Formula for tissue culture of ulmus parvifolia

A technology of tissue culture and Ulmus elm, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of low survival rate of transplanting, high production cost, long seedling cultivation period, etc., and achieve high reproduction coefficient, subculture Short period and good seedling quality

Inactive Publication Date: 2021-08-06
JIANGSU ACAD OF FORESTRY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, rooting in the test tube seedling bottle has high production cost, long seedling cycle (taking root and transplanting in the bottle takes 60 days), low transplanting survival rate (the transplanting survival rate of rooted seedling in the tissue culture bottle is lower than 70%), etc. question
At present, the tissue culture technology of Ulmus ultifolia h

Method used

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  • Formula for tissue culture of ulmus parvifolia
  • Formula for tissue culture of ulmus parvifolia
  • Formula for tissue culture of ulmus parvifolia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. Experimental materials and methods

[0037] 1.1 Experimental materials

[0038] The robust seedlings of the new species of Ulmus ultifolia were selected, and unlignified or semi-lignified annual stems with buds were used as explants to detoxify, induce clustered seedlings, and then proliferate and cultivate, and finally carried out rooting culture for strong seedlings. The experiment was carried out in the plant tissue culture laboratory and greenhouse of Jiangsu Academy of Forestry Sciences. This test includes: shoot induction medium, proliferation medium and rooting medium. The temperature of the culture room is 25-2°C, the light intensity is 2000-3000 Lux, and the light is cultivated for 24 hours.

[0039] 1.2 Primary medium

[0040] The starting medium used was MS medium, and improved WPM, specifically modified as follows: Ca(NO 3 )2·4H 2 O684mg·L-1, KNO3 190mg·L-1, C 10 h 3 FeN 2 NaO 8 73.4mg·L-1 and thiamine hydrochloride 0.1mg·L-1 instead of K in the...

Embodiment 2

[0075] Basic medium configuration:

[0076] Ammonium nitrate (NH 4 NO 3 ) 1650mg / L, potassium nitrate (KNO 3 ) 1900mg / L, potassium dihydrogen phosphate (KH 2 PO 4 ) 170mg / L, magnesium sulfate (MgSO 4 ·7H 2 O) 370mg / L, calcium chloride (CaCl 2 2H 2 O) 440mg / L;

[0077] The components of trace elements and their corresponding concentrations are as follows: Potassium iodide (KI) 0.83mg / L, manganese sulfate (MnSO 4 ·H 2 O) 22.3mg / L, zinc sulfate (ZnSO 4 ·7H 2 O) 8.6mg / L, boric acid (H 3 BO 3 )6.2mg / L, sodium molybdate (Na 2 MoO 4 2H 2 O) 0.25mg / L, copper sulfate (CuSO 4· 5H 2 O) 0.025mg / L, cobalt chloride (CoCl 2 .6H 2 O) 0.025mg / L;

[0078] The components of iron salts and their corresponding use concentrations are as follows: disodium edetate (Na 2 EDTA) 37.3mg / L, ferrous sulfate (FeSO 4 ·7H 2 O) 27.8mg / L;

[0079] The components of organic components and their corresponding concentrations are as follows: inositol 100mg / L, glycine (Gly) 2mg / L, thiamine h...

Embodiment 3

[0089] Basic medium configuration:

[0090] The basic medium is MS medium, in which the components of major elements and their corresponding concentrations are as follows: ammonium nitrate (NH 4 NO 3 ) 1650mg / L, potassium nitrate (KNO 3 ) 1900mg / L, potassium dihydrogen phosphate (KH 2 PO 4 ) 170mg / L, magnesium sulfate (MgSO 4 ·7H 2 O) 370mg / L, calcium chloride (CaCl 2 2H 2 O) 440mg / L;

[0091] The components of trace elements and their corresponding concentrations are as follows: Potassium iodide (KI) 0.83mg / L, manganese sulfate (MnSO 4 ·H 2 O) 22.3mg / L, zinc sulfate (ZnSO 4 ·7H 2 O) 8.6mg / L, boric acid (H 3 BO 3 )6.2mg / L, sodium molybdate (Na 2 MoO 4 2H 2 O) 0.25mg / L, copper sulfate (CuSO 4· 5H 2 O) 0.025mg / L, cobalt chloride (CoCl 2 .6H 2 O) 0.025mg / L;

[0092] The components of iron salts and their corresponding use concentrations are as follows: disodium edetate (Na 2 EDTA) 37.3mg / L, ferrous sulfate (FeSO 4 ·7H 2 O) 27.8mg / L;

[0093] The component...

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Abstract

The invention relates to a formula for tissue culture of ulmus parvifolia. An efficient regeneration system for tissue culture of ulmus parvifolia is successfully established by taking ulmus parvifolia stem segments as explants and utilizing special induction culture, multiplication culture and rooting culture medium formulas of the ulmus parvifolia stem segments. According to the method, the occupied space is saved, the breeding time is not limited by seasons, the subculture period is short, the trouble of plant diseases and insect pests is avoided, the method is more suitable for preservation and rapid and efficient breeding of wild ulmus parvifolia varieties, the breeding efficiency is high, the seedling quality is good, and the reference technology and method are provided for industrialized seedling production of the ulmus parvifolia. The method has important application value in production and scientific research of ulmus parvifolia seedlings.

Description

technical field [0001] The invention relates to the field of breeding methods of Ulmus ultifolia, in particular to a formula for tissue culture of Ulmus ultifolia. Background technique [0002] Ulmus parvifolia Jacq. is a deciduous tree of the genus Ulmaceae (Ulmaceae). It is also known as mosquito palm tree, peeling elm, and small-leaf elm. Its tree height is 25 meters, and its diameter at breast height can reach 1.2 meters. Samara matures in October. It has strong adaptability and is mainly distributed in the south-central part of North China to East China, South-Central and Southwest China, suitable for South China and Central China, and also distributed in Japan and North Korea. The most suitable habitat is warm climate, fertile soil and well-drained neutral soil. The elm likes light, likes a warm and humid climate, is resistant to drought and barrenness, has low requirements on the pH of the soil, and can grow normally under different soil qualities. Elm elm is a pre...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 周洁王保松张敏黄婧蒋泽平邵安领
Owner JIANGSU ACAD OF FORESTRY
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