Low-gossypol high-nutrition fermented cottonseed meal, preparation method and application

A cottonseed meal, high nutrition technology, applied in application, food processing, animal husbandry and other directions, can solve the problems of high production cost, large investment, low degradation efficiency of gossypol, etc., and achieve the effect of improving nutritional performance and low cost

Active Publication Date: 2022-07-01
播恩集团股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are existing methods for dephenolizing cottonseed meal, such as preparing fermented cottonseed meal by solid fermentation of Bacillus subtilis, Aspergillus niger, Aspergillus oryzae, and lactic acid bacteria. The detoxification rate of free gossypol in this method is 46% to 82%. The degradation efficiency of gossypol is not high, and there is a problem of insufficient amino acid balance of the product
[0005] In summary, the existing technology is difficult to balance the elimination of anti-nutritional factors, the improvement of cottonseed meal protein quality, and low material loss, and the investment is large and the production cost is high.

Method used

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  • Low-gossypol high-nutrition fermented cottonseed meal, preparation method and application
  • Low-gossypol high-nutrition fermented cottonseed meal, preparation method and application
  • Low-gossypol high-nutrition fermented cottonseed meal, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The present embodiment provides a preparation method of fermented cottonseed meal with low gossypol and high nutrition, comprising the following steps:

[0038] S1: Preparation of Aspergillus oryzae seeds

[0039] Mix 70% of cottonseed meal, 15% of bran, and 15% of corn flour to form a mixture, add Aspergillus oryzae spore suspension to the mixture according to a solid-liquid ratio of 10:7, and put it into a shallow dish after mixing evenly (the depth of the shallow dish is 10:7). 25~30cm), cultured at 28°C for 3 days to prepare rice koji, the number of Aspergillus oryzae spores in the rice koji is ≥1×10 8 cuf / g, neutral protease activity ≥5000u / g.

[0040] S2: Add rice koji with a weight percentage of 6% to the cottonseed meal, and place it in a double-shaft mixer for 90s. After mixing, the material enters the feed bin through a hoist, and the material is crushed by an ultrafine pulverizer for use. The particle size of the superfinely pulverized mixture must meet the...

Embodiment 2

[0066] The purpose of this example is to explore the effect of different carbon sources on the proliferation of Candida tropicalis and Lactobacillus rhamnosus complex bacteria. The specific experimental methods are as follows.

[0067] Referring to Example 1, a method for preparing fermented cottonseed meal with low gossypol and high nutrition, steps S1, S2, and S3 are the same as those in Example 1, and the carbon source soybean molasses in step S4 is respectively sucrose, glucose, sucrose molasses, and corn flour. , soluble starch is replaced by the same amount, and other ingredients remain unchanged. Adjust the PH of the primary fermentation broth to be 4.0-5.5, set the temperature to 35°C, the stirring speed to be 120 r / min, and the ventilation rate to be 0.25 vvm, continue to aerate and stir for 30 hours to carry out secondary aerobic fermentation to obtain secondary fermentation broth. The number of Candida tropicalis and Lactobacillus rhamnosus in the fermentation broth...

Embodiment 3

[0073] The purpose of this example is to explore the effect of the low-gossypol and high-nutrient fermented cottonseed meal obtained in Example 1 on the production performance of finishing pigs when the low-protein diet is applied. The specific test method is as follows.

[0074] 1 Materials and methods

[0075] 1.1 Experimental Design

[0076] For the experiment, 40 healthy Du × Chang × Da ternary hybrid growing pigs with an initial weight of about 72 kg were selected and randomly divided into 2 groups, with 20 replicates in each group, and 1 pig in each replicate. The basal diet was formulated according to NRC (2012) for the conventional protein group. The low-protein group replaced soybean meal with fermented cottonseed meal in Example 1. The protein content was reduced by 2 percentage points on the basis of the conventional histone protein group, and synthetic amino acids were supplemented. Net energy levels and standard ileal digestible amino acid levels remained basical...

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Abstract

The invention belongs to the field of fermentation engineering and livestock and poultry feed, and particularly relates to low-gossypol and high-nutrition fermented cottonseed meal as well as a preparation method and application thereof.The low-gossypol and high-nutrition fermented cottonseed meal is prepared by performing superfine grinding on rice koji and cottonseed meal in advance, then performing aerobic fermentation for the first time, and then adding compound enzyme and compound bacteria into fermentation liquor for fermentation for the second time. After two times of fermentation, gossypol in the cottonseed meal is fully and effectively degraded, macromolecular nutritional ingredients such as crude protein, crude fiber and non-starch polysaccharide in the cottonseed meal are also effectively degraded, and the application performance of the fermented cottonseed meal as livestock and poultry feed is comprehensively improved. When the fermented cottonseed meal is applied to low-protein daily ration, the dependence of the daily ration on the raw material soybean meal can be effectively changed, and meanwhile, the dosage of formula protein is reduced.

Description

technical field [0001] The invention belongs to the field of fermentation engineering and livestock and poultry feed, and particularly relates to a fermented cottonseed meal with low gossypol and high nutrition, a preparation method and application. Background technique [0002] Cottonseed meal is a by-product of cottonseed after hulling, linting, and oil extraction. It is a commonly used feed protein raw material in animal husbandry. The crude protein content of cottonseed meal is 35% to 50%. At present, most of the feeds added with cottonseed meal are fed to ruminants, and the amount added to non-ruminant animal feeds such as pigs and chickens is relatively small, and the maximum addition amount is 5% to 10%. The reasons for such a low addition amount are as follows. Two aspects: (1) cottonseed meal contains anti-nutritional factors such as gossypol, cyclopropene fatty acid, tannin, especially gossypol, which can easily cause animal poisoning and even death; (2) the amino ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/37A23K10/14A23K20/189A23K50/30
CPCA23K10/12A23K10/37A23K10/14A23K20/189A23K50/30A23V2400/175Y02P60/87
Inventor 李红胜倪冬姣邹新华许赣荣黄明媛邢宏博宋敏宋汉良向江波邢孔萍
Owner 播恩集团股份有限公司
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