Method for comprehensively utilizing and processing spirulina

A processing method and technology of spirulina, applied in the field of food processing, can solve problems such as complicated operation steps, requirements, and increased production costs

Inactive Publication Date: 2021-09-07
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

"A Method for Extracting Various Active Substances from Spirulina and Its Application" provides a method for extracting various active substances from spirulina, and obtains phycocyanin, protein, γ-linolenic acid, and spirulina through fractional precipitation separation. Polysaccharides, selenoproteins, chlorophyll, carotene and other effective substances, but the operation steps are relatively complicated and not suitable for industrial applications; "A Fractional Separation Method for Biologically Active Substances of Spirulina" provides a method for fractional separation of active substances of Spirulina , the method first extracts phycocyanin peptides, and then obtains glycolipids through supercritical carbon dioxide extraction, and obtains spirulina protein and polysaccharide crude products after drying the residual algae residue, so as to realize the graded utilization of spirulina raw materials
The above-mentioned comprehensive methods all involve the use of organic solvents, which not only increase the production cost, but also have high requirements for production conditions, which make them have limitations in the actual production process

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  • Method for comprehensively utilizing and processing spirulina

Examples

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Effect test

Embodiment 1

[0027] Embodiment 1: for step (1), add 8 times of pure water of spirulina raw material weight, soak time 2h, homogeneous pressure 15MPa, material realizes solid-liquid separation by plate and frame filtration; for step (2), add alkaline protease, alkaline The amount of protease added is 1.0% of the raw material quality of spirulina, which is filtered by ceramic membrane filtration equipment, and the ultrafiltration permeate is concentrated to a solid content of 10% through nanofiltration; for step (3), add alkaline protease and papain, and the amount Respectively 0.6% and 0.2% of the weight of the spirulina raw material, the microfiltration adopts ceramic membrane filtration equipment, and the nanofiltration is concentrated to a solid content of 10%; for step (4) the ultrafiltration concentrate is added to adjust the pH to 2 by adding hydrochloric acid, and the filtered material The pH of the solution was adjusted to 6, and the solid content was concentrated to 10% by nanofiltr...

Embodiment 2

[0035] Embodiment 2: add 10 times of pure water of spirulina raw material weight for step (1), soak time 3h, homogeneous pressure 30MPa, material realizes solid-liquid separation by decanter centrifuge; add neutral protease and papaya for step (2) The amount of protease and neutral protease added is 1.0% of the mass of spirulina raw material, and the amount of papain added is 0.4% of the mass of spirulina raw material. It is filtered by ceramic membrane filtration equipment, and the ultrafiltration permeate is concentrated by nanofiltration to a solid content of 10% %; add neutral protease for step (3), the addition is respectively 0.6% of the spirulina raw material weight, microfiltration adopts diatomite filter, and nanofiltration is concentrated to solid content 12%; for step (4) ultrafiltration Hydrochloric acid was added to the concentrated solution to adjust the pH to 3, the filtered feed solution was adjusted to pH 7, and the solid content was concentrated to 12% by nano...

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Abstract

The invention relates to a method for comprehensively utilizing and processing spirulina, and belongs to the technical field of food processing. The method comprises the following steps of: pretreating, extracting and separating the spirulina, preparing phycocyanin peptide from primary extracting solution through compound enzymolysis, preparing spirulina protein peptide from spirulina residues through the compound enzymolysis, and preparing spirulina polysaccharide from an ultrafiltration concentrated solution through refining and impurity removal. According to the method disclosed by the invention, molecular weight distinguishing of protein and polysaccharide is realized in an enzymolysis mode, meanwhile, by means of the distribution difference of the phycocyanin and the spirulina protein in cell tissues, the soluble phycocyanin is firstly dissolved out in a crushing extraction mode so as to realize separation of the phycocyanin in the extracting solution and the protein in cell walls, and then, the extracting solution and the spirulina residues are respectively subjected to enzymolysis to obtain two products, namely the phycocyanin peptide and the spirulina protein peptide. According to the method disclosed by the invention, the use of organic reagents is avoided, the extraction step of comprehensive utilization of the spirulina product is simplified, and the comprehensive utilization value of spirulina resources is improved.

Description

technical field [0001] The invention relates to a step-by-step comprehensive utilization extraction and processing method for polysaccharides, phycocyanin peptides and algae protein peptides in spirulina, which belongs to the technical field of food processing. Background technique [0002] Spirulina is a spiral-shaped prokaryotic algae of Cyanophyceae Oscillatoriaceae, with a protein content of 60-70%, and also contains phycocyanin, polysaccharides, carotene, vitamins and other nutrients. It is recommended by the Food and Agriculture Organization of the United Nations and the World Health Organization The best food for human beings in the 21st century. The output of spirulina in my country ranks first in the world, about 9,000 tons per year, accounting for more than 60% of the global market. However, it is still downstream in the spirulina industry chain, lacking systematic comprehensive utilization and processing technology, and has not formed a product system with high ad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/795A23L33/18C08B37/00
CPCC12P21/06C07K14/795A23L33/18C08B37/0003A23V2002/00A23V2200/30A23V2200/308A23V2250/55
Inventor 冷凯良苗钧魁刘小芳于源张雅婷
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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