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Preparation method of multicolor fluorescent test strip for detecting chlorothalonil

The technology of chlorothalonil and test strips is applied in the application field of food and environmental samples, and achieves the effects of simple and easy preparation method, excellent specificity, and uniform product size distribution.

Pending Publication Date: 2021-09-03
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few reports in the field of pesticide residue detection of fluorescent ICS developed based on the assembly of multicolor fluorescent QDs into a "traffic light"-like fluorescent ICS.

Method used

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  • Preparation method of multicolor fluorescent test strip for detecting chlorothalonil
  • Preparation method of multicolor fluorescent test strip for detecting chlorothalonil
  • Preparation method of multicolor fluorescent test strip for detecting chlorothalonil

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preparation example Construction

[0028] A multi-color fluorescent test strip for detecting chlorothalonil according to the present invention includes a sample pad, a binding pad, a chromatographic membrane and a water-absorbing pad, the right end of the sample pad is set on the left end of the binding pad, and the binding pad The right end of the chromatographic membrane is erected on the left end of the chromatographic membrane, and the detection line and the control line are sequentially arranged on the chromatographic membrane from left to right, and the left end of the absorbent paper is erected on the right end of the chromatographic membrane; the binding pad is coated with A labeled tracer, wherein the tracer includes cadmium telluride quantum dots I and cadmium telluride quantum dots II, and the competitive immunoassay of cadmium telluride quantum dots I and cadmium telluride quantum dots II with the substance to be detected respectively Reagents and non-competitive immunoassay reagents are labeled, the...

Embodiment 1

[0040] Preparation of CdTe quantum dots (cadmium telluride quantum dots I and cadmium telluride quantum dots II):

[0041] The cadmium telluride quantum dots used in the present invention are synthesized by reflux using 3-mercaptopropionic acid as a stabilizer. Tellurium powder and NaBH 4 (Mass ratio: 3:10) was dissolved in 1.5 mL of deionized water and stirred for 6 h to produce a clear sodium telluride hydride solution. Then add 0.23g CdCl to the three-necked flask 2 , 100 mL distilled water and 210 μL MPA. With stirring, use 2mol L - 1 The pH was adjusted to 11.4 with NaOH solution, and then deoxygenated for 30 min. The above-prepared NaOH solution in N 2 Continuously add in the flask under the condition, then heat reflux to prepare the water-soluble cadmium telluride quantum dot of MPA coating; Wherein, figure 2 is the TEM image of the product, indicating that the product is a nanoparticle morphology; image 3 is the UV-Vis figure of the product, illustrating the ...

Embodiment 2

[0043] Construction of chlorothalonil multicolor fluorescent test strips:

[0044] Quantum dot I and quantum dot II are respectively marked on streptavidin by the active ester method, and then the quantum dots are labeled on the polypeptide through the streptavidin-biotin system; the combination of marking is: the emission light is 525nm The quantum dot I of the chlorothalonil mimetic epitope polypeptide is marked; the quantum dot II with emission light of 634nm is marked on the polypeptide of the anti-chlorothalonil-antibody immune complex; the structure of the chlorothalonil multicolor fluorescent test strip is as follows: attached figure 1 shown

[0045] Use PBS chlorothalonil antibody and rabbit anti-streptavidin antibody as T line and control C line respectively, spray on the NC membrane, the distance between the two lines is 5mm, and dry the NC membrane at 37°C for 1 hour; BSA was sprayed on the binding pad and dried, then the labeled quantum dots I and II were sprayed o...

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Abstract

The invention discloses a preparation method of a multicolor fluorescent test strip for detecting chlorothalonil. The invention belongs to the field of food detection. The preparation method comprises the following specific steps: preparing cadmium telluride quantum dots, and preparing cadmium telluride quantum dots I and II through stirring; marking, mixing, and dropwise adding the cadmium telluride quantum dots I and II onto a test strip sample pad for placing; importing a shot image into Image J software, channel separation, and acquiring two kinds of optical densities of deep color light and light color light; marking the optical density ratio of the deep light and the shallow light as a vertical coordinate and marking the concentration logarithm of the chlorothalonil extracting solution as a horizontal coordinate to establish a standard curve so as to obtain a linear equation, and finally substituting a detection result into the linear equation to calculate the content of the chlorothalonil in the sample. Food and environmental samples are used as detection samples, excellent specificity is shown, and under the condition that the T line of the test strip can be subjected to multi-color display, the actual detection result can be displayed more directly and sensitively.

Description

technical field [0001] The invention belongs to the field of food detection, and in particular relates to a multicolor fluorescent test strip for chlorothalonil residue based on quantum dots and an application method thereof in food and environmental samples. Background technique [0002] Chlorothalonil is a broad-spectrum fungicide that replaces benzene, which can effectively prevent and control diseases of fruits and vegetables. With the increase in dosage over the years, chlorothalonil has become the second largest fungicide in the world. Therefore, the residual chlorothalonil content in the environment and agricultural products is increasing year by year. Chlorothalonil and its metabolites are easily soluble in water, not easy to degrade in nature, have obvious cumulative toxicity, and are highly toxic to fish and aquatic invertebrates. In 2017, researchers found that chlorothalonil has significant carcinogenic and teratogenic effects, so in 2017, the World Health Organ...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558G01N21/64G01N33/533
CPCG01N33/56911G01N33/558G01N21/64G01N33/533G01N21/6486
Inventor 戴志晖盛恩泽朱钦舒
Owner NANJING NORMAL UNIVERSITY