Saccharomyces cerevisiae SG35, fermentation inoculant containing saccharomyces cerevisiae SG35 and application of saccharomyces cerevisiae SG35 or fermentation inoculant
A technology of Saccharomyces cerevisiae and fermentation agent, which is applied in the field of food processing, can solve the problems of high alcohol content, affecting the taste and aroma of wine, etc., and achieve the effect of low alcohol output, low output of bad flavor substances, and thorough fermentation
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Embodiment 1
[0044] This example provides the isolation, purification and identification of Saccharomyces cerevisiae SG35 strain
[0045] The unsterilized Syrah grapes are crushed and put into the fermentation vessel with the skins, and fermented at a constant temperature of 25°C. Detect the content of reducing sugar in the fermentation process, take samples according to the consumption of reducing sugar in different fermentation stages (sugar consumption 20%, 50%, 80%), draw the grape juice of different fermentation stages and use sterile physiological saline to carry out gradient dilution ( 10 -5 、10 -6 、10 -7 ) and spread it on the WL nutrient agar medium plate, after culturing at 30°C for 24 hours, pick a single colony with good growth and obvious morphological characteristics from the plate, and carry out the colony morphology and molecular biology identification (5.8S-rDNA ITS method) , a total of 89 strains of Saccharomyces cerevisiae were obtained. Then 8 strains of S. cerevisi...
Embodiment 2
[0057] This example provides the tolerance evaluation of Saccharomyces cerevisiae SG35
[0058] A single colony of Saccharomyces cerevisiae SG35 was inoculated in a 96-well plate containing sterile YPD liquid medium, cultured overnight at 30°C and 200 rpm for activation, and then the following tolerance experiments were performed respectively.
[0059] 1) Ethanol concentration tolerance test
[0060] The activated bacterial strain in the above-mentioned 96-well plate was transferred to the YPD liquid medium containing 6%, 8%, 10%, 12%, 14%, 16%, and 18% ethanol volume concentration respectively with an inoculation amount of 3%, After culturing overnight at 30°C and 200 rpm, measure the OD with a microplate reader 600nm value, draw the ethanol concentration tolerance curve of Saccharomyces cerevisiae, such as image 3 shown.
[0061] 2) Glucose concentration tolerance test
[0062] The activated bacterial strains in the above-mentioned 96-well plate were transferred to YPD ...
experiment example 1
[0072] Saccharomyces cerevisiae SG35 and commercial yeast RX60 (produced by Laffort, France, commonly used in dry red wine brewing) were inoculated into grape juice simulated medium respectively, and a small-scale fermentation experiment was carried out. At the same time, the growth curve and sugar consumption during the fermentation process were monitored. Compare fermentation capacity. After the fermentation, the content of ethanol and the content of volatile aroma substances in the fermented wine were detected. The specific results are shown in Table 1-2 and attached Figure 7-8 .
[0073] The specific method of small test fermentation experiment is as follows:
[0074] Saccharomyces cerevisiae SG35 (obtained by screening according to the method in Example 1) and commercial Saccharomyces cerevisiae RX60 stored at -80°C were respectively passed on YPD solid and YPD liquid medium, and strains were picked from the YPD plate and inserted into the In a 100 mL Erlenmeyer flask...
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