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Gordonia rubripertincta, application and pigment production method

A technology of Gordonia crimson and Gordonia, which is applied in the field of microorganisms and its applications, can solve the problems of limited strains and achieve the effects of good stability and simple production process

Active Publication Date: 2021-08-13
XIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the microorganisms capable of synthesizing carotenoids are mainly fungi, microalgae and bacteria, etc., but the strains for industrial production of carotenoids are very limited. Therefore, it is of high interest to continue to screen high-yielding strains of high-quality carotenoids from nature. research and development value

Method used

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  • Gordonia rubripertincta, application and pigment production method
  • Gordonia rubripertincta, application and pigment production method
  • Gordonia rubripertincta, application and pigment production method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1: Isolation and Identification of Carotenoid-producing Gordonia Rubus (GH-1)

[0046] (1) Isolation and purification of carotenoid-producing strains: Take 2 g of Pixian watercress (collected in Pidu District, Chengdu City) in the traditional brewing process in 100 mL of GD medium (GD medium: sucrose 20 g / L; potassium nitrate 4 g Magnesium sulfate 0.4g / L; Ferrous sulfate 0.015g / L; Potassium dihydrogen phosphate 2g / L; Disodium hydrogen phosphate 3g / L; Sodium citrate 2g / L), light, 30 ℃ enrichment culture 5d. Afterwards, 200 μL of bacterial liquid was diluted and spread on GD solid medium, and cultured at 30°C for 3 days. The single colonies that produced pigment were picked and streaked and purified in GD solid medium ( figure 1 Middle panel a), after 3 days of culture at 30°C, single colonies were picked for enrichment culture, and stored in glycerol at -50°C.

[0047] (2) Identification of carotenoid-producing strains: 16S rRNA was used to carry out molecular...

Embodiment 2

[0051] Example 2: Colony morphology and physiological and biochemical characteristics of bacterial strain GH-1

[0052] The bacterial strain GH-1 (Gordonia rubripertincta) isolated by the present invention has a yellow colony on the GD agar medium, is round in shape, opaque, and has bulges and wrinkles. Observed under a microscope, the strain is long rod-shaped. After morphological identification and 16S rRNA sequence developmental tree analysis, the strain was named Gordonia rubripertincta (Gordonia rubripertincta) GH-1.

[0053] The physiological and biochemical results of the isolated bacterial strain GH-1 of the present invention are shown in Table 1 below:

[0054] Table 1 Physiological and biochemical results of strain GH-1

[0055]

[0056] Note: Physiological and biochemical test results ("+" means positive, "-" means negative)

Embodiment 3

[0057] Embodiment 3: the identification process of the pigment produced by bacterial strain GH-1

[0058] (1) Strain GH-1 was inserted into GD medium and cultured at 30°C for 120h. Take the bacterial liquid in a refrigerated centrifuge and centrifuge at 8000rpm for 10min. Wash the cells twice with sterile normal saline, then add acetone reagent to sonicate for 20 minutes, centrifuge, extract, and take the supernatant, which is the crude pigment.

[0059] (2) The purification of crude pigment is carried out by silica gel column chromatography, and the column is packed by petroleum ether wet method. Petroleum ether: acetone = 4:1; petroleum ether: acetone = 1:1; petroleum ether: acetone = 1:4 Gradient elution. The separation effect was tested by thin layer chromatography and high performance liquid chromatography. TLC conditions: developing solvent is petroleum ether: acetone = 1:4. HPLC conditions: C18 chromatographic column, mobile phase A is pure methanol, mobile phase B ...

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Abstract

The invention provides gordonia rubripertincta, application and a pigment production method, and relates to the technical field of microorganisms and application thereof. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.21239. The gordonia rugulosa disclosed by the invention is separated and screened from traditionally brewed Pixian bean paste, and has the capability of producing carotenoids through fermentation. The discovery of the strain provides a new microbial resource for replacing animal and plant raw materials to produce pigments.

Description

technical field [0001] The invention relates to the technical field of microbes and their applications, in particular to Gordonia rubripertincta, its use and a method for producing pigments. Background technique [0002] Pigments are widely used in daily life, and can be divided into synthetic pigments and natural pigments according to their sources. However, synthetic pigments have disadvantages such as carcinogenicity and toxicity. Therefore, it has become an inevitable trend to replace synthetic pigments with natural pigments. Natural pigments widely exist in many plants and microorganisms, but obtaining natural pigments from plants is limited by factors such as low yield and high production cost. The production of pigments by microbial fermentation has gradually become a current research hotspot because of its short production cycle, high yield, and not limited by seasonal factors. [0003] Carotenoid is a general term for a class of natural pigments. In the field of na...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P23/00C12R1/01
CPCC12N1/20C12P23/00
Inventor 张庆唐洁向文良汪杰孙向阳吴敏李婵媛郑淼心姚红梅
Owner XIHUA UNIV
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