Fluorescence-colorimetric dual-signal detection of Fe<3+> and GSH by MoSe2@Fe nanocomposite material

Abstract

The invention discloses a method for detecting Fe<3+> and GSH by a fluorescence-colorimetric dual-signal sensor based on a MoSe2@Fe nano composite material; the fluorescence intensity of MoSe2@Fe is inhibited by Fe<3+> through adding Fe<3+>, the fluorescence intensity of the material is recovered after GSH is added, and a fluorescence sensor of the MoSe2@Fe nano composite material is constructed; a standard curve is established according to the fluorescence intensity measured by a fluorophotometer to detect the concentrations of Fe<3+> and GSH; TMB is used as a redox indicator for colorimetric use, and after Fe<3+> is added, the Fe<3+> enhances the enzymatic activity of the nano composite material, so that the blue color is deepened; and then the GSH is added, the GSH can directly reduce the oxTMB, the blue color becomes light, and thus the concentrations of Fe<3+> and the GSH are visually detected. The method has the advantages of time saving, labor saving, low detection limit, good accuracy and simplicity and rapidness in detection.

Description

technical field [0001] The invention belongs to the technical field of biological analysis and detection, in particular to a MoSe-based 2 Fluorescence-colorimetric dual-signal sensor of @Fe nanocomposite for detection of Fe 3+ and GSH methods. Background technique [0002] Iron ions (Fe 3+ ) is an indispensable trace element for the human body, an important component of heme and iron-sulfur protein, and plays an important role in many physiological activities such as enzyme catalysis, oxygen transport, metabolism, and transcription regulation. Research has shown that a deficiency or excess of iron ions in the body can lead to problems such as anemia, heart failure and tissue damage. Efficient and sensitive detection of Fe 3+ The method can more quickly find the Fe in the organism 3+ It is also very important in environmental and food testing. So far, Fe 3+ The detection methods are relatively mature, mainly including inductively coupled plasma mass spectrometry, atomi...

AI Technical Summary

Problems solved by technology

However, these proposed analytical methods all use complex and expensive instruments, long sample processing and complicated reaction processes, which are not conducive to practical application.

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