Method for collecting living glandular hairs of plant leaves

A plant leaf and plant technology, applied in the agricultural field, can solve the problems of poor precision and low efficiency, and achieve the effect of retaining integrity and specificity

Pending Publication Date: 2021-12-07
SHANGHAI JIAO TONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The invention provides a method for collecting living glandular hairs of plant leaves, which is used to overcome the problems of poor precision and low efficiency in the prior art in the process of collecting glandular hairs

Method used

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  • Method for collecting living glandular hairs of plant leaves
  • Method for collecting living glandular hairs of plant leaves
  • Method for collecting living glandular hairs of plant leaves

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Embodiment 1

[0036] This embodiment provides a method for collecting living glandular hairs of Artemisia annua. Artemisia annua L. is an annual herb of the genus Artemisia in the Asteraceae family. The plant has a strong volatile odor. Artemisia annua contains a variety of secondary metabolites, such as: artemisinin, β-caryophyllene, camphor, β bamboo farnesene and so on. Artemisinin (Artemisinin) is a sesquiterpene lactone compound containing a peroxo bridge structure isolated from its leaves. It is currently recognized as the most effective drug for treating malaria in the world, especially for cerebral malaria and anticancer drugs. Chloroquine is more effective for malaria. In recent years, artemisinin and its derivatives have also been reported to have the effects of lupus erythematosus, tuberculosis, diabetes, anti-tumor, lowering blood fat, and treating Alzheimer's disease. Therefore, the separation of other secondary metabolites such as artemisinin and its derivatives in Artemisia...

Embodiment 2

[0043] The present embodiment provides a kind of collection method of tomato live glandular hair, specifically comprises the following steps:

[0044] 1. Select 6-8 weeks of healthy mature tomato leaves. Because the water content of the tomato is relatively high, the leaves are relatively fragile. After scratching the epidermis of the mature tomato plant leaves with a scalpel, stick the adhesive tape on the epidermis and tear it to obtain Single layer epidermis, tomato single layer epidermis such as image 3 shown;

[0045] 2. Place the tomato single-layer epidermal tissue on a glass slide covered with a PEN film, extend and add distilled water to the tomato plant single-layer epidermal tissue;

[0046] 3. Place the glass slide carrying the single-layer epidermal tissue of the tomato plant under the laser microdissection system, and cut after finding the living glandular hairs. The single-layer epidermal tissue after cutting is as follows: Figure 4 As shown, the collected l...

Embodiment 3

[0048] This embodiment provides a method for collecting glandular hairs of Nicotiana benthamiana. Nicotiana tabacum L. is a plant of the genus Nicotiana in the family Solanaceae. It is widely used in plant basic scientific research and industrial production. The whole plant can be used as a pesticide and insecticide, and can also be used medicinally for anesthesia, sweating, sedation and emetic. agent. Ben's tobacco leaves contain nicotine (nicotine). After harvesting, they are modulated, graded and processed to make tobacco products, etc. It is a hobby industrial raw material crop cultivated worldwide. Nicotiana benthamiana has two types of multicellular glandular hairs, the so-called long hairs and short hairs. Long glandular trichomes are known to secrete various alkaloids, including nicotine, while short glandular trichomes can secrete a variety of terpenoids. The two types of glandular trichomes of Nicotiana benthamiana co-produce a variety of compounds, including terpe...

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Abstract

The invention provides a method for collecting living glandular hairs of plant leaves. The method comprises the following steps: selecting mature plant leaves to obtain plant monolayer epidermal tissues, cutting the living glandular hairs in the plant monolayer epidermal tissues under a laser microdissection system, and collecting and obtaining the living glandular hairs. The invention provides a method for collecting living glandular hairs of plant leaves, which comprises the following steps: separating and cutting living glandular hair structures on mature plant leaves, and completely retaining and collecting the living glandular hair of the plant leaves under the conditions of not damaging cell structures and not adding exogenous chemical reagents. Completeness and specificity of living glandular hair are reserved to a great extent; meanwhile, the method provided by the invention is suitable for various plants, and provides experimental conditions for researches such as exploration of secondary metabolites in plant glandular hairs and related synthesis paths thereof.

Description

technical field [0001] The invention relates to the technical field of agriculture, in particular to a method for collecting living glandular hairs of plant leaves. Background technique [0002] Plant glandular trichomes are special plant structures that are specialized from the epidermal cells of the terrestrial parts of plants. They are generally composed of basal cells, stalk cells, and secretory cells. They are widely found in medicinal plants of Labiatae, Solanaceae, and Asteraceae. middle. Plant glandular hairs play an important role in plant secondary metabolism and plant stress resistance. For example, abundant secondary metabolites are accumulated in living plant glandular hairs, including terpenes, phenylpropanes, flavonoids, methyl ketones, Compounds such as acyl sugars, and these compounds are widely used in various fields such as pharmaceutical production, food, biological pesticides, flavoring agents, spices, etc., and have great application value. It is part...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/04
CPCG01N1/04
Inventor 唐克轩秦维李勇鹏彭柏文孙光新
Owner SHANGHAI JIAO TONG UNIV
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