Desulfurization and ammonia removal microbial deodorant and preparation method thereof

A technology for microorganisms and deodorants, applied in the field of microbial compositions, can solve the problems of unsatisfactory deodorization effect of deodorant microorganisms, easy mutation of deodorant microorganisms, easy interference by other miscellaneous bacteria, etc. High odor efficiency and the effect of inhibiting crop diseases and insect pests

Pending Publication Date: 2021-12-03
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] In addition, the traditional screening method is not very directional, and due to the limitations of the screening method and the specificity of the microorganisms, the deodorizing microorganisms are prone to mutation, poor environmental adaptability, and susceptible to interference from other bacteria. Unsatisfactory effect of deodorizing microorganisms

Method used

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  • Desulfurization and ammonia removal microbial deodorant and preparation method thereof
  • Desulfurization and ammonia removal microbial deodorant and preparation method thereof
  • Desulfurization and ammonia removal microbial deodorant and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment one: strain screening

[0023] In order to obtain efficient desulfurization and ammonia removal bacteria and avoid the interference of other miscellaneous bacteria, the present invention takes pig manure compost and pig manure and urine in different habitats as test materials, and conducts directional enrichment and domestication under the conditions of 28-35°C and 160-220r / min Desulfurization and ammonia removal microorganisms. First, put the collected samples in a certain concentration of ammonia selection liquid medium and basic inorganic salt medium containing sodium sulfide to "enrich and domesticate" desulfurization and ammonia removal microorganisms, subculture in turn to the 42nd generation, and dilute the enrichment solution Apply on Luria-Bertani medium (abbreviation: LB medium), pick a single colony, separate and purify by streaking on a plate, and finally combine each single strain and screen for desulfurization and ammonia removal to obtain an ef...

Embodiment 2

[0024] Embodiment two: Deodorization performance measurement of desulfurization and ammonia removal microbial deodorant

[0025] Bacillus altitudinis (GAU-00532) screened out in Example 1: Bacillus subtilis subsp.Stercoris (GAU-00533): Bacillus zhangzhouensis (GAU-00534) according to (0.8~1.2): (1.6~2.5): (1.0 ~1.8) mass ratio mixed.

[0026] The measurement of the composite strain system showed that there was no antagonistic reaction among the three strains, and the OD value was 0.764 after 12 hours of culture, which was 62%-108% higher than that of the three strains in the combination when they were cultured alone; the ammonia gas absorption rate was 79.8%, The hydrogen sulfide absorption rate is 60.5%, which is 2% to 41% higher than that of the three strains in the combination when the three strains are cultured separately (Table 1).

[0027] Table 1: Comparison of the main index content of the strain system after mixing with that of each strain before mixing

[0028] ...

Embodiment 3

[0030] Example 3: Preparation and application of microbial deodorant for desulfurization and ammonia removal

[0031] (1) Refrigerate strains in cryopreservation tubes → activate separately → primary expansion → secondary expansion → shaking table expansion → preparation → detection → liquid packaging → microbial deodorant; the specific preparation method is:

[0032]① Selection of microorganisms. The pig farm microbial deodorant has 3 strains of microorganisms, which belong to Acinetobacter pittii, Bacillus subtilis subsp. Stercoris, and Bacillus pumilus.

[0033] ②Activation, take the above-mentioned 3 strains of bacterial cryopreservation tubes out of the -80°C refrigerator and immediately place them in a 38°C-40°C water bath for rapid recovery and shake them properly until the internal ice is completely dissolved. Generally, it takes about 50 seconds to 100 seconds. second. Under sterile conditions, clean the cryopreservation tube with absorbent cotton soaked in 75% alcoh...

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Abstract

The invention relates to a microbial composition, in particular to a microbial composition with a deodorization effect. The microbial composition consists of Bacillus altitudinis (GAU-00532), Bacillus subtilis subsp. Stercoris (GAU-00533) and Bacilluszhangzhouensis (GAU-00534), the Bacillus altitudinis (GAU-00532), the Bacillus subtilis subsp. Stercoris (GAU-00533) and the Bacilluszhangzhouensis (GAU-00534) are all collected in the China Center for Type Culture Collection, the collection address is Wuhan University, Wuhan, and the collection numbers are respectively CCTCC NO: M 2021352, CCTCC NO: M 2021350 and CCTCC NO: M2021351. The desulfurization and ammonia removal microbial deodorant is low in cost, small in dosage and high in deodorization efficiency, and is free of chemical additives and complex control systems, and deodorization products can be directly utilized and do not need to be treated again.

Description

technical field [0001] The invention relates to a microbial composition, in particular to a microbial composition with deodorizing effect. Background technique [0002] With the improvement of people's living standards and the continuous expansion of the demand for meat, the rapid development of the livestock and poultry industry has been promoted, but most of the farms do not have perfect excrement treatment facilities, which not only causes the surrounding environment to smell bad , sewage flow and the breeding of mosquitoes and flies, and the pollution of organic matter and pathogenic bacteria in soil and water is becoming more and more serious. The odor of pig farms mainly comes from the smell of pigs, the gas discharged from the respiratory tract and the excrement of the body, and the excrement of the body is the main source of the odorous gas. The excrement of livestock and poultry contains undigested organic matter. Substances, such as starch, fat, protein, non-starc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F11/02C05F17/80C05F3/00C05G3/80C12R1/07C12R1/125C02F101/10C02F101/16
CPCC12N1/20C02F11/02C05F17/80C05F3/00C05G3/80C02F2101/101C02F2101/16Y02A40/20Y02W30/40
Inventor 姚拓李昌宁
Owner GANSU AGRI UNIV
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