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Injectable high-pressure-resistant high-strength anti-freezing genipin cross-linked gelatin hydrogel and preparation method thereof

A technology of cross-linked gelatin and genipin, which is applied in the field of preparation of injectable high-pressure-resistant and high-strength freeze-resistant genipin cross-linked gelatin hydrogel, which can solve the problems of safety and potential risks, cytotoxicity, permanent It can achieve excellent frost resistance, long-term stability, excellent anti-fatigue and pressure resistance, and simple methods

Active Publication Date: 2021-08-24
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the interior of the hydrogel formed by pure gelatin only relies on the three-dimensional network structure based on physical interactions such as hydrogen bond-based triple helix structure, hydrophobic interaction, and microcrystalline domains, resulting in weak mechanical properties of traditional gelatin hydrogels and prone to permanent damage. Sexual damage, and easily affected by the environment (such as pH, temperature, etc.), it is difficult to realize the application of multiple functions
Existing studies have improved the mechanical properties of gelatin-based hydrogels to varying degrees through improved methods, but there are not many studies on natural macromolecular gelatin hydrogels with strengths above MPa, and there is a lot of room for research.
Moreover, most gelatin composite hydrogels are prone to fracture and have poor fatigue resistance under large compressive strains.
Through the introduction of cross-linking agents (common cross-linking agents include aldehydes, such as glutaraldehyde), chemical cross-linking is formed, which is cytotoxic, has safety and potential risks, and is not suitable for biomedical and other fields

Method used

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  • Injectable high-pressure-resistant high-strength anti-freezing genipin cross-linked gelatin hydrogel and preparation method thereof
  • Injectable high-pressure-resistant high-strength anti-freezing genipin cross-linked gelatin hydrogel and preparation method thereof
  • Injectable high-pressure-resistant high-strength anti-freezing genipin cross-linked gelatin hydrogel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A preparation method for injectable genipin cross-linked gelatin hydrogel with high pressure resistance, high strength and fatigue resistance, comprising the steps of:

[0036] (1) Dissolve 0.01 g of genipin in ethanol solution, shake and dissolve with an oscillator, and prepare a 1wt% genipin solution;

[0037] (2) Dissolve 2.5g of gelatin in 9ml of deionized water, heat and stir at 50°C to dissolve, and prepare a gelatin solution;

[0038] (3) Use a 1ml syringe to absorb 1wt% genipin solution, add it drop by drop (1 drop per second) to the gelatin solution, heat and stir the mixed solution at 50°C for 14 minutes, ultrasonically remove air bubbles, and stand at room temperature for 24 hours to obtain 0.1GP25GEL (genipin mass fraction 0.1wt%, gelatin mass fraction 25wt%).

[0039] (4) Prepare a glycerin solution with a mass ratio (water:glycerol) of 1:2. Soak 0.1GP25GEL in different glycerin solutions for 3h.

[0040] (5) Take out the soaked hydrogel and rinse with d...

Embodiment 2

[0046] A preparation method of high pressure resistance and high strength genipin cross-linked gelatin hydrogel, comprising the steps of:

[0047] (1) Dissolve 0.05 g of genipin in ethanol solution, shake and dissolve with an oscillator, and prepare a 5wt% genipin solution;

[0048] (2) Dissolve 2.5g of gelatin in 9ml of deionized water, heat and stir at 50°C to dissolve, and prepare a gelatin solution;

[0049] (3) Use a 1ml syringe to absorb 5wt% genipin solution, add it drop by drop (1 drop per second) to the gelatin solution, heat and stir the mixed solution at 50°C for 11 minutes, ultrasonically remove air bubbles, and stand at room temperature for 24 hours to obtain 0.5GP25GEL (genipin mass fraction 0.5wt%, gelatin mass fraction 25wt%).

[0050] Such as figure 2 and Figure 4 As shown, the water content of 0.5GP25GEL is 73.5%, the degree of crosslinking is 75.97%; under the strain of 97%, the compressive fracture stress is 10.95MPa, which has high fracture strength. ...

Embodiment 3

[0052] A preparation method for low-strength genipin cross-linked gelatin hydrogel, comprising the steps:

[0053] (1) Dissolve 0.005g of genipin in ethanol solution, shake and dissolve with an oscillator, and prepare a 0.5wt% genipin solution;

[0054] (2) Dissolve 0.5g of gelatin in 9ml of deionized water, heat and stir at 50°C to dissolve, and prepare a gelatin solution;

[0055] (3) Draw up 0.5wt% genipin solution with a 1ml syringe, add it drop by drop (1 drop per second) into the gelatin solution, heat and stir the mixed solution at 50°C for 40 min, remove bubbles by ultrasonic waves, and let stand at room temperature for 24 hours to obtain 0.05GP5GEL (genipin mass fraction 0.05wt%, gelatin mass fraction 5wt%).

[0056] Such as Figure 4 As shown, 0.05GP5GEL has a compressive fracture stress of 0.21MPa under a strain of 75%, and its fracture compressive stress and strain are relatively weak, which are lower than the gelatin hydrogel in the most suitable genipin concent...

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Abstract

The invention belongs to the technical field of hydrogel material preparation, and particularly relates to a preparation method of injectable high-pressure-resistant high-strength anti-freezing genipin cross-linked gelatin hydrogel. The method comprises the steps: firstly, dissolving gelatin to obtain a gelatin aqueous solution; dissolving genipin by using ethanol, dropwise adding a gelatin solution while uniformly stirring and mixing, standing to obtain genipin water gel, and soaking in a glycerol solution to obtain glycerol-genipin gelatin hydrogel. The genipin cross-linked gelatin hydrogel prepared by the method has high strength, freezing resistance and long-term stability, and has excellent fatigue resistance and pressure resistance. The hydrogel added with glycerol can maintain the original properties after long-term storage, can maintain high flexibility at low temperature, and has excellent frost resistance and long-term stability. Meanwhile, the mechanical property of the genipin hydrogel can be regulated and controlled according to different crosslinking degrees, and the prepared hydrogel has injectability and is expected to be widely applied to the fields of biomedical materials and the like.

Description

technical field [0001] The invention belongs to the technical field of hydrogel material preparation, and in particular relates to a preparation method of an injectable high-pressure-resistant, high-strength, anti-freeze genipin cross-linked gelatin hydrogel. Background technique [0002] Hydrogel is a kind of three-dimensional polymer network material containing a large amount of water inside. It is mainly used in food, medicine, materials and other fields, and has broad application prospects in drug delivery, tissue engineering scaffolds, artificial articular cartilage, flexible devices, etc. . As a common hydrogel material, gelatin is the product of collagen hydrolysis by acid, alkali or enzyme. The raw material is cheap, and the formed gelatin hydrogel has good biocompatibility, biodegradability and environmental protection. friendliness. [0003] At present, the preparation and performance research of high-strength hydrogel is still a hot spot. However, the interior ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08L89/00C08K5/1545C08K5/053
CPCC08J3/075C08J2389/00C08K5/1545C08K5/053
Inventor 黄彦黄晓杰翟豫洲唐颖达
Owner FUZHOU UNIV
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