Method for measuring activity of formate dehydrogenase in plant leaves and evaluating activity of formate dehydrogenase

A formate dehydrogenase and plant leaf technology, applied in biochemical equipment and methods, chemical method analysis, microbial measurement/inspection, etc., can solve problems such as complex operation, long duration, slow formaldehyde release process, etc., and achieve a solution Complicated operation, solve the effect of complicated operation steps

Pending Publication Date: 2021-12-21
XINJIANG UNIVERSITY
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

The commonly used methods to remove indoor formaldehyde can be divided into fiber filtration, membrane separation, electrostatic supplementation, activated carbon adsorption and ozone sterilization, etc. Due to the characteristics of slow formaldehyde release process and long duration, some commercialized technologies have complicated operation and high cost. High, there may also be a risk of secondary pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Based on the evaluation of the activity of formaldehyde dehydrogenase in the leaves of Chlorophytum peony and Chlorophytum

[0026] (1) Prepare to pipette 20.00 mL of fresh or inactivated Peony Chlorophytum leaf extract into four 100 mL centrifuge tubes, then add 8.00 mL of water and formaldehyde solution concentration (350 mg·L -1 , 500 mg·L -1 , 750 mg·L -1 ), put in a magnet to fully stir the mixture on a magnetic stirrer for 1 min, and record the pH value of the initial solution. Then use the accurately calibrated 0.0250 mol·L -1 Sodium hydroxide solution, while titrating, use an acidity meter to test and record the pH value of the mixed solution after the reaction is complete. When the pH value of the mixed solution is close to about 11, stop the titration; all experiments are set in three parallels;

[0027] (2) Pipette 0.50 mL of fresh or inactivated Peony Chlorophytum leaf extract into 12 stoppered colorimetric tubes, add 0 mg·L -1 , 350 mg·L -1 ,...

Embodiment 2

[0029] Example 2 Based on the evaluation of formaldehyde dehydrogenase activity in green radish leaves

[0030] Similar to Example 1, the difference is that the plants in steps (1) and (2) are replaced with pothos of the same size and growth, and the fresh extract of the leaves of the pothos and the inactivated extract are respectively measured to add formaldehyde purification amount and acidity change value . The results show that when the ratio of the mass of green radish leaves to the extract is 1:4, the concentration of added formaldehyde is 350-750 mg.L -1 When the formate dehydrogenase activity is 1248~127 μg.g -1 FW; when the ratio of the mass of green radish leaves to the extract is 1:10, the concentration of added formaldehyde is 350~750 mg.L-1 When the formate dehydrogenase activity is 335~0 μg.g -1 FW; when the ratio of the mass of green radish leaves to the extract is 1:20, the concentration of added formaldehyde is 350~750 mg.L -1 When the formate dehydrogena...

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PUM

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Abstract

The invention discloses a method for measuring the activity of formate dehydrogenase in plant leaves and evaluating the activity of formate dehydrogenase. The activity of formate dehydrogenase in the leaf extracting solution is eliminated in a high-temperature treatment manner, and according to the chemical property of formic acid, a potentiometric titration method is adopted to quantitatively evaluate the activity of formate dehydrogenase in plant leaves through measuring the reduction value of the formaldehyde content in the plant fresh extracting solution and the inactivated extracting solution and the acidity change difference in the plant fresh extracting solution and the inactivated extracting solution before and after the formaldehyde is added. Experimental results show that when the ratio of the plant mass to the extracting solution is 1: 4 and the formaldehyde concentration is 350 mg.L <-1>, the activity of the formate dehydrogenase is sequentially as follows: the activity of the scindapsus aureus is greater than that of peony chlorophytum comosum, and the activity of the peony chlorophytum comosum is greater than that of aloe; when the formaldehyde concentration is 500-750 mg.L <-1>, the activity of the formate dehydrogenase is as follows: the activity of the peony chlorophytum comosum is greater than that of the scindapsus aureus, and the activity of the formate dehydrogenase is not detected in the aloe leaf extracting solution.

Description

technical field [0001] The invention relates to a method capable of measuring the activity of formaldehyde dehydrogenase and its evaluation, in particular to a potentiometric titration method for measuring the activity of formate dehydrogenase in plant leaves. Background technique [0002] Formaldehyde is one of the most important pollutants in indoor air, which seriously endangers human health. The commonly used methods to remove indoor formaldehyde can be divided into fiber filtration, membrane separation, electrostatic supplementation, activated carbon adsorption and ozone sterilization, etc. Due to the characteristics of slow formaldehyde release process and long duration, some commercialized technologies have complex operations and cost High, there may also be a risk of secondary pollution. Compared with other purification technologies, plant purification technology has the advantages of simple operation, no pollution, low cost, low energy consumption, and can also pla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/32G01N31/16
CPCC12Q1/32G01N31/164G01N2333/904
Inventor 苏玉红何晓红杨玉霞李晓娟
Owner XINJIANG UNIVERSITY
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