Novel kidney-targeted nano drug delivery system with biomimetic modification of erythrocyte membrane as well as preparation method and application of novel kidney-targeted nano drug delivery system
A technology of red blood cell membrane and nano-drug loading, applied in the field of biomedicine, can solve the problems of inability to reach the lesion site as expected, low biocompatibility, and high immunogenicity
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Embodiment 1
[0040] The optimization of embodiment 1 preparation technology
[0041] In this example of the present application, PA-NPs were prepared by nano-precipitation method. The basic method is as follows: Weigh prednisolone acetate (PA) and carrier material polylactic-co-glycolic acid (PLGA) into a 10mL EP tube, add organic Solvent, sonicated to make it completely dissolved, as the organic phase. Weigh the surfactant into a beaker, add ultrapure water, stir to dissolve it completely, and use it as the water phase. The organic phase was quickly added to the aqueous phase, and ultrasonically processed by an ultrasonic cell disruptor with a power of 60W. Stir at low speed at room temperature, volatilize and remove the organic solvent, and obtain PLGA nanoparticles loaded with PA.
[0042] Organic solvent: On the premise of keeping the preparation process and other components unchanged, the experiment prepared nanoparticles by selecting different organic solvents as the organic phase ...
Embodiment 2
[0054] Preparation and application of embodiment 2 RBC-PA-NPs
[0055] 1. Preparation of PA-NPs
[0056] Weigh 2.00 mg of prednisolone acetate (Prednisolone acetate, PA) and 12.00 mg of carrier material polylactic-co-glycolic acid (PLGA), dissolve them together in 5 mL of acetone, and ultrasonically dissolve them completely. Prepare a 1% sodium lauryl sulfate solution and dissolve it in 10 mL of water. Under the conditions of ultrasonic power of 60W and ultrasonic time of 7min, the organic phase was quickly added to the aqueous phase, and stirred at room temperature until the organic solvent was completely evaporated, then filtered with a 0.8 μm filter head to obtain PA-NPs. The particle size, Zeta potential, and PDI were measured by scanning with a nanometer particle size potentiometer. Each sample was measured 3 times in parallel, and the collection temperature was room temperature.
[0057] Such as figure 1 , where A. particle size change; B. surface Zeta potential chang...
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