Aptamer inverse opal photonic crystal sensing material and method for rapid and ultra-sensitive label-free detection of staphylococcus aureus enterotoxin B
A photonic crystal and inverse opal technology, applied in the measurement of scattering properties, silicon dioxide, silicon oxide, etc., can solve the problems of high instrument requirements, high cost, poor specificity, etc., and achieve high sensitivity, easy operation, and good specificity. Effect
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Embodiment 1
[0045] This example is intended to illustrate the preparation method of the adapter steroid photonic crystal sensing material of the present invention, including the following steps:
[0046] 1) Preparation of polymethyl methacrylate (PMMA) microspheres, 125 ml of dual vapor, 25 ml of methacrylate (MMA) and 0.3 g of potassium sulfate (KPS), 3min in a nitrogen atmosphere After the three flasks were added, stirred at a rate of 300 r / min, and the water bath was taken at 80 ° C for 45 min. After the reaction, centrifuge at different speeds was prepared, and the PMMA microsphere was prepared, and the refrigerator was stored at 4 ° C;
[0047] 2) Preparation SIO 2 Antiobulin photonic crystals were prepared by 10 mL of hydrochloric acid (HCl), and 1 g of hydrochloric acid was placed in a test tube, and 1 g of orthosilicate (TEOS) and 1.5 g of anhydrous ethanol were added to the test tube, and the test tube was placed on the shaker The TEOS solution (TEOS: HCl: C) was obtained at room t...
Embodiment 2
[0057] This example is used to illustrate a method of detecting a glutinous glutinotoxin in milk in milk.
[0058] Specifically, the following steps:
[0059] 1) For the actual sample preoperative, the supermarket purchased by the supermarket is diluted and then centrifuged, and the different concentrations of golden glucococcus bow is added to milk;
[0060] 2) The antibiotic stone photon crystals that modified the gold nanoparticles are prepared according to the first embodiment, and stand at room temperature for 6 h;
[0061] 3) Connect the goldenogacterial enterotoxin B adaptation to the surface of the antibocyine photonic crystal, incubated at 37 ° C for 24 h;
[0062] 4) Then detect the diffraction peak response signal at room temperature using the Maya 2000PRO fiber spectrometer.
[0063] 5) The recovery rate of 92.90% -115.35% by standard curve calculation was compared with the actual addition concentration, and the RSD range was from 2.36% to 4.30%. The results show that ...
Embodiment 3
[0065] This example is intended to illustrate the sensing technique of anti-protein photonic crystals to detect the specificity of the cepletexins of golden plasticin.
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