Micron-sized magnetic fluorescent encoding microspheres and preparation method thereof
A technology of fluorescent coding and magnetic microspheres, which is applied in the fields of microsphere preparation, microcapsule preparation, chemical instruments and methods, etc., and can solve problems affecting the stability of quantum dots and fluorescence quantum yield, low magnetic content of microspheres, and poor magnetic response etc. to achieve excellent fluorescence performance, high fluorescence yield, and high magnetic responsiveness
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Embodiment 1
[0034] According to the method described in the patent ZL201810169129.3, the parameters were adjusted to prepare highly cross-linked carboxyl magnetic microspheres with an average particle size of 3 μm and a CV of 3.1%.
[0035] Weigh 2 g of the above magnetic microspheres, and wash them once with absolute ethanol after magnetic separation. Add 200g of isopropanol, 10g of styrene, 2g of methacrylic acid, 0.1g of CdSe / ZnS fluorescent quantum dots, 8g of N,N'-methylenebisacrylamide, 0.2g of azobisisobutyronitrile in sequence, and disperse ultrasonically for 10min After that, add it into the reaction bottle, put it in a water bath at 70°C, stir at 200rpm, and react for 10h. The sample was magnetically separated, washed with isopropanol and deionized water in sequence, and magnetic fluorescent microspheres with carboxyl groups on the surface were obtained, with a size of 3.5 μm.
[0036] figure 1 It is the fluorescence spectrogram of the surface carboxyl magnetic fluorescent mic...
Embodiment 2
[0038] According to the method described in the patent ZL201810169129.3, the parameters were adjusted to prepare highly cross-linked carboxyl magnetic microspheres on the surface, with an average particle size of 4.8 μm and a CV of 2.5%.
[0039] Weigh 2 g of the above magnetic microspheres, and wash them once with absolute ethanol after magnetic separation. Add 200g of acetonitrile, 12g of methyl methacrylate, 5g of acrylamide, 0.2g of CdSe / ZnS fluorescent quantum dots, 8g of ethylene glycol dimethacrylate, and 0.25g of azobisisobutyronitrile in sequence. After ultrasonic dispersion for 10 minutes, add In the reaction bottle, the water bath is 80°C, stirred at 200rpm, and reacted for 10h. The sample was magnetically separated, washed with ethanol and deionized water in sequence, and magnetic fluorescent microspheres with amino groups on the surface were obtained, with a size of 5.6 μm.
[0040] figure 2 It is the SEM image of the surface amino magnetic fluorescent microsph...
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