Red light carbonized polymer dot based on citric acid and benzoylurea and preparation method and application thereof

A technology of benzoyl urea and polymer dots, which is applied in the application field of red light carbonized polymer dots, can solve the problems of less long-wavelength emission carbon dots and imaging interference, and achieves excellent fluorescence properties, high sensitivity and simple preparation method. Effect

Active Publication Date: 2021-08-10
XIHUA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problems in the prior art that there are few long-wavelength emission carbon dots, and the interference of cells and in vivo imaging is large, and propose a red-light carbonized polymer dot based on citric acid and benzoylurea, Its preparation method and application, the purpose of the present invention is to provide a kind of carbonized polymer dots (RCBCPDs) with red fluorescence, its preparation and post-processing method are simple, and can realize efficient and selective cell and in vivo imaging

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  • Red light carbonized polymer dot based on citric acid and benzoylurea and preparation method and application thereof
  • Red light carbonized polymer dot based on citric acid and benzoylurea and preparation method and application thereof
  • Red light carbonized polymer dot based on citric acid and benzoylurea and preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0032] A kind of red light carbonized polymer point RCBCPDs, its preparation method comprises the following steps:

[0033] 1) Mix 0.5g of citric acid and 4g of benzoyl urea into a polyphenol tank, then add 5mL of N,N-dimethylformamide and 0.05g of ammonium fluoride, then transfer the polyphenol tube into a stainless steel jacket , react at 160°C for 24h;

[0034] 2) After cooling the reactant obtained in step 1) to room temperature, it is filtered, centrifuged and washed, and the precipitate is collected for subsequent use;

[0035] 3) Dissolve the precipitate obtained in step 2) in an appropriate amount of high-purity water, and transfer it to an activated dialysis bag with a molecular weight cut-off of 8000, and dialyze at room temperature for 96 hours; after that, transfer the aqueous solution of RCBCPDs to a small beaker and freeze After drying for 48 hours, the RCBCPDs (dark blue powder) were obtained.

[0036] figure 1 This is the transmission electron microscope / hig...

Embodiment 2

[0040] A kind of red light carbonized polymer point RCBCPDs, its preparation method comprises the following steps:

[0041] 1) Mix 2g of citric acid and 1g of benzoyl urea into a polyphenol tank, then add 8mL of N,N-dimethylformamide and 1g of ammonium fluoride, then transfer the polyphenol tube into a stainless steel jacket, React at 220°C for 10h;

[0042]2) After cooling the reactant obtained in step 1) to room temperature, it is filtered, centrifuged and washed, and the precipitate is collected for subsequent use;

[0043] 3) Dissolve the precipitate obtained in step 2) in an appropriate amount of high-purity water, and transfer it to an activated dialysis bag with a molecular weight cut-off of 3500, and dialyze at room temperature for 24 hours; after that, transfer the aqueous solution of RCBCPDs to a small beaker and freeze After drying for 48 hours, the RCBCPDs (brown powder) were obtained.

[0044] Figure 4 The steady-state absorption and fluorescence diagrams of R...

Embodiment 3

[0052] A kind of red light carbonized polymer point RCBCPDs, its preparation method comprises the following steps:

[0053] 1) Mix 1g of citric acid and 6g of benzoylurea into a polyphenol tank, then add 10mL of N,N-dimethylformamide and 0.8g of ammonium fluoride, then transfer the polyphenol tube into a stainless steel jacket, React at 240°C for 24h;

[0054] 2) After cooling the reactant obtained in step 1) to room temperature, it is filtered, centrifuged and washed, and the precipitate is collected for subsequent use;

[0055] 3) Dissolve the precipitate obtained in step 2) in an appropriate amount of high-purity water, and transfer it to an activated dialysis bag with a molecular weight cut-off of 500, and dialyze at room temperature for 120 hours; after that, transfer the aqueous solution of RCBCPDs to a small beaker, freeze After drying for 48 hours, the RCBCPDs (black powder) were obtained.

[0056] The present invention also discloses that it can be used for human lu...

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Abstract

The invention discloses a red light carbonized polymer dot based on citric acid and benzoylurea and a preparation method and application thereof, the raw materials of the red light carbonized polymer dot based on citric acid and benzoylurea comprise citric acid and benzoylurea, and the mass ratio of citric acid to benzoylurea is (0.5-3): (1-6). The N, N-dimethylformamide is used as a solvent, ammonium fluoride is used as a fluorine source additive, the surface polymer state is enriched while the carbon nucleus crystalline state of the RCBCPDs is enhanced, and the RCBCPDs are promoted to have a larger multi-photon absorption cross section after fluorine is introduced, so that a stronger fluorescence imaging effect is displayed in vitro and in vivo. On the basis of a citric acid and benzoylurea carbon dot preparation system, the carbonized polymer dots RCBCPDs with red light are developed, and the carbonized polymer dots are simple in preparation method, excellent in fluorescent property and wide in application prospect. When the RCBCPDs obtained by the invention are applied to cell imaging and in-vivo imaging, the RCBCPDs show interference resistance in cells and mice, and have the advantages of high selectivity, high sensitivity and the like.

Description

technical field [0001] The invention relates to the preparation of fluorescent nanomaterials, specifically a red-light carbonized polymer dot based on citric acid and benzoylurea, a preparation method and application thereof, and belongs to the technical field of application of red-light carbonized polymer dots. Background technique [0002] During the development of more than ten years, carbon dots have attracted the attention of many scholars due to their excellent luminescent properties and superior biocompatibility. Carbon dots synthesized by "top-down" or "bottom-up" methods can usually be approximated as a "core-shell" structure, and graphene quantum dots and carbon quantum dots often have clear and clear carbon cores. structure, its "shell" is composed of many surface functional groups (such as -COOH, -SH, O=C-NH and C=O, etc.), while the carbon core of carbonized polymer dots is far less crystalline than the former two, but The structure of the "shell" is highly com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06A61K49/00C08G69/00
CPCC09K11/06A61K49/0021C08G69/00C09K2211/14Y02B20/00
Inventor 王周玉单飞狮陈翔宇张靖朱银霞廖成霜
Owner XIHUA UNIV
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