Hybridoma cell strain capable of secreting bisamides resistant monoclonal antibody and application thereof
A hybridoma cell line, monoclonal antibody technology, applied in the field of immunochemistry, can solve the problems of expensive equipment, high solvent, consumption and so on
Active Publication Date: 2021-12-03
JIANGNAN UNIV
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Abstract
The invention provides a hybridoma cell strain capable of secreting a bisamides resistant monoclonal antibody and an application thereof, and belongs to the technical field of immunochemistry. The preservation number of the hybridoma cell strain is CGMCC No.22322. The preparation method comprises the following steps: mixing and emulsifying a complete antigen and a Freund's adjuvant, carrying out subcutaneous immunization on a mouse, fusing splenocytes from the high-titer and low-IC50 mouse with myeloma cells of the mouse through a PEG method, and screening out hybrid cells after fusing the two cells by adopting a selective culture medium; and screening cells by an indirect competitive enzyme-linked immunosorbent assay and subcloning for three times to finally obtain the hybridoma cell strain secreting the monoclonal antibody The monoclonal antibody secreted by the cell strain has good detection sensitivity to cyclaniliprole, cyantraniliprole, chlorantraniliprole and tetrachlorantraniliprole, and can be used for detecting residues of the four pesticides in foods.
Application Domain
Biological material analysisFused cells +5
Technology Topic
Molecular biologySplenocyte +14
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