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Rapid nucleic acid extraction device and method for extracting nucleic acid

A nucleic acid extraction device and extraction device technology, applied in biochemical cleaning devices, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve problems such as leakage, corrosion of thin films, and impact on nucleic acid detection, and achieve cost reduction Effect

Pending Publication Date: 2021-08-13
宁波康程德诺生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The rapid nucleic acid extraction device for trace clinical samples of this method can complete all operations as long as the simple "three-step suction" is used for nucleic acid extraction; Finally, it must be dried, which increases the detection time. If alcohol components enter the nucleic acid of the test, it will affect the nucleic acid test and cause false negatives.
In addition, prepackaged nucleic acid extraction reagents are a trend in nucleic acid detection, such as Chinese invention patent applications (CN111286448A, CN111286449A) and the invention patent applied by the applicant (patent name: a nucleic acid rapid extraction four-tube device, extraction device and extraction method , a kind of integrated nucleic acid rapid extraction and detection test tube and method, etc.), there are flammable and explosive problems in the pre-packaged test tube containing alcohol products; further, because alcohol is a good solvent, it will corrode the pre-packaged Thin film, other substances are brought into the washing liquid, causing leakage in severe cases
[0004] Furthermore, at present, rapid lysis can only be achieved under heating in sample lysis, so the current lysate system cannot achieve the rapid lysis required by this application.
Further, since proteinase K needs to be added to the current lysate system, the price of proteinase K leads to high cost of detection of nucleic acid molecules

Method used

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  • Rapid nucleic acid extraction device and method for extracting nucleic acid

Examples

Experimental program
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Effect test

Embodiment 1

[0048] A fast nucleic acid extraction device, the extraction device includes a fast nucleic acid extractor, a nucleic acid releasing agent, a washing solution and a nucleic acid eluent; as figure 1 As shown, the rapid nucleic acid extractor of the present application includes a syringe 1 and a nucleic acid adsorption head 2, the nucleic acid adsorption head 2 is provided with a nucleic acid adsorption layer 3, and nucleic acid substances in the lysed liquid are adsorbed on the nucleic acid adsorption layer 3, the described The nucleic acid adsorption column 3 is fixedly arranged in the nucleic acid adsorption head 2 and closes the internal through hole of the nucleic acid adsorption head 2 .

[0049] The reagent formula is shown in Table 1

[0050] Table 1

[0051]

[0052] The packaging specifications of the extraction device are shown in Table 2

[0053] Table 2

[0054]

[0055]

[0056] Operation method

[0057] 1. Cracking:

[0058] Blood sample: Take 100uL wh...

Embodiment 2

[0067] A rapid nucleic acid extraction device, the extraction device comprises a rapid nucleic acid extractor, magnetic beads, nucleic acid releasing agent, washing liquid and nucleic acid eluent; the nucleic acid releasing agent, washing liquid and nucleic acid eluting liquid are pre-packaged in a test tube; as Figure 18 As shown, the end of the rapid nucleic acid extractor is provided with a magnet 4, magnetic beads are added to the nucleic acid release agent, and the nucleic acid material is adsorbed on the component by the magnetic beads.

[0068] The reagent formula is shown in Table 3

[0069] table 3

[0070]

[0071] The packaging specifications of the extraction device are shown in Table 4

[0072] Table 4

[0073]

[0074] Operation method

[0075] 1. Cracking:

[0076] 1. Blood sample: Take 100uL whole blood and add it to the lysis tube 5, mix well and let it stand for 3 minutes. Slowly put the magnetic bar into the lysis tube 5, and pump it back and for...

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Abstract

The invention relates to the technical field of nucleic acid extraction, in particular to a rapid nucleic acid extraction device and a method for extracting nucleic acid. The rapid nucleic acid extraction device comprises a rapid nucleic acid extractor, a lysis solution, a washing solution and an eluent, the eluent is used for eluting and collecting nucleic acid substances adsorbed on the rapid nucleic acid extractor, and the washing solution is one or a mixture of more of silicone oil, paraffin oil, C5-C15 alkane oil and halogenated C1-C15 alkane oil. The washing solution does not contain alcohol, does not need to be aired, does not influence nucleic acid, and ensures quick and effective detection.

Description

technical field [0001] The present application relates to the technical field of nucleic acid extraction, in particular to a rapid nucleic acid extraction device and a method for extracting nucleic acid. Background technique [0002] Medical testing of in vitro samples has the advantages of simplification, automation, and proximity to patient sites, and plays an important role in clinical medicine and related medical research fields. At present, the automation of sample processing and testing is low, which cannot meet the needs of rapid, timely and high-throughput testing of samples in clinical practice. Nucleic acid molecular detection technology has strong advantages in laboratory medicine and clinical research. The column method and magnetic bead method are commonly used for nucleic acid extraction, but there are common problems such as complicated operation, laborious and high cost. [0003] Chinese invention patent application (publication number: CN101684463, publicat...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12Q1/6806
CPCC12Q1/6806C12Q2523/308C12Q2527/125
Inventor 王德明周杰锋
Owner 宁波康程德诺生物医药有限公司
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