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Leukocyte binding C reactive protein rapid detection card and preparation method thereof

A technology of reactive protein and detection card, which is applied in the field of protein immunological detection, can solve the problems of elevated white blood cell count and inability to completely rule out infection, etc., and achieve the effect of simple detection operation, fast and accurate quantitative detection results, and improved accuracy

Inactive Publication Date: 2021-12-07
北京倍肯恒业科技发展股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In other words, just based on the increase or decrease of the white blood cell count, it cannot be sure that an infection has occurred, and the normal white blood cell count cannot completely rule out the occurrence of infection

Method used

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  • Leukocyte binding C reactive protein rapid detection card and preparation method thereof
  • Leukocyte binding C reactive protein rapid detection card and preparation method thereof
  • Leukocyte binding C reactive protein rapid detection card and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] In the present embodiment, the preparation solution involved includes:

[0067] Reagent card hydrophilic treatment solution: 10mM PBS buffer + 1% TW20;

[0068] Gold-labeled antibody complex solution: 10mM borate buffer + 6% sucrose + 2% BSA;

[0069] Glass fiber membrane pretreatment solution: 50Mm borate buffer + 0.3% TW20 + 2% BSA;

[0070] Antibody coating solution: 10mM PBS buffer + 3% methanol;

[0071] pH regulator: 1% potassium carbonate solution;

[0072] Blocking solution: 10% BSA aqueous solution.

[0073] The leukocyte-binding C-reactive protein rapid detection component of the present invention utilizes blood cell staining and counting and C-reactive protein immunochromatographic reaction principles to simultaneously realize rapid quantitative detection of leukocytes and C-reactive protein in whole blood samples. The leukocyte-binding C-reactive protein rapid detection component includes a detection card 1 and a sample diluent, the sample diluent is con...

Embodiment 2

[0086] Embodiment 2 performance detection test

[0087] In this example, the detection limit, analytical specificity, accuracy, hook effect, precision, and linear range of the leukocyte-binding C-reactive protein rapid test card were respectively tested to evaluate whether they meet the requirements of the experimental design. .

[0088]In this embodiment, the standard products used for performance evaluation are human hypersensitive C-reactive protein No. 8C72 of Millipore Company and CELL-DYN 29Plus Control of Abbott Company for blood cell analysis. All experiments require the experimenters to be familiar with the detection method and instrument operation; use appropriate standards; the reagents used in the experiment should be within the validity period.

[0089] 1. Detection limit (this performance evaluation is for the C-reactive protein detection reagent in the kit)

[0090] The sample treatment solution in the kit in Example 1 was used as the blank solution, and the b...

Embodiment 3

[0160] Embodiment 3 Clinical Serum Sample Detection

[0161] Experimenters should be familiar with the detection method and instrument operation; the reagents used in the experiment should be of the same batch number and within the validity period. The whole blood used in the experiment is freshly collected, and has been clinically tested to determine the assigned value.

[0162] Randomly select 0.5mg / L9 / L9 / L whole blood samples of 30 cases, 12mg / L>C-reactive protein content>3mg / L, 18×10 9 / L>Total leukocytes>10mg / ml×10 9 70 cases of / L whole blood samples were added dropwise to the test card in Example 1 for blind test and the statistical results are shown in Table 16-17 below.

[0163] Table 16 C-reactive protein test results

[0164]

[0165]

[0166]

[0167] Table 17 Total white blood cell test results

[0168]

[0169]

[0170] It can be seen that the clinical accuracy of the detection card of the present invention is CV<15%, which meets the needs of...

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Abstract

The invention belongs to the technical field of protein immunodetection, particularly relates to a leukocyte-binding C-reactive protein rapid detection card, and further discloses a preparation method and application of the leukocyte-binding C-reactive protein rapid detection card. According to the leukocyte and C-reactive protein combined rapid detection assembly, the leukocyte bin and the C-reactive protein test paper card are respectively arranged, and the leukocyte and C-reactive protein in a whole blood sample can be rapidly and quantitatively detected at the same time according to the blood cell staining counting and C-reactive protein immunochromatography reaction principle; the accuracy of diagnosing infection is greatly improved, and the method has important significance for identifying bacterial and virus infection.

Description

technical field [0001] The invention belongs to the technical field of protein immunoassay, in particular to a leukocyte-binding C-reactive protein rapid detection card, and further discloses its preparation method and application. Background technique [0002] C-reactive protein (CRP) is a typical acute phase protein. Under normal circumstances, the content of CRP is very small, but its blood concentration will increase sharply during acute trauma and infection. Usually, in healthy people, the CRP in the serum of newborns is less than 2mg / L, and the CRP in the serum of children and adults is less than or equal to 10mg / L. However, within 4-8 hours when the human body develops inflammatory diseases or tissue ulceration or necrosis However, the CRP value can rise sharply to about 20-500mg / L, and with the recovery of tissue structure and function, the CRP content value will also return to normal levels. Therefore, based on the level of CRP in the blood, it can be used to evalu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/569G01N33/558G01N33/58G01N33/543
CPCG01N33/6893G01N33/577G01N33/56911G01N33/56983G01N33/558G01N33/587G01N33/54346G01N2333/4737G01N2800/32G01N2800/2871
Inventor 关苗丁绍伟赵洋张学治李兆青
Owner 北京倍肯恒业科技发展股份有限公司
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