The invention discloses a
hybridoma cell strain JS secreting an anti-
phenolphthalein monoclonal antibody and application of the
hybridoma cell strain JS, and belongs to the field of
food safety immunodetection. The
hybridoma cell strain JS secreting the anti-
phenolphthalein monoclonal antibody is preserved in the China General
Microbiological Culture Collection Center (CGMCC), the preservation date is March 7, 2019, and the preservation number is CGMCC No.17394. According to the hybridoma
cell strain JS secreting the anti-
phenolphthalein monoclonal antibody and the application of the hybridoma
cell strain JS, a complete
antigen of phenolphthalein and a Freund's
adjuvant with the amount the same as that of the complete
antigen are subjected to mixed emulsification, and a BALB / c mouse issubjected to subcutaneous multi-point injection at the nape part for being immunized. An indirect competitive euzymelinked immunosorbent
assay is adopted for screening cells,
subcloning is conductedthree times, and finally, the
monoclonal antibody hybridoma
cell strain is obtained. The
monoclonal antibody secreted by the
cell strain has good specificity and detection sensitivity (the IC50 valueis 15.4 ng / mL) for the phenolphthalein, the amount of phenolphthalein residuals in healthcare food can be detected, the
raw material is provided for immunodetection for the phenolphthalein residuals in food, and the hybridoma
cell strain JS secreting the anti-phenolphthalein
monoclonal antibody has practical application value.