Bioactive substance composition, serum-free culture medium containing composition and application of serum-free culture medium

A technology of biologically active substances and compositions, applied in the field of biomedicine, can solve the problems of slow cell proliferation, failure of damage repair, and small number, and achieve the effects of good cell growth, clear components and unique proportions

Pending Publication Date: 2021-11-23
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0028] Aiming at the existing in vitro cell culture problems such as slow cell proliferation, small number, easy loss of phenotype, unstable cell quality, poor safety, and damage repair failure after transplantation, the present invention provides a bioactive substance composition, Serum-free medium comprising said composition and use thereof

Method used

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  • Bioactive substance composition, serum-free culture medium containing composition and application of serum-free culture medium
  • Bioactive substance composition, serum-free culture medium containing composition and application of serum-free culture medium
  • Bioactive substance composition, serum-free culture medium containing composition and application of serum-free culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0178] In this example, two groups of medium were prepared, namely: serum-free medium and serum control medium, and the cultured cells were tendon stem cells (hTSPCs) extracted from human normal tendon tissue. The following are detailed experiments and tests step:

[0179] Medium configuration

[0180] Serum-free medium (SFM)

[0181] The serum-free medium includes basal medium and additional components; the basal medium is selected from DMEM / F12 medium, and 5mmol of HEPES, 10000U of penicillin and 10000U of streptavidin are added in every 500mL of DMEM / F12 medium and add additional components so that the concentration range ratio of each component in the serum-free medium is: fibroblast growth factor: platelet-derived growth factor: transforming growth factor-β: glucocorticoid: heparin or its salt: Vitamin C or its derivatives: transferrin: insulin: progesterone: putrescine or its salts: selenite: epidermal growth factor: CHIR99021=10:10:5:2:1000:25000:2000:500:2 :500:2:10...

Embodiment 2

[0259] In this example, two groups of medium were prepared, namely: serum-free medium and serum control medium, and the cultured cells were ligament stem cells obtained by separating and culturing human ligament tissue. The following are the detailed experimental and detection steps:

[0260] Medium configuration

[0261] Serum-free medium (SFM)

[0262] The serum-free medium includes basal medium and additional components; the basal medium is selected from F10 medium, and 5mmol of HEPES, 10000U of penicillin and 10000U of streptomycin are added to each 500mL of F10 medium, and Add components so that the concentration range ratio of each component in the serum-free medium is: fibroblast growth factor: platelet-derived growth factor: transforming growth factor-β: glucocorticoid: heparin or its salt: vitamin C or its Derivatives: transferrin: insulin: progesterone: putrescine or its salt: selenite=15:10:6:2:1500:25000:75000:3000:4:7:4.

[0263] Further, the concentration of ea...

Embodiment 3

[0276] In this example, two groups of medium were prepared, namely: serum-free medium and serum control medium, and the cultured cells were tendon stem cells (hTSPCs) extracted from human normal tendon tissue. The following are detailed experiments and tests step:

[0277] Medium configuration

[0278] Serum-free medium (SFM)

[0279] The serum-free medium includes basal medium and additional components; the basal medium is selected from DMEM / F12 medium, and 5mmol of HEPES, 10000U of penicillin and 10000U of streptavidin are added in every 500mL of DMEM / F12 medium element, and add additional components so that the concentration of the additional components in the serum-free medium is: fibroblast growth factor: platelet-derived growth factor: transforming growth factor-β: glucocorticoid: heparin or its salt: vitamin C or its derivatives: transferrin: insulin: progesterone: putrescine or its salt: selenite=50:50:40:11:1000:100000:300000:25000:25:25000:25; The medium also cont...

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Abstract

The invention provides a bioactive substance composition, a serum-free culture medium containing the composition and application of the serum-free culture medium. The bioactive substance composition is used for a serum-free culture medium and/or a composition and preparation thereof; the serum-free culture medium and/or composition can be used for primary culture and subculture of cells and/or tissues. The cells are selected from any one or more of tendon and/or ligament source cells, chondrocytes, meniscus stem cells, mesenchymal stem cells, skeletal stem cells and muscle stem cells. The tissue is a source tissue of a motor system. The bioactive substance composition and/or the serum-free culture medium and/or the composition can be used for preparing a medicine for treating tissue and/or organ injury; the tissue or organ injury is selected from movement system tissue or organ injury.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a serum-free medium prepared from biologically active substances and its application. Background technique [0002] According to the World Health Organization data, tissue and organ damage is the second leading cause of disability and an important health problem. Due to the rapid development of industry, agriculture, transportation and sports, the trauma caused by various accidents is increasing day by day. In 2008, the data released by the National Bureau of Statistics showed that trauma and poisoning were the fifth leading cause of death. With the development of social economy and the acceleration of transportation and urban construction, the human body caused by sports injuries, traffic accidents, natural disasters (such as Wenchuan and Yushu earthquakes), industrial accidents (such as Tianjin Port explosion accident, Fujian Zhangzhou PX factory explosion), etc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/00C12N5/00A61P19/00A61P21/00
CPCC12N5/066C12N2513/00C12N2533/52C12N2500/25C12N2501/115C12N2501/135C12N2501/11C12N2501/15C12N2501/727C12N2500/99C12N2501/39C12N2501/392C12N2500/46C12N2501/105C12N5/0037C12N2500/32C12N5/0667C12N2501/91C12N2500/24C12N2501/33C12N2500/38C12N2501/999C12N2500/90C12N5/0662
Inventor 陈晓张鸿欧阳宏伟茵梓沈炜亮
Owner ZHEJIANG UNIV
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