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System and method for rapidly and preliminarily screening mycoplasma in polluted milk sample

A mycoplasma and screening technology, which is applied in the field of biological culture medium preparation and biological detection, can solve the problems of high cost and labor intensity, low detection efficiency, and poor detection accuracy, so as to reduce work cost and labor intensity, improve accuracy and Efficiency and the effect of reducing inspection costs

Inactive Publication Date: 2021-10-19
大能生物科技(南京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the common methods for screening and diagnosing Mycoplasma bovis in bovine contagious mastitis mainly include colony observation, nucleic acid detection, probe, fluorescence color development and color development. Although they can meet the needs of detection operations, the detection efficiency is low and the detection The operation accuracy is poor and the cost and labor intensity of the inspection operation are relatively high

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  • System and method for rapidly and preliminarily screening mycoplasma in polluted milk sample
  • System and method for rapidly and preliminarily screening mycoplasma in polluted milk sample
  • System and method for rapidly and preliminarily screening mycoplasma in polluted milk sample

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Embodiment 1

[0036] see image 3 A screening method for rapid preliminary screening of mycoplasma in contaminated milk samples, comprising the following steps;

[0037] S1, milk pretreatment, take 1 as a milk sample, then at least one milk sample is centrifuged through centrifuge 1, and the centrifuged milk sample is filtered through filter 2 at 0.40-µm and processed The filtrate is collected for subsequent use;

[0038] S2, culture detection, adding the filtrate collected in step S1 together with the liquid medium to the culture dish 3 for culture operation, and after 20 minutes of culture, the chromaticity detection instrument 4 is used for chromogenic detection, and the detection result of mycoplasma can be obtained .

[0039] In this embodiment, the liquid medium is composed of the following components in parts by weight: 10% PPLO broth, 5% compound horse serum, 13% 25% yeast extract, 2.3% ampicillin, 2.1% vancomycin, Glucose 3%, sodium pyruvate 0.5%, bovine lung digestive juice 1.5...

Embodiment 2

[0051] see image 3 , a screening method for rapid preliminary screening of mycoplasma in contaminated milk samples, comprising the following steps;

[0052] S1, milk pretreatment, take 5ml as a milk sample, then centrifuge at least one milk sample through centrifuge 1, and filter the centrifuged milk sample through filter 2 at 0.45µm and filter the filtrate for collection;

[0053] S2, culture detection, add the filtrate collected in step S1 together with the liquid medium to the culture dish 3 for culture operation, and after 60 minutes of culture, carry out colorimetric detection by the chromaticity detector 4, and the detection result of mycoplasma can be obtained .

[0054] Wherein, the liquid medium is composed of the following components in parts by weight: 15% of PPLO broth, 11% of compound horse serum, 21% of 25% yeast extract, 4.5% of ampicillin, 3.5% of vancomycin, and 6% of glucose , sodium pyruvate 1.8%, bovine lung digestive fluid 3.1% and 1% phenol red 2.3%, ...

Embodiment 3

[0065] see image 3 , a screening method for rapid preliminary screening of mycoplasma in contaminated milk samples, comprising the following steps;

[0066] S1, milk pretreatment, take 2ml as a milk sample, then centrifuge at least one milk sample through centrifuge 1, and filter the centrifuged milk sample at 0.43µm through filter 2 and filter the filtrate for collection;

[0067] S2, culture detection, add the filtrate collected in step S1 together with the liquid medium to the culture dish 3 for culture operation, and after 30 minutes of culture, carry out chromogenic detection by the chromaticity detector 4, and the detection result of mycoplasma can be obtained .

[0068] Wherein, the liquid medium is composed of the following components in parts by weight: PPLO broth 12%, compound horse serum 8%, 25% yeast extract 15%, ampicillin 3.5%, vancomycin 2.5%, glucose 4.1% , sodium pyruvate 0.8%, bovine lung digestive juice 2.1% and 1% phenol red 1.3%, and the balance is ult...

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Abstract

The invention relates to a system and a method for rapidly and preliminarily screening mycoplasma in a polluted milk sample. The system comprises a centrifugal machine, a filter, a culture dish and a chromaticity detector, and the screening method comprises the steps of milk pretreatment and culture detection. The liquid culture medium is used for culturing the pretreated cow milk sample, and the experiment result is indicated through the chromogenic reaction, so compared with a traditional detection method, the method of the invention has the advantages that the detection efficiency is greatly improved, the detection cost is reduced, the purposes of rapidly screening pathogenic pathogens and preliminarily diagnosing infectious bovine mastitis are achieved, the working cost and the labor intensity of preliminary screening of milk in pastures or milk companies are effectively reduced, suspicious milk samples can be found in time, and the precision and the efficiency of fresh milk quality detection are improved.

Description

technical field [0001] The invention relates to a method for detecting milk mycoplasma, which belongs to the technical field of biological medium preparation and biological detection. Background technique [0002] Infectious mastitis: It is clinical or latent mastitis in dairy cows caused by infection with infectious pathogenic bacteria. It is characterized by the gradual increase in the clinical infection rate after infectious mastitis infects animals; the pathogen can be transmitted through equipment, personnel, and even feed and water sources . After Mycoplasma bovis infects first-parity dairy cows, it will lead to a decline in lactation in the later parities of infected animals, from high-yielding cows to low-yielding cows. At present, Staphylococcus aureus and Streptococcus agalactiae are the pathogens of infectious mastitis. In addition, Mycoplasma bovis (M. bovis) is also a common pathogen of bovine infectious mastitis. Mycoplasma bovis can cause local inflammation ...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12N1/20C12M1/34C12R1/35
CPCC12Q1/04C12N1/20C12M41/36
Inventor 潘子豪王凯民俞菊仙
Owner 大能生物科技(南京)有限公司
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