Method for improving bile salt tolerance of lactobacillus and application thereof

A technology of Lactobacillus and Lactobacillus plantarum, applied in the field of food science, can solve the problems of changing cell surface hydrophobicity and potential, destroying bacterial permeability and fluidity, protein misfolding and denaturation, etc., achieving good application prospects and low cost Low, the effect of solving the negative impact

Pending Publication Date: 2021-11-26
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the physical and chemical properties of its detergent, it is highly toxic to intestinal microorganisms, can destroy the permeability and fluidity of bacteria, change the hydrophobicity and electric potential of cell surfaces, and even cause DNA damage, induce protein misfolding and Transgender, etc.

Method used

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  • Method for improving bile salt tolerance of lactobacillus and application thereof
  • Method for improving bile salt tolerance of lactobacillus and application thereof
  • Method for improving bile salt tolerance of lactobacillus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0036] Example 1: Different concentrations of L-malic acid improve the effect of Lactobacillus paracasei L9 on the resistance of mixed bile salts

[0037] The growth medium (CDM) used in this example includes: 200mL 100mM potassium phosphate buffer, 1g sodium acetate, 0.6g triammonium citrate, 0.5g ascorbic acid, 10g glucose, MgSO 4 0.5g, MnSO 4 0.25g, 10000×ZnSO 4 Add 100 μL of stock solution, 10 mL of 100×vitamin stock solution, 10 mL of 100× nucleic acid stock solution, 10 g of tryptone, 1 mL of Tween-80, 1 mL of glycerin, add 800 mL of distilled water, adjust the pH to 6.8, and make up to 1 L.

[0038] Lactobacillus paracasei L9 was inoculated in the CDM medium at 2%, and mixed bile salts were added at a concentration of 0%-0.2% (w / v, g / mL, the same below) to the medium, and stood at 37°C Incubate, measure OD every two hours 600The value of the growth curve was measured to compare the inhibitory effect of different concentrations of mixed bile salts on the growth of L...

example 2

[0040] Example 2: The impact of different concentrations of L-malic acid improving Lactobacillus paracasei L9 on the resistance of single bile salt glycine deoxycholate (GDCA)

[0041] The growth medium used in this example is the same as Example 1. Lactobacillus paracasei L9 was inoculated in CDM medium at 2%, and single bile salt GDCA bile salt was added to the medium at a concentration of 0%, 0.04%, 0.06%, 0.08%, 0.1% and 0.12%, at 37°C Static culture, measure OD every two hours 600nm value. The result is as image 3 As shown, the concentration of 0.08% GDCA was selected to evaluate the effect of different concentrations of L-malic acid on improving the resistance of L9 to single bile salt GDCA.

[0042] Then Lactobacillus paracasei L9 was inoculated at 2% in the CDM medium containing 0.08% GDCA while adding different concentrations of L-malic acid to adjust the pH to 6.8. It can be found from the measurement results of the growth curve, such as Figure 4 It is shown t...

example 3

[0043] Example 3: L-malic acid is to the assay result of improving the bile salt tolerance of various lactic acid bacteria

[0044] The CDM medium used in this example is the same as Example 1. Use CDM medium to continuously activate 11 different strains of Lactobacillus for 2-3 generations, and inoculate the activated strains with 2% inoculum to 0.2% mixed bile salts with L-malic acid content of 0g / L and 5g / L respectively In the CDM medium, adjust the pH to 6.8, culture statically at 37°C, and measure the OD for 14 hours 600nm The absorbance was compared with the absorbance grown in CDM without adding bile salts, and the ratio of absorbance between bile salts treated and untreated was used as an index to evaluate the salt tolerance of different lactobacilli.

[0045] Such as Figure 5 As shown, compared with adding 0.2% mixed bile salt group, adding L-malic acid to bile salt can significantly improve the bile salt tolerance of 11 strains of lactic acid bacteria, including L...

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Abstract

The invention provides a method for improving bile salt tolerance of lactobacillus and application thereof. The method comprises the step of: adding L-malic acid into a growth culture medium of lactic acid bacteria. According to the method, the bile salt tolerance of the lactobacillus can be remarkably improved, the growth performance of lactobacillus itself cannot be affected, and the viable count of the probiotics in intestinal tracts can be increased. The method disclosed by the invention is simple to operate, low in cost and high in practicability, has general adaptability to various commercially available microecologics and potential functional probiotics, and has a good application prospect in the field of food industry.

Description

technical field [0001] The invention relates to the technical field of food science, in particular to a method and application for improving the bile salt tolerance of lactobacillus. Background technique [0002] The World Health Organization (WHO) and the Food and Agriculture Organization (FAO) jointly define probiotics as "live microorganisms that, when ingested in sufficient quantities, confer certain health benefits on the body". Some lactobacilli, such as Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus casei, are recognized as probiotics. Probiotics have a variety of beneficial functions. By colonizing in the human body, they can change the flora composition of a certain part of the host, regulate the immune function of the host mucosa and system, participate in the metabolism of carbohydrates and fats in the host, and reduce gastrointestinal diseases and metabolic diseases. and other functions such as the occurrence of chronic diseases. The prere...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/38A23L33/135A23L27/20A23L3/3508A23L5/41A23K20/105C12R1/225C12R1/25C12R1/245C12R1/23
CPCC12N1/20C12N1/38A23L33/135A23L27/2028A23L3/3508A23L5/41A23K20/105A23V2002/00A23V2400/137A23V2400/165A23V2400/125A23V2400/175A23V2400/113A23V2400/169A23V2200/3204
Inventor 郝彦玲宦冉翟征远
Owner CHINA AGRI UNIV
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