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Bacteroides vulgatus capable of protecting intestinal permeability and application thereof

A common Bacteroides, viable count technology, applied in the field of microbiology

Active Publication Date: 2021-07-16
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no patent report that Bacteroides vulgaris can protect intestinal permeability

Method used

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  • Bacteroides vulgatus capable of protecting intestinal permeability and application thereof
  • Bacteroides vulgatus capable of protecting intestinal permeability and application thereof
  • Bacteroides vulgatus capable of protecting intestinal permeability and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1: Isolation and screening of Bacteroides vulgaris CCFM1152

[0046] 1. Sample collection

[0047] A stool sample was collected from a 50-year-old middle-aged man in Dieqiao Apartment, Hedong District, Tianjin. The sample was placed in a stool tube filled with 30% glycerin and kept in an insulated box with ice packs. After being brought back to the laboratory, it was quickly placed in- 80 ℃ refrigerator to be separated and screened.

[0048] 2. Isolation and purification of lactic acid bacteria

[0049] (1) Dilution coating: take about 0.5 g of the content preserved in 30% glycerol and add it into a 10 mL centrifuge tube containing 4.5 mL of normal saline under a sterile environment to obtain 10 -1 Diluent, repeat the above dilution steps to obtain 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 Diluent;

[0050] (2) Coating culture: pipette 100 μL of the above 10 -4 、10 -5 、10 -6 The three gradient dilutions were isolated and screened in the human intestinal tract...

Embodiment 2

[0065] Example 2: Effect of Bacteroides vulgaris CCFM1152 on mouse intestinal fluorescein-labeled dextran (FITC)

[0066] 1. Preparation of Bacteroides vulgaris CCFM1152 gavage solution

[0067] Streak the Bacteroides vulgaris CCFM1152 obtained in step 2 of Example 1 on the BHI solid medium and culture it under anaerobic conditions at 37°C for 48 hours to obtain a single colony; pick a single colony on the BHI solid medium and inoculate it into the BHI liquid medium In 37 ℃ anaerobic conditions enrichment culture 18 ~ 20h for activation, continuous activation for two generations, to obtain activated culture; inoculate the activated culture in BHI liquid culture according to the inoculation amount of 2% ~ 5% (v / v) In the culture medium, the bacteria liquid was enriched and cultivated under anaerobic conditions at 37°C for 18-20 hours to obtain the bacteria liquid, which was centrifuged at 8000g for 10 minutes to obtain the bacteria sludge, washed with sterile saline for 3 times...

Embodiment 3

[0084]Example 3: Application of Bacteroides vulgaris CCFM1152

[0085] Bacteroides vulgaris CCFM1152 can be used to prepare milk, and the specific preparation process of milk is as follows:

[0086] (1) Inoculate the secondary purified culture solution of Bacteroides vulgaris CCFM1152 obtained in Example 1 into the culture medium with an inoculum size of 3% (v / v), and cultivate it for 18 hours at a temperature of 37° C. to obtain the bacterium liquid; centrifuged to obtain the bacteria sludge; the bacteria sludge was washed 3 times with pH 7.2 phosphate buffer solution and then resuspended with a protective agent to a concentration of 1×10 10 CFU / mL to obtain a suspension; the suspension was pre-incubated at a temperature of 37°C for 60 minutes and then freeze-dried to obtain a bacterial agent;

[0087] Among them, the preparation method of the culture medium is: add cysteine ​​hydrochloride 1g / L, hemin 0.01g / L and vitamin K1 0.00 2g / L to the brain heart infusion, and the pH ...

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Abstract

The invention discloses a strain of bacteroides vulgatus capable of protecting intestinal permeability and application thereof, and belongs to the technical field of microorganisms. The bacteroides vulgatus CCFM1152 provided by the invention has been preserved in the Guangdong Microbial Culture Collection Centre on November 9, 2020, and the preservation number is GDMCC No.61279. The bacteroides vulgatus CCFM1152 provided by the invention can effectively prevent weight loss and shortened colon length induced by DSS, reduce intestinal permeability and repair colonic mucosa injury. Therefore, the bacteroides vulgatus CCFM1152 has a wide application prospect in the aspect of preparing food and microecological preparations for protecting the intestinal permeability.

Description

technical field [0001] The invention relates to a common Bacteroides strain capable of protecting intestinal permeability and application thereof, belonging to the technical field of microbes. Background technique [0002] Ulcerative colitis (UC) is a typical chronic recurrent idiopathic intestinal inflammation, which can cause colonic mucosal inflammation, rectal bleeding, and diarrhea. Ulcerative colitis is often accompanied by intestinal mucosal inflammation and intestinal epithelial barrier dysfunction, and its main pathological manifestation is increased intestinal mucosal permeability. Studies have found that compared with normal tissues, the structure and function of tight junctions in ulcerative colitis intestinal tissues are altered, and intestinal mucosal permeability is increased. Intestinal mucosal permeability will cause macromolecular substances such as bacteria, lipopolysaccharide and endotoxin to penetrate the damaged intestinal mucosa into the tissue and in...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/741A61P1/04A61P1/00A23C9/127A23C9/152A23L11/65A23L2/02A23L2/52A23L33/135C12R1/01
CPCC12N1/20A61K35/741A61P1/04A61P1/00A23C9/127A23C9/152A23C11/106A23L2/02A23L2/52A23L33/135A23V2002/00A23V2400/123A23V2400/249A23V2200/32
Inventor 翟齐啸陈卫王晨于雷雷陆文伟崔树茂王刚田丰伟赵建新张灏
Owner JIANGNAN UNIV
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