Unlock instant, AI-driven research and patent intelligence for your innovation.

Construction method of specific chromatogram of gypsum standard decoction

The technology of a characteristic map and a construction method is applied in the field of construction of the characteristic map of gypsum standard decoction, and can solve the problem of high price of X-ray diffractometer

Pending Publication Date: 2021-11-12
GUANGDONG YIFANG PHARMA +1
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Traditionally, the construction of gypsum fingerprints mainly uses X-ray diffraction analysis, but the price of X-ray diffractometer is relatively high, and X-ray diffraction analysis is mainly aimed at the unit cells that make up mineral medicines, not the elements in them, so the established fingerprints are only Can target the most main part, while other distinctive parts (such as inorganic elements) will be ignored

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Construction method of specific chromatogram of gypsum standard decoction
  • Construction method of specific chromatogram of gypsum standard decoction
  • Construction method of specific chromatogram of gypsum standard decoction

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0062] The preparation of the test solution includes: using an extraction solvent to extract the gypsum standard decoction, and collecting the extract to obtain the test solution; the extraction solvent is methanesulfonic acid solution.

[0063] The "Chinese medicine chromatographic fingerprint similarity evaluation system" described in the present invention is a software issued by the National Pharmacopoeia Committee that can compare the similarity of the spectra.

[0064] The "extraction solvent" mentioned in the present invention is the "extraction acid" mentioned in the specific examples. Unless otherwise specified, the concentration of the extraction acid is expressed in mass percentage.

[0065] It should be noted that, in the construction method of the present invention, there is no chronological order limitation for the steps involved.

[0066] The mass percentage of methanesulfonic acid in the extraction solvent can be selected from, including but not limited to, any ...

Embodiment 1

[0095] Embodiment 1, establishment of feature map analysis method

[0096] 1. Instruments, reagents and reagents

[0097] 1.1 Instruments: ion chromatograph (Dionex ICS-6000, Thermo Fisher Scientific Co., Ltd.); cation suppressor (CSRS300 4mm, Thermo Fisher Scientific Co., Ltd.); IonPac CG12A guard column (4×50mm, 8μm) ; IonPacCS12A analytical column (4 × 250mm, 8μm); ten-thousandth analytical balance (ME204E, Mettler-Toledo); ultrapure water system (Milli-Q Direct, Merck AG).

[0098] 1.2 Reagents: hydrochloric acid (Guangzhou Chemical Reagent Factory, superior grade); Sulfuric acid (Guangzhou Chemical Reagent Factory, superior grade); Methanesulfonic acid (MSA, Aladdin Reagent (Shanghai) Co., Ltd., 99.5%); water is ultrapure Water (self-made in the laboratory).

[0099] 1.3 Reagent: standard substance for sodium component analysis in water (first-level metering station of applied chemistry for national defense science and technology industry, concentration: 1000μg / mL, ba...

Embodiment 2

[0163] Embodiment 2, methodological verification of characteristic map

[0164] (1) Specificity inspection

[0165] With reference to the detection conditions determined in Example 1 and the preparation method of the test solution, precision draw gypsum standard decoction (GT161022) to prepare each 5 μl of the test solution, reference solution and blank solvent (for 0.54% methanesulfonic acid), inject ion chromatograph for determination. see results Figure 16 .

[0166] Table 7. Chromatographic peak separation parameter list

[0167]

[0168] The results show that need testing solution has the same chromatographic peak at the corresponding retention time of the reference substance chromatogram, although the blank solvent also has Na + 、K + Chromatographic peaks, but the peak areas are only 2.54% and 4.42% of the sample, which has little effect on the determination of the sample, indicating that the specificity of the method is good.

[0169] (2) Inspection of preci...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
particle diameteraaaaaaaaaa
lengthaaaaaaaaaa
Login to View More

Abstract

The invention relates to a construction method of a specific chromatogram of a gypsum standard decoction, which comprises the following steps: providing a reference solution, preparing a test solution, detecting the test solution and the reference solution by ion chromatography, importing a chromatogram obtained by detecting the reference substance solution and a chromatogram obtained by detecting the test solution into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system, and formulating the specific chromatogram of the gypsum standard decoction; the reference substance solution is a solution obtained by dissolving a reference substance in a solvent, and the reference substance comprises sodium ions, potassium ions and magnesium ions; the preparation of the test solution comprises the following steps: extracting the gypsum standard decoction by adopting an extraction solvent, and collecting an extracting solution to obtain the test solution, and the extraction solvent is a methanesulfonic acid solution. The specific chromatogram of the gypsum standard decoction is constructed for the first time. In the specific chromatogram, the relative retention time of each characteristic peak is stable, and the relative peak area is obviously different from the whole relative peak area of other mineral drugs, so that specificity is shown.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine detection, in particular to a method for constructing a characteristic map of a gypsum standard decoction. Background technique [0002] Gypsum, as hydrous calcium sulfate (CaSO 4 2H 2 O) ore, aliased as fine stone, fine marble ("Do not record"), soft gypsum ("Supplement to Materia Medica Yanyi"), cold water stone ("Compendium"), Baihu ("Drug Chemicals"). Gypsum is a monoclinic mineral, often produced in the sedimentary rocks formed by gulf salt lakes and inland lakes. It is translucent, heavy, soft, with a silk-like luster on the longitudinal section, with a slight smell and a light taste. [0003] Gypsum has the following pharmacological effects: (1) Antipyretic effect: Studies in recent years have shown that single gypsum and Baihu decoction have certain antipyretic effects on experimentally induced fever rabbits. Natural gypsum 1:1 decoction, 4mL administered rectally ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8679Y02A50/30
Inventor 李国卫黄跃前吴文平钟春琳刘晓琳杨晓东程学仁陈向东邱韵静黄上书
Owner GUANGDONG YIFANG PHARMA