A method for the rapid detection of biogenic amines in food by surface-enhanced Raman scattering

A surface-enhanced Raman and biogenic amine technology, applied in the field of chemical analysis and detection, can solve the problems of weak SERS signal and few enhancers, achieve high sensitivity, strong specificity, and improve the effect of Raman detection signal

Active Publication Date: 2022-02-15
腾冲市通三泰农业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reason may be that the SERS signal of biogenic amine itself is weak, and there are relatively few studies on enhancers

Method used

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  • A method for the rapid detection of biogenic amines in food by surface-enhanced Raman scattering
  • A method for the rapid detection of biogenic amines in food by surface-enhanced Raman scattering
  • A method for the rapid detection of biogenic amines in food by surface-enhanced Raman scattering

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Determination of biogenic amines in cheese samples

[0029] (1) Preparation of gold nanoparticles

[0030] Add 150 mL of 2.2 mmol / L citric acid solution into a three-necked flask with a condenser, stir at 100 ° C for 15 min, add 1 mL of 25 mmol / L chloroauric acid solution, stir under reflux for 10 min, the color of the solution gradually changes from yellow to blue-gray It turned light pink again, the solution was cooled to 90 °C, 1 mL of 60 mmol / L citric acid solution and 1 mL of 25 mmol / L chloroauric acid solution were added, stirred under reflux for 30 min, the solution turned wine red, cooled to 90 ° C, and 60 mmol / L lemon was added. 1 mL of acid solution and 1 mL of 25mmol / L chloroauric acid solution were stirred under reflux for 30min, the color deepened, and the solution was immediately cooled to room temperature in an ice-water bath to obtain a gold nano-solution;

[0031] (2) SERS detection of histamine and putrescine standard solutions

[0032]Put...

Embodiment 2

[0046] Example 2: Determination of histamine in squid samples

[0047] (1) Preparation of gold nanosolution: the same as in Example 1;

[0048] (2) SERS detection of histamine and putrescine standard solutions and SERS analysis of standard products: the same as Example 1;

[0049] (3) Accurately weigh 10g of the homogenized squid sample (accurate to 0.01g), place it in a 100mL conical flask with stopper, add 20mL of 5% trichloroacetic acid solution, shake and extract for 30min, and transfer it to a 50mL stoppered centrifuge tube , centrifuge at 5000 r / min for 10 min, transfer the supernatant to a 50 mL volumetric flask, extract the residue again with 20 mL of 5% trichloroacetic acid solution, combine the supernatants, and dilute to the mark with 5% trichloroacetic acid to obtain an extract; Pipette 10mL of the extract in a 25mL test tube with a stopper, add 0.5g of sodium chloride and vortex to shake until the sodium chloride is completely dissolved, add 10mL of n-hexane, vor...

Embodiment 3

[0051] Example 3: Determination of histamine in hairtail

[0052] (1) Preparation of gold nanoparticles: the same as in Example 1;

[0053] (2) SERS detection of histamine and putrescine standard solutions, determination of wavenumbers of histamine and putrescine SERS spectral detection and SERS analysis of standard products: the same as Example 1;

[0054] (3) Pretreatment of hairtail samples: the same as step (3) in Example 2;

[0055] (5) Determination of histamine and putrescine content in hairtail samples: the same as Example 1, no histamine and putrescine were detected in the sample.

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Abstract

The invention discloses a method for rapidly detecting biogenic amine in food by surface-enhanced Raman scattering; in the invention, biogenic amine is chemically derivatized with o-phthalaldehyde to form a derivative with fluorescence, and after compounding with gold nanometers, the surface Enhanced Raman scattering detection, gold nanometers not only play the role of enhancing Raman, but also play a role of fluorescence quenching, the Raman scattering intensity of biogenic amine derivatives is greatly improved, and new characteristic peaks appear at the same time, which can occur The biogenic amines in the reaction include histamine and putrescine, but because they have their own characteristic peaks, they can be detected separately; this method is applied to the detection and analysis of histamine and putrescine in food, and the results are consistent with GB5009.208‑2016 Food Safety National The determination method of biogenic amine in standard food is consistent; the detection limit of histamine and putrescine is 1mg / L, and the method has the characteristics of strong specificity, simple operation and rapidity.

Description

technical field [0001] The invention relates to the technical field of chemical analysis and detection, in particular to a method for rapidly detecting biogenic amines in food by surface-enhanced Raman scattering. Background technique [0002] Biogenic amines are a class of nitrogen-containing low-molecular-weight organic bases, which can be divided into aliphatic amines, aromatic amines and heterocyclic amines according to their chemical structures, which are mainly derived from the decarboxylation of free amino acids or the amination and Transamination. Trace amounts of biogenic amines play a key role in controlling cell growth, differentiation and gene regulation, but when the content of biogenic amines exceeds the threshold, it will cause adverse effects on human health. For example, when phenethylamine exceeds 3 mg / kg, it will cause migraine, When histamine exceeds 100mg / kg, it will cause severe poisoning, even life-threatening. Therefore, it is of great significance ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 杨德志李克相李秋兰吴伟杨亚玲
Owner 腾冲市通三泰农业发展有限公司
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