Bacteria transfer and preservation culture medium used in transfer process and preparation method thereof

Abstract

The invention discloses a bacteria transfer and preservation culture medium used in the transfer process and a preparation method thereof. The culture medium comprises the following components: beef extract, peptone, sodium chloride, agar, glycerol, aluminum potassium sulfate, malt extract powder, yeast extract powder, ferric ammonium sulfate, calcium lactate, D-cycloserine, argutone, vitamins, casein phosphopeptides, glucose, lanthanum nitrate, erythritol, sterile defiberized sheep blood and distilled water. The preparation method comprises the steps: adding corresponding parts of culture medium components into the corresponding distilled water, dissolving, uniformly mixing, sterilizing at a high pressure, sub-packaging, and preserving and transferring bacteria in the transfer process through the culture medium. The physiological characteristics of a to-be-detected bacterial sample can be well maintained to be basically unchanged, the effects of enriching bacteria and preventing infectious microbe pollution can be further achieved, and compared with a conventional culture medium, the culture medium disclosed by the invention is high in detection rate, low in pollution, satisfied in clinical application effect and good in transfer effect; and the detection positive rate of the microbial sample is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of microorganism detection, and in particular relates to a bacterial transport preservation medium used in the transport process and a preparation method thereof. Background technique [0002] The transfer of microbial samples usually means that the collection and testing of microbial-related samples in clinical practice are not at the same place and at the same time, so it is necessary to safely transport microbial-related samples from the sample collection site to the testing site, and it is necessary to ensure that the microbial samples are in this A process in which its physiological properties remain largely unchanged during transport. [0003] In clinical microbiology testing, it takes some time from the collection of samples to the actual start of culture testing, and this period of time is crucial for the subsequent culture and reproduction of microorganisms. In clinical practice, due to improper pre...

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Problems solved by technology

[0003] In clinical microbiology testing, it takes some time from the collection of samples to the actual start of culture testing, and this period of time is crucial for the subsequent culture and reproduction of microorganisms. In clinical practice, due to improper preservation of samples, even if the best medium is used, it will not It will lead to a great reduction in the positive detection rate. At present, there is still relatively little research on the transportation and storage conditions of microbial specimens in China, and the attention is not high. At present, the relevant detection indicators of microbial samples are used in clinics to assist in the diagnosis of related diseases. Many, it is also very important for the transfer and preservation method of microbial samples during the transfer process

[0004] At present, there are freeze-drying method, semi-solid medium method and simple glycerin preservation method etc. for the strain preservation method commonly used at present, freeze-drying method, preparation process is complicated, needs better equipment condition again; Semi-solid medium preservation method Bacteria cannot be stored for a long time; the simple glycerin preservation method uses glycerin only for antifreeze and moisturizing, and some special strains have a low survival rate in the glycerin stock solution, and the storage time is short and the toxicity is reduced

[0005] Obviously, it is very important to ensure that the physiological characteristics of microbial samples are basically unchanged during the transfer process, and that they can be fully enriched and prevent bacterial contamination. Currently, the transfer of collected microbial samples from the collection site to the testing site There is no better method for the preservation of strains