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Fermentation liquor and fermentation polypeptide with whitening and anti-aging effects and preparation method and application thereof

A fermented liquid and efficacy technology, applied in microbial fermentation and biological fields, can solve the problems of difficult absorption and utilization of enzymes, achieve the effect of improving skin texture, reducing cumbersome procedures and the introduction of organic reagents, and promoting blood circulation and metabolism

Pending Publication Date: 2021-09-21
HEFEI KADIER COSMETIC CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, enzymes as macromolecules are difficult to be directly absorbed and utilized by the skin. Therefore, we propose a fermented liquid with whitening and anti-aging effects, a fermented polypeptide and its preparation method and application

Method used

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  • Fermentation liquor and fermentation polypeptide with whitening and anti-aging effects and preparation method and application thereof
  • Fermentation liquor and fermentation polypeptide with whitening and anti-aging effects and preparation method and application thereof
  • Fermentation liquor and fermentation polypeptide with whitening and anti-aging effects and preparation method and application thereof

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preparation example Construction

[0032] A method for preparing a fermented liquid or fermented polypeptide with whitening and anti-aging effects, mainly comprising the following steps:

[0033] (1) Culture medium preparation

[0034] S1: Use a high-speed pulverizer to crush roses and Polygonatum and mix them in a mass ratio of 1-40:1-40 for later use. Add 1-50 g of the mixed raw materials to 100 mL of fermentation medium, especially preferably 5 g / 100 mL, Three concentrations of 10g / 100mL and 15g / 100mL were used to prepare the fermentation medium, which was sterilized at 121°C for 15 minutes before use;

[0035] Among them, the composition of the fermentation medium: 10.0g / L casein peptone, 8.0g / L beef powder, 4.0g / L yeast powder, 20g / L glucose, 0.2g / L magnesium sulfate, 5.0g / L sodium acetate, 2.0g / L L triamine citrate, 2.0g / L dipotassium hydrogen phosphate, 0.05g / L manganese sulfate and 1.0g / L Tween 80, the pH of the culture medium was adjusted to 6.2±0.2;

[0036] S2: Activate the Lactobacillus plantarum ...

Embodiment 1

[0045] This embodiment provides a method for preparing a fermented liquid or fermented polypeptide with whitening and anti-aging effects, which mainly includes the following steps:

[0046] (1) Culture medium preparation

[0047] Add the pulverized rose flower and Polygonatum to the fermentation medium at a mass ratio of 1:1, and sterilize it for later use;

[0048] (2) Inoculation fermentation

[0049] Inoculate the fermentation medium with 1% volume ratio of Lactobacillus plantarum seed solution and place it in a constant temperature oscillator at 20°C to ferment for 24 hours. After obtaining the primary fermentation product, place it in an ultrasonic instrument for ultrasonic treatment, and add it to the primary fermentation product after ultrasonic treatment. Then inoculate 1% Saccharomyces cerevisiae seed solution by volume and ferment for 2 days in a constant temperature shaker at 10°C, place the secondary fermentation product in an ultrasonic instrument for ultrasonic ...

Embodiment 2

[0055] This embodiment provides a method for preparing a fermented liquid or fermented polypeptide with whitening and anti-aging effects, which mainly includes the following steps:

[0056] (1) Culture medium preparation

[0057] Add the pulverized rose flower and sealwort into the fermentation medium at a mass ratio of 20:40, and sterilize it for use;

[0058] (2) Inoculation fermentation

[0059] Inoculate the fermentation medium with 10% volume ratio of Lactobacillus plantarum seed solution and place it in a constant temperature oscillator at 37°C to ferment for 48 hours. After obtaining the primary fermentation product, place it in an ultrasonic instrument for ultrasonic treatment, and add it to the primary fermentation product after ultrasonic treatment. Then inoculate 8% Saccharomyces cerevisiae seed solution and place it in a constant temperature oscillator at 15°C to ferment for 5 days, place the secondary fermentation product in an ultrasonic instrument for ultrasoni...

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Abstract

The invention relates to fermentation liquor and fermentation polypeptide with whitening and anti-aging effects and a preparation method and application thereof. The fermentation liquor is prepared by taking natural plants with the whitening and anti-aging effects as raw materials, conducting primary fermentation on the raw materials through lactobacillus plantarum and then conducting secondary fermentation on a primary fermentation product through saccharomyces cerevisiae. The fermentation polypeptide is prepared by taking protein in fermentation liquor as a raw material through enzymolysis, and the invention further provides the preparation method and application of the fermentation liquor and the fermentation polypeptide. The mixed rose and rhizoma polygonati powder is subjected to lactobacillus plantarum fermentation and ultrasonic treatment and then subjected to saccharomyces cerevisiae low-temperature fermentation and ultrasonic treatment, the obtained fermentation liquor has a high anti-oxidation effect, polypeptide prepared through enzymolysis of fermentation liquor protein has an inhibition effect on tyrosinase, in addition, the fermentation liquor and the fermentation polypeptide are simple and easy to obtain in the preparation process, no additive or organic reagent is added, and the health micro-ecology of the skin can be improved when the fermentation liquor and the fermentation polypeptide are applied to whitening and anti-aging cosmetics.

Description

technical field [0001] The invention belongs to the field of microbial fermentation and biotechnology, and specifically relates to a fermented liquid with whitening and anti-aging effects, a fermented polypeptide and a preparation method and application thereof. Background technique [0002] The health effect of probiotics was first proposed by Russian scientist Mcctchnikoff that probiotics can prolong life and prevent spoilage bacteria, and can treat intestinal disorders and anti-tumor in medicine. Widely used in health care and food. The Ministry of Agriculture of my country announced 6 kinds of probiotics, yeast, lactobacillus, bifidobacterium, streptococcus faecalis, DM423 Bacillus cereus and SA38 Bacillus cereus. Probiotics have various biological effects and produce beneficial metabolites , such as organic acids; and probiotics produce a variety of nutritional active ingredients such as volatile acids, vitamins, folic acid, niacin and pantothenic acid. On the other ha...

Claims

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Application Information

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IPC IPC(8): A61K8/9794A61K8/9789A61K8/64A61Q19/02A61Q19/08C12P21/06C12P21/00C12P39/00C07K1/30C12R1/25C12R1/865
CPCA61K8/9794A61K8/9789A61K8/645A61Q19/02A61Q19/08C12P21/06C12P21/00C12P39/00C07K1/303A61K2800/782A61K2800/85
Inventor 张冬生周安吴成亮高先亭刘玉梅
Owner HEFEI KADIER COSMETIC CO LTD
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