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Imitation extracellular matrix hydrogel dressing for diabetic foot ulcer and preparation method

A technology for diabetic foot ulcers and exogenous matrix, which can be used in pharmaceutical formulations, bandages, and drug delivery. It can solve the problems of diabetic foot ulcers, avoid toxicity and side effects, promote drug release, and keep wounds moist.

Active Publication Date: 2022-05-27
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no wound dressing for diabetic foot ulcers on the market. Based on the characteristics of impaired angiogenesis and long-term chronic inflammation of diabetic foot ulcers, a topical dressing that can significantly promote the healing of diabetic foot ulcers is designed for patients with diabetic foot ulcers. Dressings have important clinical significance and application prospects

Method used

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  • Imitation extracellular matrix hydrogel dressing for diabetic foot ulcer and preparation method
  • Imitation extracellular matrix hydrogel dressing for diabetic foot ulcer and preparation method
  • Imitation extracellular matrix hydrogel dressing for diabetic foot ulcer and preparation method

Examples

Experimental program
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Embodiment 1

[0051] The present embodiment provides a method for preparing an extracellular matrix hydrogel dressing for diabetic foot ulcers, comprising:

[0052] Step 1, preparing polydopamine nanoparticles (M@D) loaded with deferoxamine mesylate, specifically including:

[0053] Step 101: Dissolve 0.36g of surfactant Pluronic F-127 and 417μL of 1,3,5-trimethylbenzene (TMB) in 64mL of deionized water, then add 60mL of absolute ethanol, stir to mix evenly, and obtain a TMB solution; The time of described stirring can be 30min;

[0054] Step 102, adding 60 mg of dopamine hydrochloride and 90 mg of tris (hydroxymethyl) aminomethane (Tris) to the TMB solution described in step 101, stirring and reacting at room temperature for 24 h, centrifuging, washing, and lyophilizing to obtain polydopamine nanoparticles (MPDA). ); Described cleaning is to be 2:1 dehydrated alcohol and acetone mixed solution cleaning with volume ratio, and the number of times of described cleaning is 3 times;

[0055] ...

Embodiment 2

[0081] The present embodiment provides a method for preparing an extracellular matrix hydrogel dressing for diabetic foot ulcers, comprising:

[0082] Step 1, preparing polydopamine nanoparticles (M@D) loaded with deferoxamine mesylate, specifically including:

[0083] Step 101: Dissolve 0.38 g of surfactant Pluronic F-127 and 435 μL of 1,3,5-trimethylbenzene (TMB) in 64 mL of deionized water, then add 60 mL of absolute ethanol, stir to mix evenly, and obtain a TMB solution; The time of described stirring can be 30min;

[0084] Step 102, adding 65 mg of dopamine hydrochloride and 90 mg of tris (hydroxymethyl) aminomethane (Tris) to the TMB solution described in step 101, stirring and reacting at room temperature for 24 h, centrifuging, washing, and lyophilizing to obtain polydopamine nanoparticles (MPDA). ); Described cleaning is to be 2:1 dehydrated alcohol and acetone mixed solution cleaning with volume ratio, and the number of times of described cleaning is 3 times;

[00...

Embodiment 3

[0105] The present embodiment provides a method for preparing an extracellular matrix hydrogel dressing for diabetic foot ulcers, comprising:

[0106] Step 1, preparing polydopamine nanoparticles (M@D) loaded with deferoxamine mesylate, specifically including:

[0107] Step 101: Dissolve 0.40 g of surfactant Pluronic F-127 and 450 μL of 1,3,5-trimethylbenzene (TMB) in 120 mL of deionized water, then add 60 mL of absolute ethanol, stir to mix evenly, and obtain a TMB solution; The time of described stirring can be 30min;

[0108]Step 102, adding 70 mg of dopamine hydrochloride and 90 mg of tris (hydroxymethyl) aminomethane (Tris) to the TMB solution described in step 101, stirring and reacting at room temperature for 24 h, centrifuging, washing, and lyophilizing to obtain polydopamine nanoparticles (MPDA). ); Described cleaning is to be 2:1 dehydrated alcohol and acetone mixed solution cleaning with volume ratio, and the number of times of described cleaning is 3 times;

[01...

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Abstract

The invention discloses a method for preparing an extracellular matrix hydrogel dressing for diabetic foot ulcers, comprising: dispersing polydopamine nanoparticles loaded with deferoxamine mesylate in a modified human-like collagen solution , and then mixed with hyaluronic acid solution grafted with epigallocatechin gallate dimer, and added with tyrosinase solution to obtain extracellular matrix imitation hydrogel dressing. The present invention comprises dissolving polydopamine nanoparticles loaded with deferoxamine mesylate in a modified human-like collagen solution and mixing it with a hyaluronic acid solution grafted with epigallocatechin gallate dimer The imitation extracellular matrix hydrogel dressing prepared by quickly adding tyrosinase solution can specifically solve the problems of impaired angiogenesis and long-term chronic inflammation in diabetic foot ulcer wounds. It can effectively promote angiogenesis and anti-inflammation, and has high rate of wound healing.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a pseudo-extracellular matrix hydrogel dressing for diabetic foot ulcers and a preparation method. Background technique [0002] In recent years, the prevalence of diabetes in my country has shown an obvious upward trend. As a chronic disease, diabetes and its complications have gradually affected all aspects of people's lives. Diabetic foot ulcer is one of the most serious chronic complications of diabetes and the main cause of non-traumatic amputation of diabetes, which greatly reduces the quality of life of diabetic foot patients and seriously threatens the life of patients. Many studies have shown that hydrogel dressings that can keep the wound moist and absorb tissue exudate can promote wound healing, but the commercially available hydrogel dressings with the above characteristics are very limited in the treatment of diabetic foot ulcers. The reason...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L26/00
CPCA61L26/008A61L26/0019A61L26/0033A61L26/0023A61L26/0047A61L26/0066A61L26/0061A61L2400/12A61L2300/41A61L2300/412C08L79/02C08L5/08C08L89/00
Inventor 范代娣袁扬马晓轩
Owner NORTHWEST UNIV