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Sargassum pallidum fucosan as well as preparation method and application thereof

A technology of fucoidan and artemisia fucus, which is applied in the field of natural polymers, can solve the problems of extraction and purification and anti-H1N1 influenza virus research that have not been reported, and achieve the effect of increased anti-H1N1 influenza virus activity and high purity

Active Publication Date: 2021-12-07
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the extraction and purification of fucoidan and its degraded polysaccharides from Artemisia chinensis and the research on anti-H1N1 influenza virus

Method used

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  • Sargassum pallidum fucosan as well as preparation method and application thereof
  • Sargassum pallidum fucosan as well as preparation method and application thereof
  • Sargassum pallidum fucosan as well as preparation method and application thereof

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preparation example Construction

[0034] The present invention provides the preparation method of above-mentioned sea wormwood fucoidan, comprises the following steps:

[0035] (1) Degreasing: reflux the sea wormwood powder with 95% ethanol at 75-90°C for 3-5h, repeat 2-3 times, remove lipid and dry the residue;

[0036] (2) Hot water extraction: The degreased dry powder is extracted with hot water at 70-90°C for 2-3 hours according to the mass-volume ratio of material to liquid at 1:10-50, repeated 2-3 times, centrifuged, and all supernatants are collected. concentrate;

[0037] (3) CaCl 2 Alginate removal: 3mol / L CaCl 2 Settle at 4°C for 8-12 hours, repeat 1-2 times, centrifuge, dialyze, concentrate and freeze-dry the supernatant to obtain crude sea sage fucoidan;

[0038] (4) Purification: The crude sea wormwood fucoidan was chromatographed on the Q-Sepharose Fast Flow column, and deionized water and NaCl solution (0.5, 1, 1.5, 2mol / L) were eluted with a gradient of 3 mL / min, and each Connect 6mL tubes...

Embodiment 1

[0046] Distill the sea wormwood powder with 95% ethanol at 85°C for 4h (3 times) to remove the lipid and dry the residue, pulverize; the obtained powder was extracted with 80°C hot water at a solid-liquid ratio of 1:30 for 3h (repeat 3 times), centrifuge, collect all supernatant, concentrate; add CaCl 2 to 3 mol / L, the sedimentation temperature was 4°C, and stood for 10 hours, then centrifuged, dialyzed, concentrated and freeze-dried the supernatant to obtain crude sea wormwood fucoidan.

[0047] Crude fucoidan was chromatographed on Q-Sepharose Fast Flow column, eluted with deionized water and NaCl solution (0.5, 1, 1.5, 2mol / L) at a gradient of 3mL / min, each tube was connected with 6mL, each flow Connect 60 tubes, collect the main polysaccharide eluted with 1.5mol / L NaCl, and purify it with Sepharose 4B Fast Flow column to obtain Artemisia fucoidan, which is named SF0.

[0048] SF0 was treated with 0.1mol / LTFA at 60°C for 3h. Low-molecular-weight oligosaccharides were remo...

Embodiment 2

[0050] Distill the sea wormwood powder with 95% ethanol at 90°C for 5h (twice) to remove the lipid and dry the residue, pulverize; the obtained powder was extracted with 75°C hot water at a solid-liquid ratio of 1:40 for 2h (repeat 3 times), centrifuge, collect all supernatant, concentrate; add CaCl 2 to 3 mol / L, the sedimentation temperature was 4°C, stood for 12 hours, then centrifuged, dialyzed, concentrated and freeze-dried the supernatant to obtain crude sea wormwood fucoidan.

[0051] Crude fucoidan was chromatographed on Q-Sepharose Fast Flow column, eluted with deionized water and NaCl solution (0.5, 1, 1.5, 2mol / L) at a gradient of 3mL / min, each tube was connected with 6mL, each flow Connect 60 tubes. The main polysaccharide eluted with 1.5 mol / L NaCl was collected and purified with a Sepharose 4B Fast Flow column to obtain sea wormwood fucoidan, which was named SF0.

[0052] SF0 was treated with 0.15mol / LTFA at 55°C for 2h. Low-molecular-weight oligosaccharides we...

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Abstract

The invention discloses sargassum pallidum fucosan as well as a preparation method and application thereof. The sulfate radical content of the fucosan is 9-13%, the relative molecular mass is 9-15kDa, the fucosan comprises 45-55% by mole of mannose, 40-50% by mole of glucuronic acid and 0.1-5% by mole of galactose, and the fucosan has H1N1 influenza virus resisting activity. The preparation method of the sargassum pallidum fucosan comprises the steps of degreasing, hot water extraction, alginic acid removal by CaCl2, purification, and gradual partial acid hydrolysis. The sargassum pallidum fucosan prepared through the purification and the gradual partial acid hydrolysis is high in purity, does not contain impurities such as protein and nucleic acid, has high H1N1 influenza virus resisting activity, and can be used for preparing anti-H1N1 influenza virus drugs.

Description

technical field [0001] The invention relates to the technical field of natural polymers, in particular to a sea wormwood fucoidan with anti-H1N1 influenza virus activity and its preparation method and application. Background technique [0002] Sargassum pallidum, a brown alga belonging to the genus Sargassum, is widely distributed in the Yellow Sea and East China Sea of ​​China, and has been used as a Chinese herbal medicine. Its biological activity and degree of sulfation, molecular weight, monosaccharide composition, and main polymer chain have been reported structure are closely related. "Dictionary of Traditional Chinese Medicine" records that Artemisia annua is bitter, salty and cold in nature, enters the lung, spleen and kidney meridian, has the effects of softening firmness, eliminating phlegm, diuresis and purging heat, and can be used to treat scrofula, gall tumors, accumulation, edema, Symptoms such as athlete's foot and testicular swelling and pain. Fucoidan is ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61K36/03A61P31/16
CPCC08B37/0003C08B37/006A61K31/715A61K36/03A61P31/16Y02A50/30
Inventor 王培培薛卫李玲翠吴文惠包斌
Owner SHANGHAI OCEAN UNIV
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