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Application of sanguinarine in preparation of canavalia urease inhibitor

The technology of sanguinarine and bean urease is applied to the application field of sanguinarine in the preparation of urease inhibitor of canava, and can solve the problems of poor stability, short action time, obvious toxic and side effects, etc. The effect of enriching optional types and good development prospects

Active Publication Date: 2022-06-07
遵义医科大学珠海校区
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the existing urease inhibitors have problems such as poor stability, short action time, and obvious toxic and side effects, which have certain limitations in practical application.

Method used

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  • Application of sanguinarine in preparation of canavalia urease inhibitor
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  • Application of sanguinarine in preparation of canavalia urease inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Study on the inhibitory effect of sanguinarine on urease

[0035] Mix the concanava urease solution with a series of concentrations of the test drug solution, and incubate at 37°C for 20 minutes; add urea solution, and react in the dark for 20 minutes at room temperature; add Berthelot chromogenic solution for 10 minutes in the dark, Pipette 200 μL of the incubation solution onto a 96-well plate, and measure the OD at 595 nm on a microplate reader 绝对 value. Each concentration was done 3 times in parallel. The blank sample was replaced by the solvent of each dilution, and the rest of the operations were the same as above, and the OD was measured. 空白 . According to formula 1, the corresponding OD is obtained 相对 value. The residual enzyme activity (RA) was obtained according to formula 2, and the corresponding half inhibitory concentration IC was obtained by GraphPad 50 .

[0036] OD 相对 =OD 绝对 –OD 空白 (Formula 1)

[0037] Residual enzyme activity (%) ...

Embodiment 2

[0039] Example 2: Study on the type of inhibition of sanguinarine on urease

[0040] Take a certain concentration of concanava urease and a series of concentrations of sanguinarine solution (0, 0.063, 0.125, 0.25mM), mix well, and incubate at 37°C for 20 minutes. Then, a series of concentrations of urea solution (0.468-7.5mM) were added at room temperature to react in the dark for 20 minutes, and then the color was developed according to the method of 2.1 and the OD was measured. 绝对 value and obtain the corresponding OD 相对 value. Taking the solvent added to the substance as a blank control, the determination was performed in parallel for 3 times. The experiment passes the reciprocal of the reaction speed (1 / V, ie 1 / OD 相对 ) plot the inverse of the substrate concentration (1 / [urea]) as a Lineweaver-Burk diagram, and finally obtain the kinetic parameter K by combining the L-B curve with Equation 3 M , v max , and the inhibition constant K is obtained by quadratic plotting of...

Embodiment 3

[0043] Example 3: Influence of sulfhydryl-containing compounds on urease activity of concanava

[0044] 1. Incubation time test

[0045] Mix the concanava urease solution, sulfhydryl-containing compound solution (DTT solution, L-Cys solution or GSH solution, all at a concentration of 1.25mM) and 0.25mM sanguinarine solution, and pre-incubate at 37°C for 5, 10, and 20 minutes, respectively. Or after 40min, add urea at room temperature to react for 20min, then develop color according to the method in Example 1 and measure OD 绝对 value and obtain the corresponding OD 相对 value, and obtain the corresponding residual enzyme activity RA. Taking the solvent added to the substance as a blank control, the determination was performed in parallel for 3 times.

[0046] 2. Interaction test of sanguinarine-thiol-urease

[0047] Mix the two of the urease solution, the solution containing sulfhydryl compounds (DTT solution, L-Cys solution or GSH solution, all at 1.25 mM) and 0.25 mM sanguin...

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Abstract

The invention belongs to the technical field of application of natural compounds, and discloses the application of sanguinarine in the preparation of canavalia urease inhibitors. The invention points out that the sanguinarine has remarkable effect of inhibiting the urease of canavalia, has the characteristics of simple structure, good safety and the like, and has good application value and development prospect in the field of plant fertilizer or animal feed additive.

Description

technical field [0001] The invention belongs to the technical field of application of natural compounds, and in particular relates to the application of sanguinarine in the preparation of urease inhibitor of concanavalina. Background technique [0002] Urease, also known as urease or urea amide hydrolase, has a molecular weight of about 120,000-130,000. The concanava urease in urease is the first metal enzyme found to contain nickel ions. It is a milestone in the history of biochemical development. Urease is widely present in Various bacteria, fungi, actinomycetes and plants decompose urea into carbon dioxide compounds and ammonia. Urease helps plants and microorganisms use endogenous and exogenous urea as a nitrogen source, and can synthesize the ammonia produced by decomposition into body proteins. However, the existence of urease also accelerates the process of urea decomposition to release ammonia gas, which greatly reduces the utilization rate of nitrogen by animals an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/4741C05G3/90A23K20/137
CPCC05G3/90A23K20/137Y02A50/30
Inventor 李彩兰鲁强陈雨佳
Owner 遵义医科大学珠海校区
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