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Compound microbial agent as well as preparation method and application thereof

A compound microbial agent and microbial agent technology, applied in the field of biological control, can solve the problems of chemical pesticide residues, pathogenic bacteria resistance, etc., and achieve the effects of simple preparation, strong adaptability, and prolonging the effective time

Pending Publication Date: 2021-09-28
CHINA TOBACCO CENTRAL SOUTH AGRICULTURAL EXPERIMENTAL STATION +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the technical problems of pathogenic bacteria resistance and chemical pesticide residue risks caused by the use of chemical pesticides to prevent and control diseases in tobacco planting, the present invention discloses a composite microbial agent and its preparation method and application

Method used

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  • Compound microbial agent as well as preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0022] A composite microbial agent, including microbial agent and protective agent; said microbial agent contains the following components: 0.5×10 9 cfu / g Paenibacillus polymyxa, 0.8×10 9 cfu / g Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), 0.6×10 9 cfu / g Bifidobacterium bifidum, 0.7×10 9 cfu / g Paecilomyces fumosa-roseus (Wize) Brown et Smith), 0.8×10 9 cfu / g Beauveria bassiana (Balsamo) Vuillemin and 0.9×10 9 cfu / g Lactobacillus helveticus (Lactobacillus helveticus); the protective agent comprises the following components by weight: 60 parts of corn flour, 5 parts of brown sugar, 0.5 part of ammonium sulfate, 0.4 part of potassium dihydrogen phosphate and 0.5 part of sulfuric acid heptahydrate Magnesium; the particle size of corn flour is 1.5mm; the mass ratio of microbial agent and protective agent in the composite microbial agent is 5:20.

[0023] A method for preparing said composite microbial bacterial agent, comprising the following steps:

[0024] (1) Str...

Embodiment 2

[0029] A composite microbial agent, including microbial agent and protective agent; said microbial agent comprises the following components: 0.9×10 9 cfu / g Paenibacillus polymyxa, 01.2×10 9cfu / g Bacillus amyloliquefaciens, 1.0×10 9 cfu / g Bifidobacterium bifidum, 1.1×10 9 cfu / g Paecilomyces fumosa-roseus (Wize) Brown et Smith), 1.2×10 9 cfu / g Beauveria bassiana (Balsamo) Vuillemin and 1.3×10 9 cfu / g Lactobacillus helveticus (Lactobacillus helveticus); the protective agent comprises the following components in parts by weight: 80 parts of corn flour, 8 parts of brown sugar, 0.8 part of ammonium sulfate, 0.6 part of potassium dihydrogen phosphate and 0.7 part of sulfuric acid heptahydrate Magnesium; the particle size of corn flour is 3mm; the mass ratio of microbial agent and protective agent in the composite microbial agent is 8:30.

[0030] A method for preparing said composite microbial bacterial agent, comprising the following steps:

[0031] (1) Strain activation: Paeni...

Embodiment 3

[0036] A composite microbial agent, including microbial agent and protective agent; said microbial agent contains the following components: 0.6×10 9 cfu / g Paenibacillus polymyxa, 1.0×10 9 cfu / g Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), 0.8×10 9 cfu / g Bifidobacterium bifidum, 0.8×10 9 cfu / g Paecilomyces fumosa-roseus (Wize) Brown et Smith), 1.0×10 9 cfu / g Beauveria bassiana (Balsamo) Vuillemin and 1.1 x 10 9 cfu / g Lactobacillus helveticus (Lactobacillus helveticus); the protective agent comprises the following components in parts by weight: 70 parts of corn flour, 7 parts of brown sugar, 0.7 part of ammonium sulfate, 0.5 part of potassium dihydrogen phosphate and 0.6 part of sulfuric acid heptahydrate Magnesium; the particle size of corn flour is 2mm; the mass ratio of microbial inoculant and protective agent in the composite microbial inoculum is 6:25.

[0037] A method for preparing said composite microbial bacterial agent, comprising the following steps: ...

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Abstract

The invention relates to the technical field of biological control, in particular to a compound microbial agent as well as a preparation method and application thereof. The compound microbial agent comprises a microbial agent and a protective agent, wherein the microbial agent comprises paenibacillus polymyxa, bacillus amyloliquefaciens, bifidobacterium bifidum, paecilomyces fumosoroseus, beauveria bassiana and lactobacillus helveticus; the protective agent comprises corn flour, brown sugar, ammonium sulfate, monopotassium phosphate and magnesium sulfate heptahydrate; the compound microbial agent disclosed by the invention can improve the soil microenvironment, and can effectively prevent and treat wildfire diseases in the tobacco field production process.

Description

technical field [0001] The invention relates to the technical field of biological control, in particular to a composite microbial agent and its preparation method and application. Background technique [0002] China is the largest tobacco producer in the world today, and the total output and sales of tobacco leaves account for about 30% of the world. At present, tobacco tax has accounted for 1 / 10 of my country's fiscal revenue, and tobacco has become an important economic crop in my country. With the change of cultivation conditions, tobacco pests and diseases are increasingly harmful to tobacco production, causing huge economic losses. According to incomplete statistics, the direct economic loss caused by diseases in the process of tobacco planting in my country is more than 700 million yuan every year. Due to the extensive use of chemical pesticides, the residues of chemical pesticides in tobacco leaves have increased, and the resistance of diseases has increased and bec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14A01N63/22A01N63/30A01N63/20A01P1/00C12R1/12C12R1/07C12R1/01C12R1/79C12R1/225C12R1/645
CPCC12N1/20C12N1/14A01N63/22A01N63/30A01N63/20
Inventor 周志成刘勇军尹华群孟德龙刘天波杨佳蒴段淑辉
Owner CHINA TOBACCO CENTRAL SOUTH AGRICULTURAL EXPERIMENTAL STATION
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