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Preparation method of microbial fermentation bacteria

A technology of microbial fermentation bacteria and Lactobacillus sporogenes, applied in the field of microorganisms, can solve the problems of low fermentation efficiency, consumption of nutrients, insufficient fermentation of feces, etc., and achieve the effect of eliminating odor and increasing the contact area

Active Publication Date: 2021-12-14
烟台上水医药科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in the existing technology, there are various problems such as insufficient feces fermentation, excessive fermentation consumes nutrients, very smelly feces affects the environment, and low fermentation efficiency, which restricts the large-scale application of manure fermentation to prepare organic fertilizers.

Method used

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  • Preparation method of microbial fermentation bacteria
  • Preparation method of microbial fermentation bacteria

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Embodiment 1

[0021] A preparation method for microbial fermentation bacteria, characterized in that it comprises the steps of:

[0022] According to the mass parts, mix 12 parts of Lactobacillus subtilis, 10 parts of Bacillus coagulans, 13 parts of Eubacterium elongatus, 18 parts of Acetobacter xylinum, and 15 parts of Trichoderma reesei powder, and respectively activate and cultivate them in the culture medium , and then inoculated into a fermenter for expanded cultivation, mixed the obtained strains into 500 parts of surface-modified porous activated carbon, and mixed evenly to obtain microbial fermentation bacteria.

[0023] The preparation method of described modified porous activated carbon is:

[0024] 1000 parts of activated carbon were pulverized, sieved to obtain 150-mesh powder particles, washed with ultrasonic waves for 10 minutes, washed repeatedly 3 times, and then placed in an oven at 100°C for 4 hours to fully remove water to obtain pretreated activated carbon;

[0025] Add...

Embodiment 2

[0027] A preparation method for microbial fermentation bacteria, characterized in that it comprises the steps of:

[0028] According to the number of parts by mass, mix 15 parts of Lactobacillus subtilis, 12 parts of Bacillus coagulans, 16 parts of Eubacterium elongatus, 20 parts of Acetobacter xylinum, and 18 parts of Trichoderma reesei powder, and respectively activate and cultivate them in the culture medium , and then inoculated into a fermenter for expanded cultivation, mixed the obtained strains into 520 parts of surface-modified porous activated carbon, and mixed evenly to obtain microbial fermentation bacteria.

[0029] The preparation method of described modified porous activated carbon is:

[0030] 1,000 parts of activated carbon were pulverized, sieved to obtain 160-mesh powder particles, washed with ultrasonic waves for 15 minutes, washed repeatedly 5 times, and then placed in an oven at 120°C for 5 hours to fully remove water to obtain pretreated activated carbon;...

Embodiment 3

[0033] A preparation method for microbial fermentation bacteria, characterized in that it comprises the steps of:

[0034] According to the number of parts by mass, mix 19 parts of Lactobacillus subtilis, 18 parts of Bacillus coagulans, 22 parts of Eubacterium elongatus, 20 parts of Acetobacter xylinum, and 19 parts of Trichoderma reesei powder, and respectively activate and cultivate them in the culture medium , and then inoculated into a fermenter for expanded cultivation, mixed the obtained strains into 580 parts of surface-modified porous activated carbon, and mixed evenly to obtain microbial fermentation bacteria.

[0035] The preparation method of described modified porous activated carbon is:

[0036] 1000 parts of activated carbon were pulverized, sieved to obtain 200-mesh powder particles, washed with ultrasonic waves for 40 minutes, washed repeatedly for 3-8 times, and then placed in an oven at 130°C for 6 hours to fully remove water to obtain pretreated activated ca...

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Abstract

The invention discloses a preparation method of microbial fermentation bacteria, and relates to the field of microorganisms. The preparation method comprises the following steps of: uniformly mixing 12-28 parts by mass of bacillus subtilis, 10-30 parts by mass of bacillus coagulans, 13-27 parts by mass of eubacterium elongatum, 18-24 parts by mass of acetobacter xylinum and 15-25 parts by mass of trichoderma reesei powder, respectively performing activation culture in a culture medium, then inoculating the mixture into a fermentation tank for amplification culture, mixing the obtained strains, adding the mixture into 500-600 parts by mass of surface-modified porous activated carbon, and performing uniform mixing to obtain the microbial fermentation bacteria. According to the preparation method disclosed by the invention, the pretreated activated carbon is added into the system, so that the microbial fermentation bacteria are uniformly loaded into pores of the activated carbon, and the contact area between the microbial fermentation bacteria and odor molecules, especially volatile gas, is increased.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a method for preparing microbial fermentation bacteria. Background technique [0002] Animal manure is rich in organic matter and nutrients needed for crop growth, including cellulose, hemicellulose, lignin, protein and its decomposition components, fat, organic acids, enzymes and various inorganic salts, which are beneficial to increasing crop yield and improving Soil fertility has a good effect. If animal manure is directly used for fertilization, many internal nutrients cannot be directly absorbed by crops. The macromolecular substances in manure need to be fermented and degraded before they can be slowly absorbed by plant roots. The process is very slow, and manure also contains many pathogenic bacteria, such as Escherichia coli, nematodes, etc., direct use will harm plant growth, feces will be fermented in the soil, and fermentation will generate a lot of heat, during which ha...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C12N11/14C01B32/354C05F17/20C05F3/00C12R1/07C12R1/01C12R1/885
CPCC12N1/20C12N1/14C12N11/14C01B32/354C05F17/20C05F3/00Y02A40/20Y02W30/40
Inventor 崔明
Owner 烟台上水医药科技有限公司