Novel kidney-targeted nano drug delivery system with biomimetic modification of erythrocyte membrane as well as preparation method and application of novel kidney-targeted nano drug delivery system

A technology of red blood cell membrane and nano-drug loading, applied in the field of biomedicine, can solve the problems of inability to reach the lesion site as expected, low biocompatibility, and high immunogenicity

Active Publication Date: 2021-07-20
THE AFFILIATED HOSPITAL OF SOUTHWEST MEDICAL UNIV
View PDF12 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, NPs have high immunogenicity and low biocompatibility. After entering the blood, they will be recognized by the body's mononuclear phagocyte system and quickly cleared.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Novel kidney-targeted nano drug delivery system with biomimetic modification of erythrocyte membrane as well as preparation method and application of novel kidney-targeted nano drug delivery system
  • Novel kidney-targeted nano drug delivery system with biomimetic modification of erythrocyte membrane as well as preparation method and application of novel kidney-targeted nano drug delivery system
  • Novel kidney-targeted nano drug delivery system with biomimetic modification of erythrocyte membrane as well as preparation method and application of novel kidney-targeted nano drug delivery system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The optimization of embodiment 1 preparation technology

[0041] In this example of the present application, PA-NPs were prepared by nano-precipitation method. The basic method is as follows: Weigh prednisolone acetate (PA) and carrier material polylactic-co-glycolic acid (PLGA) into a 10mL EP tube, add organic Solvent, sonicated to make it completely dissolved, as the organic phase. Weigh the surfactant into a beaker, add ultrapure water, stir to dissolve it completely, and use it as the water phase. The organic phase was quickly added to the aqueous phase, and ultrasonically processed by an ultrasonic cell disruptor with a power of 60W. Stir at low speed at room temperature, volatilize and remove the organic solvent, and obtain PLGA nanoparticles loaded with PA.

[0042] Organic solvent: On the premise of keeping the preparation process and other components unchanged, the experiment prepared nanoparticles by selecting different organic solvents as the organic phase ...

Embodiment 2

[0054] Preparation and application of embodiment 2 RBC-PA-NPs

[0055] 1. Preparation of PA-NPs

[0056] Weigh 2.00 mg of prednisolone acetate (Prednisolone acetate, PA) and 12.00 mg of carrier material polylactic-co-glycolic acid (PLGA), dissolve them together in 5 mL of acetone, and ultrasonically dissolve them completely. Prepare a 1% sodium lauryl sulfate solution and dissolve it in 10 mL of water. Under the conditions of ultrasonic power of 60W and ultrasonic time of 7min, the organic phase was quickly added to the aqueous phase, and stirred at room temperature until the organic solvent was completely evaporated, then filtered with a 0.8 μm filter head to obtain PA-NPs. The particle size, Zeta potential, and PDI were measured by scanning with a nanometer particle size potentiometer. Each sample was measured 3 times in parallel, and the collection temperature was room temperature.

[0057] Such as figure 1 , where A. particle size change; B. surface Zeta potential chang...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to view more

Abstract

The invention belongs to the technical field of biological medicines, and particularly relates to a novel kidney-targeted nano drug delivery system with biomimetic modification of an erythrocyte membrane as well as a preparation method and application of the novel kidney-targeted nano drug delivery system. According to the application, polylactic acid-glycolic acid copolymer (PLGA) is taken as a carrier material, prednisolone acetate (PA) is taken as a model drug, and a novel bionic nano drug delivery system (RBC-PA-NPs) with kidney targeting is constructed by utilizing a strategy of coating an erythrocyte membrane on the surface. The drug delivery system combines the biocompatibility of the erythrocyte membrane and the targeting property of the nanoparticles, so that the kidney targeting property of PA is improved, and the glomerulonephritis is treated more effectively. Therefore, evaluation is carried out from the aspects of a preparation process, physicochemical properties, cytotoxicity and uptake, in-vivo targeting and the like of the drug delivery system.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a novel kidney-targeting nanometer drug-carrying system, preparation method and application of bionic modification of red blood cell membrane. Background technique [0002] Glomerulonephritis (GN) is a common immune-mediated kidney disease at home and abroad, which can manifest as glomerular inflammatory response and cell proliferation, and is the main cause of end-stage renal disease. Generally can be divided into primary renal disease and systemic disease secondary nephritis. Although progress has been made in the pathogenesis, clinical manifestations, diagnosis and treatment of GN, the therapeutic effect on most primary GN is still not obvious. In view of the underlying immunological mechanism of the disease, hormones and immunosuppressants are mainly used for clinical treatment. Due to the lack of specificity in the treatment plan, the use of large doses of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K31/573A61K45/06A61K47/46A61K47/34A61P13/12
CPCA61K9/5176A61K9/5153A61K9/5192A61K45/06A61K31/573A61P13/12Y02A50/30
Inventor 周美玲叶云张荣陶
Owner THE AFFILIATED HOSPITAL OF SOUTHWEST MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap