Biosurfactant and preparation method thereof

A biosurfactant and active agent technology, which is applied in the direction of chemical instruments and methods, dissolution, etc., can solve problems such as violations of the production of biosurfactants and insufficient performance of surfactants

Active Publication Date: 2021-10-08
百葵锐(深圳)生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the protein modification method has many advantages such as simple operation method and low production cost, the performance of the obtained surfactant is often not good enough, or biotoxic organic chemicals are used in the modification process, which violates the principle of manufacturing biological surfaces. The original intention of the active agent

Method used

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  • Biosurfactant and preparation method thereof
  • Biosurfactant and preparation method thereof
  • Biosurfactant and preparation method thereof

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preparation example Construction

[0034] In the present invention, the degree of hydrolysis of the proteolysis product is 2%-10%. In the present invention, the preparation method of the proteolysis product preferably includes the following steps: mixing the protein to be hydrolyzed with water to obtain the product to be hydrolyzed, adding protease to the product to be hydrolyzed for enzymolysis, and sterilizing Enzymes to obtain proteolysis products.

[0035] In the present invention, before adding protease to the hydrolyzed product, it is preferred to further include preheating the hydrolyzed product to the temperature of enzymatic hydrolysis.

[0036]In the present invention, when the protease is alkaline protease and the protein to be hydrolyzed is soybean protein, the temperature of the enzymolysis is preferably 55-60°C; the time of the enzymolysis is preferably 0.01-1 h; The process of enzymolysis is preferably carried out in a water bath; during the process of enzymolysis, the pH value of the product to...

Embodiment 1

[0047] The preparation of embodiment 1 soybean protein isolate enzymatic hydrolyzate

[0048] Accurately weigh 5g of soybean protein isolate, dilute to 100mL with water, preheat in a 55°C water bath for 10min, adjust the pH to 8.0, and add 0.05g of alkaline protease for enzymatic hydrolysis. During the enzymatic hydrolysis, 1M NaOH aqueous solution was added dropwise to maintain the pH at 8.0, and the amount of lye added was recorded to determine the degree of hydrolysis. According to the predetermined degree of hydrolysis, after dropping the corresponding amount of lye, immediately inactivate the enzyme in a 95°C water bath for 15 minutes to terminate the enzymolysis reaction, and obtain the enzymolysis product after cooling down in the cold water bath. The degree of hydrolysis DH and the amount of lye added dropwise are shown in the formula I relational formula in the above-mentioned scheme.

[0049] In this embodiment, the relationship between the degree of hydrolysis of t...

Embodiment 2

[0052] Example 2 SDS polypropylene gel electrophoresis detection of soybean protein isolate hydrolyzate

[0053] The enzymatic hydrolysis product in embodiment 1 is carried out SDS polypropylene gel electrophoresis, the result is as follows figure 1 , figure 1 The lanes from left to right in the middle are soybean protein isolate solution, marker, blank control, 2%, 4%, 6%, 8% and 10% hydrolyzed enzymatic products. Depend on figure 1 It can be seen that the particle size of soybean protein isolate is significantly smaller after being enzymatically hydrolyzed, and has a faster migration rate in the gel.

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Abstract

The invention provides a biosurfactant and a preparation method thereof, and belongs to the technical field of surfactant preparation. The biosurfactant is obtained by a chelation reaction of the following raw materials: a proteolysis product, a protein aqueous dispersion and a multivalent metal ion aqueous solution. According to the invention, by compounding a polypeptide product (proteolysis product) obtained after protein is hydrolyzed by enzyme with protein and multivalent metal ions, the performance of the surfactant can be obviously improved by utilizing the synergistic effect of two or more components with different molecular sizes at an interface; on the basis of controlling the hydrolysis degree of protein and the concentration of enzymatic hydrolysate, protein and multivalent metal ions, the controllable adjustment of the performance of the surfactant can be realized; and a corresponding surfactant formula can be quickly developed according to specific application requirements.

Description

technical field [0001] The invention relates to the technical field of surfactant preparation, in particular to a biosurfactant and a preparation method thereof. Background technique [0002] Surfactants refer to substances that can significantly change the surface and interface states of a solution system, usually amphiphilic molecules with the ability to form micelles, and can be used as emulsifiers, dispersants and foaming agents. According to the source, it can usually be divided into two categories: synthetic surfactants and biosurfactants. [0003] Synthetic surfactants mainly include linear alkylbenzene sulfonates, α-olefin sulfonates, alcohol ether sulfates, alcohol sulfates, etc., which are widely used in industry, agriculture, food, cosmetics and pharmaceuticals. Common synthetic surfactants usually have an alkyl chain of ten carbons or more, resulting in poor biodegradability and bioaccumulation; the raw materials for production are mainly derived from petroleum,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01F17/30B01F17/00C09K23/30C09K23/00
Inventor 李瑞琦李华珍杨真真张晓立章家泉
Owner 百葵锐(深圳)生物科技有限公司
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