High-flux rapid detection method for benzo [a] pyrene in blood and application

Abstract

The invention discloses a large-flux rapid detection method for benzo [a] pyrene in blood and application of the large-flux rapid detection method. The method comprises the following steps: 1) constructing a benzo [a] pyrene concentration calculation mathematical model in small-volume sample plasma; 2) preparing detection instruments, reagents and materials; 3) setting instrument conditions and a detection mode; 4) preparing a calibration solution and drawing a standard curve; 5) collecting and pretreating a sample to be detected; 6) performing sample detection; 7) calculating the benzo [a] pyrene concentration of the whole blood of the sample. The invention further discloses application of the detection method, and the detection method is applied to a living body of a small-size experimental animal and is used for long-term, repeated and small-volume blood sampling and detection on the premise of normal metabolism. The method mainly aims at the problems that a blood matrix is complex and the benzo [a] pyrene content of a small-volume sample of a small test animal is small, a liquid-liquid extraction method is adopted, pretreatment is improved, a model, a solvent and steps are optimized, the detection accuracy and flux are improved, the detection cost is reduced, and the detection flux is improved.

Description

technical field [0001] The invention relates to the technical field of benzo[a]pyrene detection and analysis, in particular to a large-throughput rapid detection method and application of benzo[a]pyrene in blood of small-capacity samples. Background technique [0002] Benzoapyrene is a five-ring polycyclic aromatic hydrocarbon with the chemical formula C 20 h 12 , the English abbreviation is BaP. Benzo[a]pyrene is the most toxic carcinogen among polycyclic aromatic hydrocarbons, and was listed as a class I carcinogen by the International Cancer Organization (IRIS) as early as 2012. Benzo[a]pyrene in the environment (air, water and soil) and in food mostly enters the human body through inhalation through mouth and nose, dietary intake and skin contact. Because it is a lipophilic compound, it is easier to accumulate in the human lungs, liver, In the digestive system and blood circulation system, it is harmful to human health. [0003] In order to accurately determine the c...

AI Technical Summary

Problems solved by technology

However, this technical solution still has the following deficiencies: one is that the invention cannot be directly applied to detect the content of benzo[a]pyrene in blood samples due to the different content ranges of the sample matrix and benzo[a]pyrene, and the other is that its The detection method used requires a large volume of detection samples, and the adsorbent used will lead to the loss of detection substances, which has a great impact on the detection results of small-capacity samples; third, the detection data analysis method used requires manual judgment and cannot use mathematics. The model and automatic equipment automatically process; the fourth is that the throughput of sample detection is small, and the reagents used are many types and large in volume, which cannot be adapted to large batches of small-capacity detection samples, synchronous and rapid detection, and digital and accurate detection results

Especially for the blood collection of small experimental animals (mice, rats, guinea pigs, rabbits, poultry, birds, fish, etc.) that require continuous testing, the dose usually cannot be greater than 1mL, otherwise it will affect its normal state. Survival, growth and metabolism, affect the effect of animal experiments

However, the existing blood collection detection methods are not suitable for the detection of samples with a volume of less than 1mL.

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