On-site ready-to-use Raman enhanced chip kit and preparation method thereof

Abstract

The invention belongs to the technical field of medical detection, and particularly relates to an on-site ready-to-use Raman enhanced chip kit and a preparation method thereof. The kit comprises a silver nitrate solution storage bottle, a hydrogen peroxide solution storage bottle, a chloroauric acid solution storage bottle, a Pt-sprayed silicon wafer, a clean slide, tweezers and a purified water storage bottle. According to the invention, on-site detection and on-demand instant preparation of the Raman enhanced substrate are realized. The method has the basic characteristics of short use time consumption, wide application range and the like, chemical synthesis auxiliary equipment is not needed, the preparation of a single SERS enhanced chip is completed within 2 minutes, the preparation of a specific detection chip is completed within 5 minutes, and a convenient, rapid, efficient and stable detection result is really realized for a user.

Description

technical field [0001] The invention belongs to the technical field of medical detection, and in particular relates to a ready-to-use Raman enhanced chip kit and a preparation method thereof. Background technique [0002] The kit is currently an important means of testing food safety, biological analysis, and environmental pollution at home and abroad. Diagnostic kits are the final products after raw material procurement and production process transformation. Due to its stable reagents, easy storage, and easy operation, the kit has been widely used in detection in various fields. At present, the actual production kits mainly include nucleic acid purification and protein detection, which not only have the characteristics of simple operation, high efficiency and stability. At the same time, combined with in vitro diagnostic technology, more accurate clinical diagnostic information products and services can be obtained by testing human blood, body fluids, tissue and other iso...

AI Technical Summary

Problems solved by technology

[0003] Up to now, most of the kits are in a specific reaction system, so that the target substance in the sample is specifically combined with the functional components of the reagent. The reaction is based on the principle of antigen-antibody reaction, and finally forms (possibly several Different) antigen-antibody immune complexes are detected. This type of kit is often used in a single application, and it is impossible to achieve multi-sample multi-indicator detection through one kit, and lacks the integration of high-throughput detection chip equipment.

At the same time, the stability and reliability of the enhanced substrate, which is the core part of the Raman enhanced chip, still has major problems. For the detection of low-concentration molecules, active agents or impurity molecules adsorbed on the surface of nanoparticles will generate interference signals, but also It may hinder the contact between detection molecules and nano-metals, so the cleanliness of the SERS chip surface directly affects the Raman detection results

In addition, there are major problems in the actual control of the cost of the kit at present, especially the high one-time investment based on micro-nano processing technology and the high production cost. As a result, the price of the Raman-enhanced chip produced by it cannot meet the market. Convenience and low price requirements for rapid detection technology

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