Inorganic salt-protein composite medical instrument
A technology of medical devices and inorganic salts, applied in the field of medical devices, can solve problems such as adverse effects of protein functions and difficulties in obtaining expected results, and achieve the effects of avoiding aseptic manufacturing methods, inhibiting inactivation, and reducing costs
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example 1
[0198] Example 1: Cell Proliferation Activity After Radiation Sterilization of External Fixation Pins Coated with FGF-2-Encapsulated Apatite
[0199] Titanium internal fixation pins used in fracture fixation were coated with an inorganic salt solid embedded with FGF-2 having cell proliferation activity, and the whole was sterilized by ionizing radiation to examine whether FGF-2 had cell proliferation activity.
[0200] Contains 4.89mM Ca ions, 1.28mM Phosphate ions, 6.13mM K ions, 138.8mM Na ions, 0.23mM Mg ions, 136.6mM Cl ions, 15.09mM HCO 3 An unstable calcium phosphate supersaturated solution that is ionic and has a pH of 7.8, and can crystallize calcium phosphate by spontaneous nucleation in about 4-5 hours even if it is placed directly at 37°C (the unstable calcium phosphate supersaturated solution is related to the patent document 19 different fluids). Fibroblast growth factor-2 (FGF-2) was added to the unstable calcium phosphate supersaturated solution at concentratio...
example 2
[0206] Example 2: Cell Proliferation Activity After Radiation Sterilization of External Fixation Pins Coated with FGF-2 Adsorbed Apatite
[0207] Titanium internal fixation pins used in fracture fixation were coated with an inorganic salt solid adsorbed with FGF-2 having cell proliferation activity, and the whole was sterilized by ionizing radiation to examine whether FGF-2 had cell proliferation activity.
[0208] Using the same unstable calcium phosphate supersaturated solution as in Example 1, a supersaturated calcium phosphate solution without FGF-2 was prepared, and 6 or 8 titanium internal fixation pins (Synthes, Inc. Cell Drill 4.0 / 3.0mmTi, 20mm-80mm) was dipped in it for 48 hours to make Ap pins coated with apatite on the surface. This Ap pin was dipped in supersaturated calcium phosphate containing 12 μg / ml FGF-2 for a few seconds, frozen at -18°C, and adsorbed apatite FGF-2 coated with FGF-2-adsorbed apatite ( Adsorbs ApFGF) pins. Under exactly the same conditions...
example 3
[0213] Example 3: Cell Proliferation Activity After Radiation Sterilization of External Fixation Pins Coated with FGF-2-Encapsulated Gelatin
[0214]Dip the same titanium internal fixation pin as in Example 1 for a few seconds in a 1% gelatin solution containing 4 μg / ml of FGF-2, freeze at -18°C, and prepare a pin coated with FGF-2-embedded gelatin (Gelatin FGF). Under exactly the same conditions as in Example 1, vacuum drying, γ-ray irradiation or non-irradiation, storage, and evaluation of cell growth activity were performed at room temperature. The operation of applying FGF-2-embedded gelatin, irradiating or not irradiating γ rays, and measuring the proliferation rate was repeated four times.
[0215] Table 3 shows the number of gelatin FGF pins judged to be "active" in 4 replicates. also, image 3 The value of the measured proliferation rate is shown.
[0216] [table 3]
[0217]
[0218] As shown in Table 3, in 4 experiments, 13 / 13 gamma-ray irradiated group and 13...
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