A Strain of Streptomyces xg40 and Its Application
A technology of streptomyces and inoculants, applied in the field of microorganisms, can solve the problems of polluting the human natural environment, ecological balance damage, fruit pesticide residues, etc. Effect
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Embodiment 1
[0032] Embodiment 1: the acquisition of streptomyces catenulae (Streptomyces catenulae) XG40 bacterial strain
[0033] Select the rhizosphere soil shaken off by the roots of healthy strawberry plants in the strawberry planting base of Yancuo Town, Longwen District, Zhangzhou City, Fujian Province, shovel off the topsoil layer, take a soil sample at 10-15 cm, and dilute the soil sample with sterile water for 10 3 、10 4 、10 5 、10 6 、10 7 times, using Gao's No. 1 medium to culture at 28 ° C, and using the conventional PDA plate dilution culture method to separate and obtain. The isolated and purified Streptomyces catenulae (Streptomyces catenulae) XG40 was transferred to Gao's No. 1 medium, cultured and multiplied at 28°C to obtain the Streptomyces catenulae XG40 strain. The Streptomyces parvum XG40 strain was deposited in the General Microorganism Center (CGMCC) of the China Committee for the Collection of Microorganisms on September 14, 2020. The preservation number is CGMC...
Embodiment 2
[0035] Embodiment 2: the culture characteristic of Streptomyces parvulus XG40 bacterial strain on various culture medium
[0036] Inoculate the XG40 strains of Streptomyces parvulus in 8 different culture mediums, culture at 28°C for 36-48 hours, colonies begin to appear, and there are light yellow respiratory droplets on the colonies, and spores begin to produce after 96-120 hours. The colonies are smooth, with smooth or wrinkled edges, easy to pick up, with a diameter of 1.8-2.1 cm, and the color of the spore pile is violet to dark gray. The shape of the colony, the color of the spores, the color of the aerial hyphae, the color of the hyphae in the substrate, the production of soluble pigments, and the growth conditions are all different on different media. The plates were observed and compared, and the results are shown in Table 1.
[0037] Table 1 Culture characteristics of bacterial strain XG40 on various media
[0038]
[0039] Note: "—": no soluble pigment, "+": th...
Embodiment 3
[0044] Example 3: 16S rDNA sequence analysis
[0045] After the genomic DNA of strain XG40 was extracted using the bacterial genome extraction kit, the 16S three-segment primers were 518R: 5'-ATTACCGCGGCTGCTGG-3'; 1100R: 5'-GGGTTGCGCTCGTTG-3'; 926F: 5'-AAACTYAAAKGAATTGACGG-3', PCR amplification of 16S rDNA. The PCR amplification products were recovered, and after ligation, transformation, and identification, the positive clones were sent to Sangon Bioengineering (Shanghai) Co., Ltd. for sequencing. The 16S rRNA gene sequence is shown in SEQ ID No: 1, and the full length of the sequence is 1454bp. The resulting sequences were submitted to the GenBank database for BLAST analysis and comparison, and it was found that the strains with high homology to XG40 belonged to the genus Streptomyces, and the 16S rDNA sequences of 17 typical strains were selected to construct a phylogenetic tree with MEGA5.0 software ( figure 2 ). The similarity between strain XG40 and Streptomyces caten...
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