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Method for promoting coriolopsis gallica to produce laccase by using food waste

A waste and food technology, applied in the field of microbial enzymology, can solve the problems of long fermentation cycle, low production level, high production cost, etc., and achieve the effects of promoting growth, reducing production cost, and shortening the fermentation cycle

Pending Publication Date: 2021-10-29
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for promoting the induction and expression of laccase by using food waste as the fermentation substrate to solve the problems of low production level, long fermentation period and high production cost of producing laccase by fungal fermentation , to promote the large-scale production and industrial application of fungal laccase

Method used

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  • Method for promoting coriolopsis gallica to produce laccase by using food waste
  • Method for promoting coriolopsis gallica to produce laccase by using food waste
  • Method for promoting coriolopsis gallica to produce laccase by using food waste

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Effect test

Embodiment 1

[0021] The impact of embodiment 1 different carbon sources on the production of laccase by P.

[0022] Using the commercial carbon source glucose as a control, the effects of different peel and pomace raw materials as carbon sources on the induction of laccase production by Inonotus pilosa were investigated, including the following steps:

[0023] (1) Pretreatment of food waste: drying various peel and pomace waste raw materials at 60°C until the water content is lower than 10%, crushing, passing through a 60-mesh sieve, and sealing and storing;

[0024] (2) Preparation of seed solution: pick a ring of hyphae from the preserved slant of P. pilosa and insert it into a sterile 150mL Erlenmeyer flask equipped with 50mL liquid PDA medium and a magnetic stirrer, at 28°C, Shake the flask at 120rpm until the bottle is covered with mycelial balls, use magnetic stirring to break up the mycelial balls, pour the bacterial liquid into a sterile 50mL centrifuge tube, centrifuge at 10000rpm...

Embodiment 2

[0028] The influence of embodiment 2 different nitrogen sources on the laccase production of Inonotus pilosa

[0029] Adopt the method of embodiment 1, preferably pomelo peel powder is carbon source, design is nitrogen source with bran powder, rapeseed meal powder, peptone, yeast extract powder, ammonium sulfate, ammonium tartrate, investigates the nitrogen source of different sources to coarse hair leather The effect of laccase production by Poriobacterium.

[0030] The result is as figure 2 The results show that bran powder has the best induction effect on the laccase produced by the fermentation of Inonotus roughae, and the maximum laccase activity was obtained after 5 days of fermentation, reaching 5865U / L, which was significantly higher than that of other treatment groups, followed by rapeseed meal powder, The enzyme activities of the other commercial nitrogen source groups were all low. The induction effects of bran powder and rapeseed meal powder were significantly h...

Embodiment 3

[0031] The influence of embodiment 3 different carbon-nitrogen ratios produce laccase by P.

[0032] Using the method of Example 1, the pomelo peel powder was preferably used as the carbon source, and the bran powder was used as the nitrogen source to investigate the effects of different carbon-to-nitrogen ratios on the production of laccase by P.

[0033] According to JY / T 017-1996 method, adopt elemental analyzer to measure the total carbon of pomelo peel powder and bran powder, total nitrogen content (result is shown in table 1), adjust the addition amount of pomelo peel powder and bran powder, make The carbon-nitrogen ratios of the mixed materials are 12:1, 16:1, 20:1, 24:1, 28:1, 32:1 respectively.

[0034] Table 1 The total carbon and total nitrogen components of pomelo peel powder and bran powder

[0035] Total Carbon (%) Total nitrogen (%) carbon to nitrogen ratio pomelo peel powder 41.35 1.00 41.35 bran powder 40.37 3.30 12.23

[...

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Abstract

The invention discloses a method for inducing coriolopsis gallica to express laccase by using food waste. According to the method, the food waste is used as a fermentation substrate, provides a carbon source, a nitrogen source and a biological induction factor for the coriolopsis gallica, and promotes mycelial growth and laccase induced expression; and the method mainly comprises the following steps: (1) pretreatment of food processing waste; (2) preparation of a coriolopsis gallica seed solution; (3) preparation of a liquid enzyme production culture medium; and (4) production of the laccase through liquid fermentation. The method for inducing the coriolopsis gallica to express the laccase by using the food waste, which is provided by the invention, is high in enzyme production efficiency, short in fermentation period and low in cost, does not need to additionally add any chemical inducer, meanwhile, is beneficial for promoting the resource utilization of the food waste, and has obvious economic benefits and social benefits.

Description

technical field [0001] The invention belongs to the technical field of microbial enzymology, and in particular relates to a method for promoting the production of laccase by Inonotus gracilis by using food waste. Background technique [0002] White-rot fungi are one of the main sources of laccase, and there are two types of laccase secreted by strains: constitutive and inducible. The expression of fungal laccase is related to many factors such as fermentation conditions and induction conditions, so the production of laccase can be improved by optimizing the medium composition and culture conditions. In the process of fungal fermentation, adding an appropriate amount of inducer has a significant effect on increasing the expression of laccase. Common chemical inducers include copper sulfate, aromatic inducers such as guaiacol, 2,6-dimethylphenol, and p-hydroxybenzaldehyde, but most of these inducers are environmental pollutants and have certain toxicity. It does not conform ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/02C12R1/645
CPCC12N9/0061C12N1/14C12Y110/03002
Inventor 巫小丹岑庆静卢艳娟崔宪刘玉环徐立娜付桂明洪妮李绪万辉刘薇
Owner NANCHANG UNIV
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