Processing method of human peripheral blood T lymphocytes and immunogen preparation and application

A lymphocyte and processing method technology, applied in the field of human peripheral blood T lymphocyte processing, can solve the problems of complex antigen composition, difficulty in obtaining thymic lymphocytes, and complex production and purification process of ALG products, and achieves a simplified production and purification process. Effect
CN113025571BActive Publication Date: 2022-05-17武汉中生毓晋生物医药有限责任公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
武汉中生毓晋生物医药有限责任公司
Publication Date
2022-05-17

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Abstract

The present invention relates to a treatment method for human peripheral blood T lymphocytes, preparation and application of immunogen preparations and antiserum, in which discarded white blood cell filter discs are used to separate and purify T lymphocytes, and the T lymphocytes are proliferated and cultured, and the isolated T lymphocytes Compared with the cultured T lymphocytes for antigen surface marker identification and activity comparison; then the expanded and cultured highly active T lymphocytes were used as immunogens, and healthy pigs were used to prepare antiserum, and E rosette inhibition experiments and cell Toxicity test results to evaluate the potency after immunization, formulate immunization procedures, and then a large amount of antiserum can be prepared; then anti-plasma is collected for plasma separation and used for the preparation of ALG products. The present invention can solve the restriction problem that fresh whole blood needs to be collected each time in the potency test of raw materials for preparation of anti-human T lymphocyte immunoglobulin and the potency test of finished products, and can also solve the problem of the bottleneck of the raw material of anti-human T lymphocyte immunoglobulin .
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Description

technical field

[0001] The invention relates to the technical field of blood products, in particular to a processing method for human peripheral blood T lymphocytes, preparation and application of immunogen preparations and antiserum. Background technique

[0002] Anti-human lymphocyte immunoglobulin (ALG) is mainly used for the prevention and treatment of immune rejection in clinical organ transplantation, the prevention of graft-versus-host reaction in bone marrow transplantation, and the treatment of aplastic anemia and other diseases.

[0003] Anti-human lymphocyte immunoglobulin products routinely use human thymus lymphocytes as immunogens. After immunizing pigs, the raw material serum of anti-human lymphocyte immune protein products is obtained, and then purified and refined to obtain pig anti-human lymphocyte immunoglobulin preparations.

[0004] However, it is difficult to obtain human thymus lymphocytes as raw materials, and the standardization of titer testing is p...

Claims

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