Hybridoma cell strain CBC secreting monoclonal antibody of dicofol and application of hybridoma cell strain CBC

A hybridoma cell line, dicofol technology, applied in analytical materials, biochemical equipment and methods, instruments and other directions, can solve problems such as excessive residues, high solvent consumption, aquatic toxicity, etc., and achieve the effect of good detection sensitivity
CN110607283AInactive Publication Date: 2019-12-24江苏权正检验检测有限公司 +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
江苏权正检验检测有限公司
Publication Date
2019-12-24
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a hybridoma cell strain CBC secreting a monoclonal antibody of dicofol and application of the hybridoma cell strain CBC, and belongs to the field of food safety immunoassay. The cell strain CBC is preserved in the China General Microbiological Culture Collection Center, the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Beichen West Road, ChaoyangDistrict, Beijing, the preservation date is March 7, 2019, and the preservation number is CGMCC No.17398. Complete antigens of the dicofol are mixed and emulsified with equivalent freund adjuvants, and BALB / c mice are immunized by subcutaneous multipoint injection of neck and back through first immunization, multiple strengthening immunization and sprint immunization. Spleen cells of the mice withhigh titer and low IC50 are fused with mouse myeloma cells by a PEC method, selective culture is conducted, then cells are screened and sub-cloned for three times through indirect competitive enzyme-linked immunosorbent assay, finally the cell strain CBC is obtained, the monoclonal antibody secreted by the cell strain CBC has good detection sensitivity to the dicofol (value of the IC50 is 19.09 ng / mL), and can be used for the detection of dicofol residues in food.
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Description

technical field

[0001] The invention relates to a hybridoma cell line CBC secreting dicofol monoclonal antibody and application thereof, which belongs to the field of food safety immunological detection. Background technique

[0002] Dicofol [2,2,2-trichloro-1,1-bis(4-chlorophenyl)ethanol], the English name dicofol (hereinafter abbreviated as DCF), is a broad-spectrum, low- Toxic organochlorine acaricide, which has contact and stomach poisoning effects on mites, and is effective on adults, larvae and eggs. The production and synthesis of dicofol uses DDT as the raw material. During the production process, the conversion rate of the chlorination section is generally about 70%. If the subsequent purification is not carried out or the purification is not complete, the finished product will contain DDT to varying degrees.

[0003] Extensive use of dicofol will lead to excessive residues. In recent years, more and more evidences have shown that its exposure in the environment ha...

Claims

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