1: Isolation and identification of bacterial strains

[0073] 1. Isolation of strains:

[0074] The bitter gourd fusarium wilt diseased soil was collected from the experimental base of Shandong Agricultural University in Tai'an City, Shandong Province, and treated by the soil dilution coating method. After the soil sample was diluted gradiently, 100 μL of the dilution was taken as 10 4 、10 5 、10 6 Evenly spread the diluted liquid on the plate containing PDA medium, culture at a constant temperature of 25°C, and observe day by day after 3 days. When mycelium grows, pick the mycelium block and transfer it to a new PDA plate to continue culturing. When the spores are to be produced, single spores are isolated and purified, the purified strains are numbered, and the slant is preserved for future use.

[0075] PDA medium formula: potato 200g, glucose 20g, agar 17g, tap water 1000mL.

[0076] 2. Microscopic structure observation results of Trichoderma aculeatus T0...

Embodiment 2

[0087] Embodiment 2: Determination of antibacterial ability of Trichoderma aculeatus T0206 bacterial strain

[0088] Various pathogenic fungi such as the Trichoderma strain that has stronger antagonistic activity to the Fusarium wilt pathogen in the embodiment 1 step 3 and the various pathogenic fungi such as the cucumber Fusarium wilt pathogen are carried out the plate confrontation test respectively, adopt two points confrontation (experimental parameters refer to embodiment 1 step 3) , with the T0206 strain not inoculated with Trichoderma aculeatus as a control, the colony growth inhibition rate was calculated. The test results are shown in Table 2.

[0089] The inhibitory effect of table 2 T0206 bacterial strains on the growth of different pathogenic bacteria colonies

[0090]

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Abstract

The invention belongs to the technical field of microbial control, and particularly relates to trichoderma asperellum and application thereof. Research results show that the trichoderma asperellum T0206 strain in the invention has good effects on inhibition of a variety of pathogenic bacteria such as fusarium wilt pathogenic bacteria, inhibition of diseases such as cucumber fusarium wilt, and degradation of phenolic acid substances; and the multifunctional biocontrol microorganism with disease prevention capability and phenolic acid substance degradation capability and the microbial preparation of the multifunctional biocontrol microorganism have important significance on relieving crop continuous cropping obstacles caused by fusarium wilt and phenolic acid substance stress and improving the crop yield.

Description

technical field [0001] The invention belongs to the technical field of microbial control, and in particular relates to a strain of multifunctional Trichoderma aculeatus and its application. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] The occurrence of soil-borne diseases such as fusarium wilt and the allelopathy of phenolic acids secreted by roots are the main reasons for the formation of continuous cropping obstacles in protected vegetables. When the plants are planted continuously, the accumulation of phenolic acids released from the root system reaches a certain concentration, which will inhibit the growth of the next plant and reduce the resistance of the plant to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/38A01P3/00A01P21/00C09K17/14A01G13/00A01G7/06C12R1/885
CPCC12N1/14A01N63/38C09K17/14A01G13/00A01G7/06C09K2101/00Y02A40/10
Inventor 高克祥田叶韩程丽媛张修国梁元存刘会香
Owner SHANDONG AGRICULTURAL UNIVERSITY
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