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Stem cell factor freeze-dried powder capable of reducing triglyceride as well as preparation method and application of stem cell factor freeze-dried powder

A stem cell factor and triglyceride technology, applied in the field of biomedicine, can solve the problems of missed treatment and prevention time, non-sensitivity, etc., to reduce the first-pass effect, improve bioavailability, and improve insulin resistance.

Pending Publication Date: 2021-12-03
陕西中鸿瑞康健康管理有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the amount of triglycerides is not sensitive, and many patients only find out that the triglycerides exceed the standard after they have apparent symptoms, so they miss the best time for treatment and prevention

Method used

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  • Stem cell factor freeze-dried powder capable of reducing triglyceride as well as preparation method and application of stem cell factor freeze-dried powder
  • Stem cell factor freeze-dried powder capable of reducing triglyceride as well as preparation method and application of stem cell factor freeze-dried powder
  • Stem cell factor freeze-dried powder capable of reducing triglyceride as well as preparation method and application of stem cell factor freeze-dried powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A stem cell factor freeze-dried powder capable of reducing triglyceride is prepared according to the following steps:

[0036] The mesenchymal stem cells of the P0 generation were 1.0×10 5 / cm 2 Density seeding in cell culture flasks, adding mesenchymal stem cell medium for culture, when the density reaches 80%, the cell culture supernatant is collected under a sterile operating environment, the supernatant is centrifuged to remove cell debris, and then ultra- The concentrated solution was obtained by filtering the concentrated membrane bag and intercepting, and then freeze-dried at -40°C to obtain the dry cell factor freeze-dried powder, which was stored at room temperature. Each 1mL concentrated solution is stored in a vial, and then freeze-dried at -40°C and stored for later use. All the concentrated solutions are divided and freeze-dried.

[0037] The mesenchymal stem cell culture medium is α-MEM containing 10% fetal bovine serum by volume fraction;

[0038] Desc...

Embodiment 2

[0041] A stem cell factor freeze-dried powder capable of reducing triglyceride is prepared according to the following steps:

[0042] The mesenchymal stem cells of the P10 generation were 3.0×10 5 / cm 2 Density seeding in cell culture flasks, adding mesenchymal stem cell medium for culture, when the density reaches 95%, for example, collecting the cell culture supernatant in a sterile operating environment, the supernatant is centrifuged to remove cell debris, and then The ultrafiltration concentration membrane bag was cut off to obtain the concentrated solution, and then freeze-dried at -40°C to obtain the dry cell factor freeze-dried powder, which was stored at room temperature.

[0043] The mesenchymal stem cell culture medium is α-MEM containing 10% fetal bovine serum by volume fraction;

[0044] The centrifugation condition is 1500rpm, centrifugal 5min;

[0045] The cut-off molecular weight of the ultrafiltration concentration membrane bag is 5KD and 50KD. The supernatan...

Embodiment 3

[0047] A stem cell factor freeze-dried powder capable of reducing triglyceride is prepared according to the following steps:

[0048] The mesenchymal stem cells of the P3 generation were 1.2 × 10 5 / cm 2 Density seeding in cell culture flasks, adding mesenchymal stem cell culture medium for culture, when the density reaches 85%, collect the cell culture supernatant, such as in a sterile operating table environment, after the supernatant is centrifuged to remove cell debris, and then supernatant The concentrated solution was obtained by filtering the concentrated membrane bag and intercepting, and then freeze-dried at -40°C to obtain the dry cell factor freeze-dried powder, which was stored at room temperature.

[0049] The mesenchymal stem cell culture medium is α-MEM containing 10% fetal bovine serum by volume fraction;

[0050] The centrifugation condition is 1000rpm, centrifugal 8min;

[0051] The cut-off molecular weight of the ultrafiltration concentration membrane bag...

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Abstract

The invention belongs to the technical field of biological medicines, and particularly relates to stem cell factor freeze-dried powder capable of reducing triglyceride as well as a preparation method and application of the stem cell factor freeze-dried powder. The stem cell factor freeze-dried powder is obtained by concentrating and freeze-drying a supernatant of an umbilical cord mesenchymal stem cell culture solution, and has the effects of reducing triglyceride, glucose and I-type collagen and promoting liver cell proliferation.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a stem cell factor freeze-dried powder capable of reducing triglycerides, a preparation method and application thereof. Background technique [0002] Triglyceride (Triglyceride, TG), also known as triacylglycerol, is a fat molecule formed by long-chain fatty acids and glycerol. It is the most abundant lipid in the human body. Triglyceride can come from the decomposition of fat in food, and the liver can also Some sugars in the blood are converted into triglycerides, and most tissues can use the breakdown products of triglycerides to supply energy to the human body. Therefore, triglycerides are in a dynamic balance of synthesis and decomposition in the body. Once the balance is broken and there is excessive triglyceride in the body, hyperlipidemia will be formed, which will cause the disorder of the lipid metabolism system, further induce the disorder of liver meta...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61K9/19A61P3/06A61P3/10A61P1/16A61P5/50C12N5/0775A23L33/00
CPCA61K35/28A61K9/19A61K9/006A61P3/06A61P3/10A61P1/16A61P5/50C12N5/0662A23L33/00A23V2002/00A23V2200/3262A23V2200/328A23V2200/32
Inventor 董玲娟马嘉丽明磊国
Owner 陕西中鸿瑞康健康管理有限公司
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